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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 149 results for: CO

Showing: 41 - 60 of 149 results

  1. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...workshop. Specifically, Jo, Brook, and Angela are co-leading "Making a Greater Impact Using Clear and ...

  2. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    .... In this method, the major capsid protein VP1 is co-expressed with a fusion protein containing VP2C and... research, which required protein compartments to co-localize multiple enzymes in a reaction pathway, ...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ... firefly luciferase activity in the Co-IP compared of a control Co-IP indicates stronger interaction. ...catalytic domain from DNA methyltransferase, DNMT3A. Co-expression of this modified Cas9 along with a gRNA... new method called DULIP (dual luminescence-based co-immunoprecipitation) to overcome the limitations ...Firefly luciferase. The bait and prey plasmids are co-transfected and proteins are immunoprecipitated from...tet-inducible OsTIR1 plasmid is also available). Second, they co-transfected short-arm donor vectors containing mAID..., and Cas9 activator expressing plasmids that can co-express 1-8 gRNAs. Ordon et al., 2016 tested the ...

  5. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...because any potential employers, collaborators, or co-workers want to know that you are a well-rounded ...Maybe you demonstrated empathy for a collaborator or co-worker and showed that you really understood their...

  7. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ... filaments with mGold2. Top: TIRF-SIM imaging of COS-7 cells expressing LifeAct fused to mVenus, mGold2s... plant model Arabidopsis (Weiss et al., 2025). By co-delivering TRV1 with TRV2 that encodes ISYmu1 and...

  8. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ... DBDs (see Figure 2a) (4). These fusions are then co-expressed in a yeast strain that also contains a ...your proteins are not post-translationally modified, co-expression of the enzyme responsible for the modification...

  9. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ... protein (e.g., GFP or luciferase) that is either co-transfected with the test plasmid or transfected ...how much plasmid DNA to use for any individual or co- transfection, should be determined empirically.  ...

  10. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...1.1 Affinity Frequently used for western blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere...1.2 Affinity Frequently used for western blots, IP, co-IP, IF, flow -cytometry, but rarely used for purification...

  14. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...enhances RNA-guided methylcytosine editing by TET1 by co-delivering TET1 and protein factors for linking methylcytosine...

  17. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...for gene delivery. In 2018, he interviewed Addgene co-founder Melina Fan and former Associate Director ...
Showing: 41 - 60 of 149 results