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Addgene

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We narrowed to 151 results for: 199

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  4. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...enzyme complex. . Schwarz DS et. al. Cell . 2003. 115:199-208. PubMed . Lentivirus-delivered stable gene silencing...Dulbecco’s Modified Eagle Medium (DMEM) Invitrogen: #11995 Fetal Bovine Serum (FBS) Invitrogen: #16000 Penicillin... lentiviral vector . Naldini L et. al. Science . 1996 272:263-267. PubMed . Asymmetry in the assembly ...delivery in vivo. Zufferey R et. al. Nat Biotechnol . 1997. 15(9):871-5. PubMed . Self-inactivating lentivirus...vivo gene delivery. . Zufferey R et. al. J Virol . 1998. 72(12):9873-80. PubMed . H.2. Web resources Addgene...

  5. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...improves infectious titer and yield." Gene therapy 6.6 (1999): 973-985 (Link opens in a new window) . Figure ...improves infectious titer and yield." Gene therapy 6.6 (1999): 973-985 (Link opens in a new window) . Right panel...

  6. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...nonmuscle myosin II (Karess et al., 1991, Jordan and Karess, 1997), which contains the regulatory regions... gene was aptly named groucho (Fisher and Caudy, 1998). Interested in plasmids that contain the groucho...it was aptly named Sleeping Beauty (Ivics et al., 1997). We have a lot of plasmids that contain components...those from the original publication, Ivics et al 1997. Spaghetti Squash, a regulator of cellular contraction...developing Drosophila embryos (Azplazu and Frasch, 1993).  Tinman, which is spatially restricted to the ...types in the developing embryo (Azplazu and Frasch, 1993). Tinman is specifically involved in the formation...defective tinman gene fail to develop a heart (Bodmer, 1993). Bodmer named the gene after the Wizard of Oz character...

  7. An Introduction to Adenovirus

    Type
    Blog Post
    ...the virus from the cell population (Kafri et al., 1998). In fact, adenovirus gets its name because it was...apparently contribute to virulence (Tollefson et al., 1996) – cell death has been reported when using vectors...existing for that adenoviral vector (Hofmann et al., 1999).  The ability of the adenovirus particle to activate...Cichon, G., Arnold, W., Both, G. W., & Strauss, M. (1999). Ovine Adenovirus Vectors Overcome Preexisting ...6930–6936. https://doi.org/10.1128/JVI.73.8.6930-6936.1999. PMID: 10400791. Kafri, T., Morgan, D., Krahl,...Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response to adenoviral vector .... W., Ryerse, J. S., Wold, L. J., & Wold, W. S. (1996). The adenovirus death protein (E3-11.6K) is required...

  8. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...Summers & Sherratt, 1984; Summers et al., 1993; Summers, 1998). Multimeric plasmids are also considered...stability. Mol Microbiol. 1998 Sep;29(5):1137-45. doi: 10.1046/j.1365-2958.1998.01012.x. PMID: 9767582. Summers...hypothesis. Mol Microbiol. 1993 Jun;8(6):1031-8. doi: 10.1111/j.1365-2958.1993.tb01648.x. PMID: 8361350....frequency (Summers & Sherratt, 1984; Summers et al., 1993). These larger plasmids are also maintained at lower...multimers (Summers & Sharratt, 1984; Summers et al., 1993). In these cases, screening multiple bacterial colonies...

  9. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...Proceedings of the National Academy of Sciences 88.1 (1991): 189-193. PubMed PMID: 1986365. PubMed Central ...thermostable DNA ligase-encoding gene." Gene 109.1 (1991): 1-11. PubMed PMID: 1756968. 7. Lauer, G., et al...coli DNA ligase." Journal of bacteriology 173.16 (1991): 5047-5053. PubMed PMID: 1840584. PubMed Central...thermophilus DNA ligase." Nucleic acids research 24.15 (1996): 3079-3085. PubMed PMID: 8760897. PubMed Central... reaction in a PCR world." PCR Methods Appl 1.1 (1991): 5-16. PubMed PMID: 1842922. 13. Wiedmann, M., ...overview and applications." PCR Methods Appl 3.4 (1994): S51-64. PubMed PMID: 8173509. 14. Zirvi, Monib...repeat sequences." Nucleic acids research 27.24 (1999): e40-e47. PubMed PMID: 10572192. PubMed Central...

  10. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ... referred to as “complex retroviruses ” (Cullen, 1992) because of their more complicated genomes. Here...for reverse transcriptase are packaged (Wu et al., 1997) inside of the capsid . This is useful for viral...) during step 3 (King et al., 2008) (Hu & Temin, 1990). At step 3, the double stranded DNA is integrated...plasmids γ-Retrovirus Guide References Cullen, B. R. (1992). Mechanism of action of regulatory proteins encoded..., 375–394. https://doi.org/10.1128/MR.56.3.375-394.1992. PubMed PMID: 1406488. PubMed Central PMCID: PMC372876... H., Conway, J. A., Hunter, E., & Kappes, J. C. (1997). Functional RT and IN incorporated into HIV-1 particles...Central PMCID: PMC2258930 Hu, W.-S., & Temin, H. M. (1990). Genetic consequences of packaging two RNA genomes...

