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We narrowed to 74 results for: 5-Oct

Showing: 41 - 60 of 74 results
  1. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...job where people have regular schedules. Work 8 to 5 or 7 to 4 or maybe 7 to 6 and 8 to 10 or whatever ...before you get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The house can...And, yes, your partner CAN take the kids to the doctor without your help. Your partner is equally responsible...eight-year-old daughter vehemently reminded her doctor that she was allergic to penicillin – I am much...
  2. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...implants have been reported by several groups [4, 5, 6]. These have the obvious advantage of not requiring...PMID: 23580530; PubMed Central PMCID: PMC3769938. 5. Iwai Y, et al. A simple head-mountable LED device...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...
  3. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...the desired chromosomal modification can be made in 5 days. Counter-selection with sacB has revolutionized... editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason, Lynn...This post was contributed by Jacob Lazarus, a postdoctoral researcher at Harvard. There’s an astounding...is now an attending physician. He performs post-doctoral research in the lab of Matthew Waldor at Harvard...
  4. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ... facilitate the isolation of modified cells; and (5) is compatible with the introduction of unique molecular... Find FITS plasmids here! Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard...
  5. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... Therapy Vol. 25 No 5. PubMed PMID: 28391961. PubMed Central PMCID: PMC5417792. 5. Naldini,L., 2015. Gene...amaurosis. This is the first of what I, scientists, doctors and patients hope are many more clinical successes...
  6. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...surgeries for viral injections can be found here [5, 6]. Opsin transgenic mice Transgenic mouse lines ...10.1038/nmeth.1808. PMID: 22179551; PMCID: PMC4165888. 5. Harris JA, et al. Adeno-associated viral vectors ...activation and silencing. Nature neuroscience. 2012;15(5):793-802. doi: 10.1038/nn.3078.  PMID: 22446880; PMCID...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...
  7. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...acquisition and retrieval of contextual fear memories [5].  In a modified behavioral paradigm, the authors ...primate cortex." Frontiers in systems neuroscience 5 (2011). PubMed PMID: 21811444. PubMed Central PMCID...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...
  8. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...Make a stock solution of 2.5 mM biotin (50X) because 5 mM is barely soluble at RT. Track expression and purification... GE Life Sciences) or bio-layer interferometry (Octet, Pall ForteBio). Tips for using pMal-T-Avi-His/BirA...for plasmid version 2.0? Instruments such as an Octet may require less in vivo-biotinylated protein (total...
  9. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...and to find other posts that deserve a second look. 5 Great Apps for Lab Life This very useful post was ...deposited plasmids on this trip. Here’s to hoping that October is even more awesome than September! Tyler J. Ford...
  10. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...consumption in comparison to 37% of the general public (5, 6). Similarly 90% of active research scientists believe...activity while much less of the general public agrees (5, 6). Many of the new administration’s actions, including...active research scientists (scientists who hold a doctorate degree, are currently working full-time, and have...
  11. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...Temporarily inhibiting this complex may allow time for 5′ flap exonucleases and DNA ligases to act first. PE4... optimized enzyme can be used with any of the PE2-5 approaches, sometimes referred to as PE2max, PE3max...with twin prime editing. Nature Biotechnology, 40(5), 731–740. https://doi.org/10.1038/s41587-021-01133...post was originally written by Jennifer Tsang in October 2019 and updated by Emily P. Bentley in December...
  12. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...whose plans are unknown (up to 33% from 22%), a full 5% of postdocs who were expecting a salary increase ...of Information Act (FOIA) requests for their postdoctoral salary information as of December 1st. Salary...are an important step forward in helping the postdoctoral population continue to pursue academic work,...of the scientific enterprise by reducing the postdoctoral population which keeps growing despite a lack...
  13. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...implies. These 5 organizations, according to the NSF data, account for 18% of the postdoctoral workforce in...exactly how postdoctoral salaries are changing. Transparent salary information for postdoctoral positions...institutions set their postdoctoral salary scales to the NRSA scale (Figure 21, National Postdoctoral Association...Stanford raised salary levels to $50,000, and as of October 1st the salary minimum was raised again to $51,...as having postdocs, representing ~85% of the postdoctoral workforce. Based on our communications with ...to have to get rid of postdocs; that current postdoctoral appointments may shorten in length; that postdoc...approach to biomedical research in the U.S. and postdoctoral training has not been demonstrated to be required...
  14. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...industry postdoc  Time: Academic postdocs can be long—5 years or more, especially in the biological sciences—whereas... post was contributed by Laura Desrochers, a postdoctoral scientist at AstraZeneca. Why I chose an industry...Desrochers! Laura Desrochers is currently a Postdoctoral Scientist at AstraZeneca. She has a background...blog posts here Find 9 tips for a successful postdoctoral experience Read Joanne Kamen's 8 tactics for...
  15. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...This is the fourth in a 5 part series on Management for scientists. Once you are responsible for managing...delegation tips and practices can be found at the Time Doctor Blog "The Art of Delegation: Developing This Essential...
  16. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...the life sciences end up in tenure track position 5 years after receiving their PhDs, still strive to ...research community to develop a plan to make postdoctoral pay/benefits commensurate with work training...a postdoc as “An individual who has received a doctoral degree (or equivalent) and is engaged in a temporary...image below). Slide of information gathered by Postdoctoral Associations around Boston - From Panel Session... staff scientists. This results in a glut of postdoctoral trainees who, even though they are aware that...become actionable. Additional Resources The Postdoctoral Experience Revisited. PubMed PMID: 25590106....
  17. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ... plasmids bearing one of the distal-less homeobox 5 and 6 (Dlx5/6) enhancer elements and combined it with... and constitutively expressed sgRNA targeting the 5’ end of each essential gene. Koo M, et al. Cell Systems... through the expression of transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model...Addgene and include a variety of cMYC, KLF4, SOX2 and OCT4 DNA binding domain and transcription activation ...
  18. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...will end up with a stellar letter of support.        5. Application submission  Set a calendar reminder for...Pierre! Roodolph (Roo) P. St Pierre is currently a doctoral candidate in the Chemical Biology Program at Harvard...
  19. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...Applications in Gene Therapy: An Overview.” Cells, 12(5), 785 (2023). PubMed PMID: 36899921. Resources on...Guerin and Susanna Stroik. Didem Goz Ayturk is a Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal...
Showing: 41 - 60 of 74 results