... of HEK cells with adenovirus 5 DNA was postdoc Frank Graham’s 293rd experiment, hence the nomenclature...endothelial, epithelial, or fibroblast. However, Frank Graham showed HEKs display many immature neuron-...great model of kidney cell biology, there are thankfully many other applications that they are useful ...

...control. For easier portability, all viral genes are flanked by EcoRI and BamHI restriction sites - so cloning...to mention that our FedEx guy has been the best: Thanks, Todd! As you can imagine, it’s also been quite...related plasmids and resources at Addgene* Many thanks to our guest bloggers Manon Eckhardt (left) and...

...of the De-jargonizer.   Academic Phrasebank  The Academic Phrasebank is a resource for phrases commonly...being critical, or defining terms. The phrases in Phrasebank are generic, so they need to be edited to suit...

...s flattering to ask.  Show appreciation. Send a thank you note or gift to someone who helped you succeed...any way. Don’t believe people who tell you that thank you isn’t necessary. I once got a box of 6 perfect...helped someone get a job interview. Even just a thank you email would have been enough, but I’ll never...

...reference protein) under identical conditions, a ranking of the practical brightness can be made (Goedhart...repeating this with another fluorescent protein, a ranking of practical brightness can be made. This strategy...different emission filters) or different set-ups. Many thanks to our guest bloggers Joachim Goedhard and Marieke...

...heterogeneous when constrained by sequence identities in flanking sequence (“microhomologies”). NHEJ doesn’t obligatorily...canonical Cas9 approach. A pair of CRISPR guides that flank regions of hundreds of base pairs or more can simultaneously...Christina Mork contributed to the update of this post. Thank you to our guest bloggers!   David Wyatt is a graduate...

...the Max Planck Institute for Brain Research in Frankfurt am Main, Germany. She presented a novel technology...to be invited to learn from them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing...endogenous proteins in any cell type. Exciting! Many thanks to our guest blogger, Eugenia Rojas! Eugenia Rojas...

...several human and murine CRISPR pooled libraries, but thanks to a deposit from the laboratory of Dr. Ji-Long...constructs, and complete screening in just 3 days? Thanks to Tyler Jacks’ lab, this 3-day goal is is now ...vectors contain uracil-excision based (USER) sites, flanked by ADH1 and CYC terminators for easy cloning of...revolutionized genome editing in multiple organisms thanks to its simple, easily modifiable RNA-guided targeting...difficult to quantify interaction strengths. Erich Wanker’s group at the Max-Delbrueck Center for Molecular...

... the interview. Greet each interviewer and then thank them once the interview is complete. Remember, your...you, so be engaged with their conversation. Send thank you emails shortly after the interview to the hiring...took during your interviews to help craft these thank you's. Interviewing remotely Remote interviews are...

...sequencing for quality control for over ten years. Now, thanks to a generous donation by the Hirsh family, we ...completes, we begin our assembly process. Again, thanks to our partners at seqWell we make use of a pipeline...labs often provide insert sequences or annotated Genbank files that are useful for more complicated plasmids...

... don’t follow through on their promises, Wet-blanket negativist, who can bring down the morale of a ...hostile-aggressives, complainers, know-it all experts, wet-blanket negativists, and micromanagers), while other supervisors...book “How To Win Friends and Influence People.” Thank you to our guest blogger!  Dora Farkas received ...

...particular structures (Table 1). Each construct was flanked with a CMV promoter (to drive high-level expression... contained in individual plasmids and are each flanked by the same multiple cloning site (MCS). Modules...original pattern of the MCS is regenerated at the flanks of each ligation product, which is the basis for...

...mini-transposon), which contains the DNA cargo of interest flanked by ~50-150 bp transposon end sequences (R and L...recognized during transposition, genetic cargos must be flanked by the L and R ends - thus, scarless insertions...CRISPR-transposons has yet to be fully realized. Many thanks to our guest blogger Leo Vo! Leo Vo is currently...

...easily be attached with a hose to a nitrogen gas tank with a pressure regulator. Other methods for physical... new putative host cell-binding proteins! Many thanks to our guest blogger Jessica Sacher! Jessica Sacher...Twitter @JessicaSacher.   References 1. Nobrega, Franklin L., et al. "Revisiting phage therapy: new applications...

...sequence to flank a transgene (homologous pairs) or used different scrambled sequences to flank the transgenes...orientation and one of the unused (loxp) sites will be flanked by two lox sites now in the same orientation. Next...

...the promoter to prevent its expression, and is flanked (“floxed”) by two sets of incompatible recognition...molecular mechanisms of the brain in vivo. Many thanks to our guest blogger Alessia Armezzani from genOway...233.39 (1971): 149. PubMed PMID: 4940442. Schnütgen, Frank, et al. "A directional strategy for monitoring Cre-mediated...

...spreadsheet to [email protected] along with your GenBank files, plasmid sequences, and/or maps. Add plasmids...utilize your plasmids (these can be protocols, GenBank files, etc). We can accept sequence files in any...to include the total size of the insert and the GenBank ID associated with your insert. Pro Tip! If the...

...note: in this table, reprogramming efficiencies ranking as low, medium or high is based on the reprogramming...for deletion of the inserted transgenes that are flanked by loxP sites. While there is only a small loxP...donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences, which the PB transposase...

...decorations, our holiday spirit remains. We would like to thank everyone who participated in our annual #Deckthelab... ever! & they totally succeed! WOW! #deckthelab Thank you to everyone who participated and congratulations...

...first time, and thinking about the future. Many thanks to our guests,Olivia Ho-Shing, Mark Springel, Arooba...Ahmed, Jiachen Lee, and Jillian Parker! Special thanks to our sound engineer/producer, Eddy Page! Edward...