  11. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...Lentiviral vector history and components In the early 1990s, researchers developed viral vector systems based...which could be collected for experimental use. In 1996, Naldini et al. transduced rat neurons in vivo with...vector technology has come a long way since the 1990s, both in terms of research impact and safety. Many...Mulligan R, Gage FH, Verma IM, Trono D. Science. 1996 Apr 12;272(5259):263-7. PubMed PMID: 8602510. 2....Mandel RJ, Nguyen M, Trono D, Naldini L. J Virol. 1998 Nov;72(11):8463-71. PubMed PMID: 9765382. PubMed...Blömer U, Takahashi M, Gage FH, Verma IM. J Virol. 1998 Oct;72(10):8150-7. PubMed PMID: 9733856. PubMed ...

  12. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...recombinase activity (Logie and Stewart 1995; Metzger et al. 1995). Visualizing single neurons in the living...optimum of 37 °C (Buchholz, Angrand, and Stewart 1998). FLPe’s performance was further improved, by adding...Buchholz, F, P O Angrand, and A. Francis Stewart. 1998. “Improved Properties of FLP Recombinase Evolved...20506501. 6. Logie, Colin, and A Francis Stewart. 1995. “Ligand-Regulated Site-Specific Recombination.”... Metzger, D, J Clifford, H Chiba, and P Chambon. 1995. “Conditional Site-Specific Recombination in Mammalian...

  13. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...Selection of Recombinant DNA by CcdB.” Biotechniques. 1996 Aug;21(2):320-3. PubMed PMID: 8862819. 2. Bahassi...retain their regulatory functions.” Mol Microbiol. 1995 Mar;15(6):1031-7. PubMed PMID: 7623659. 3. Bernard...vectors using the F plasmid ccdB killer gene”. Gene. 1994 Oct 11;148(1):71-4. PubMed PMID: 7926841. 4. Bernard...ATP-dependent DNA cleavage by gyrase.” J Mol Biol. 1993 Dec 5;234(3):534-41. PubMed PMID: 8254658. 5. Bernard... of DNA-topoisomerase II complexes.” J Mol Biol. 1992 Aug 5;226(3):735-45. PubMed PMID: 1324324. 6. Tam...

  14. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...Miyawaki et al., 1997), was designed to measure intracellular calcium and published in 1997. Since that time... efficiency, circular permutation (Baird et al., 1999) of the fluorescent protein (i.e. rearranging the...Proceedings of the National Academy of Sciences 96.20 (1999): 11241-11246. PubMed PMID: 10500161. PubMed Central...fluorescent proteins and calmodulin." Nature 388.6645 (1997): 882. PubMed PMID: 9278050. 3. Meng, Fanjie, et...

  15. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...overall replication of pSC101 (del Solar et al., 1998). If that’s not enough to keep replication in check... and blocks RepZ translation (Asano & Mizobuchi, 1998). This system keeps RepZ levels low even if plasmid...10.5772/19313 References Asano, K., & Mizobuchi, K. (1998). Copy number control of IncIalpha plasmid ColIb-P9...Ruiz-Echevarría, M. J., Espinosa, M., & Díaz-Orejas, R. (1998). Replication and control of circular bacterial ...434–464. https://doi.org/10.1128/MMBR.62.2.434-464.1998 Grimwade, J. E., Rozgaja, T. A., Gupta, R., Dyson...

  16. Five Popular Model Organisms

    Type
    Blog Post
    ... time consuming. GAL4/UAS was first described in 1993 by Norbert Perrimon’s lab and has been continuously... that express fluorescent markers (Mello et al., 1991). Genes can also be easily knocked-down through ...that contain your gene of interest (Fire et al., 1998). The discovery of RNAi in the worm actually rewarded... RNA in Caenorhabditis elegans." nature391.6669 (1998): 806. PubMed PMID: 9486653. Hesselson, Daniel, ...transforming sequences." The EMBO journal 10.12 (1991): 3959-3970. PubMed PMID: 1935914. PubMed Central...

  17. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... link with nearby nucleic acids (McInnes et al., 1990). Photobiotinylation is sometimes easier and less...specificity.” Trends in Biochemical Sciences, 24.9 (1999b): 359–363. PubMed PMID: 10470036. 3. Said, H. M....uses of photobiotin.” Methods in Enzymology 184 (1990): 588–600. PubMed PMID: 2388593 7. Holden, M. A....RNA–protein interactions.” Nucleic Acids Research, 23.18 (1995): 3792–3793. PubMed PMID: 7479014. PubMed Central...Official Journal of the Histochemistry Society, 41.12 (1993): 1751–1754.PubMed PMID: 8245422. Additional resources...

  18. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...production (Oyaas, 1989; Ozturk and Palsson, 1991; Oh, 1993) and increased yields in adenoviral (Coroadinha...butyrate." Biotechnology and bioengineering 42.5 (1993): 601-610. PubMed PMID: 18613082. 3. Oyaas, K., ...production." Biotechnology and bioengineering 37.10 (1991): 989-993. PubMed PMID: 18597326. 5. Shen, Chun ...

  19. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...applying this mixture to your tissue (Tuson, et al., 1990; Goodpaster, et al., 2013). One issue with this ...recognize the endogenous IgGs (Lu and Partridge, 1998). After washing away unbound fragments, you can ...doi.org/10.1369/0022155413511620  Lu QL, Partridge TA (1998). A New Blocking Method for Application of Murine...002215549804600813  Tuson JR, Pascoe EW, Jacob DA (1990) A novel immunohistochemical technique for demonstration...

  20. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...staining but they do not always eliminate the issue (Lu 1998). Consequently, if you find yourself in a sticky... recognize some rat primary antibodies (Erickson 1993). It is always better to choose primary antibodies...resources References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application of Murine... Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques...
Showing: 1 - 20 of 151 results