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We narrowed to 148 results for: Coli

Showing: 41 - 60 of 148 results

  1. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...elements required for plasmid maintenance within an E. coli cell, but vectors can be widely utilized across ...necessary components to allow shuttling between E. coli and yeast cells. To be useful in the lab, the vectors...Can this be used for auxotrophic selection in E. coli? Additional considerations HIS3 L-hisitidine no...

  2. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...transform the PCR products into E. coli. Ligases within the E. coli then fuse together the PCR products...inexpensive DpnI enzyme. Otherwise you let your E. coli do the work for you. Traditional restriction enzyme...

  3. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ... other organisms. If you’ve ever wondered why E. coli is safe to use in the lab but also causes outbreaks..., Gateway cloning relies on genes found on an E. coli fertility plasmid that also encodes a toxin, CcdB...to that toxin, CcdA. Knowing which strains of E. coli contain this F plasmid is critical for successful...Some bacteria possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria in the ...plasmids carrying genes for bacterial toxins like colicin typically also carry the necessary resistance genes...survive their own secretions. Pro tip! Historically, colicin-producing plasmids have been referred to as “Col...

  4. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ... plasmid DNA extraction and purification from E. coli is the presence of the protein endonuclease I encoded...encoded by the gene endA. Nowadays, the most common E. coli strains used for plasmid amplification are endA ...

  5. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...library is transformed into E. coli for amplification. Transformed E. coli are allowed to grow on plates...is similar to amplifying a plasmid: transform E. coli with the pooled library DNA, allow the bacteria ...

  6. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...vectors are usually designed to shuttle between E. coli and Agrobacterium tumefaciens, a plant-infectious...species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is especially common in yeast...shuttling between. For example, if you are using E. coli simply for easy cloning and quick DNA production...

  7. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...different strains of E. coli that can be used for transformation. The preferred E. coli strains for storage... has been received and stored, it is put into E. coli strains that have been treated to easily take in...

  8. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...chromatography. Purification of His fusion proteins from E. coli is generally more straightforward and of a higher...a Solubility Enhancer. In P. E. Vaillancourt, E. coli Gene Expression Protocols (Vol. 205, pp. 99–118)...more!   Resources and References   References Coligan, J. E., Dunn, B. M., Speicher, D. W., & Wingfield...

  9. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...transformed directly into chemically competent E. coli and transformants selected with the appropriate ...have been isolated from methylation competent E. coli for this procedure to work properly. Directly transform...

  10. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    .... Max Juchheim in this post. 3. Plasmids 101: E. coli Strains for Protein Production Addgenie Julian Taylor-Parker...explains why you might want to use a specialized E. coli strain for protein production and gives a quick ...

  11. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...acids E. coli and Mammalian Cells pEVOL-pAzF synthetase for p-azido-l-phenylalanine E. coli pEVOL-...proven most effective only in a few cell types (E. coli and Xenopus oocytes, mainly). Nevertheless, tRNA...pEVOL-pBpF synthetase for p-benzoyl-l-phenylalanine E. coli pAcBac1.tR4-MbPyl pyrrolysyl-tRNA synthetase Mammalian...

  12. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...Burke Lab (Roux et al., 2012). BioID utilizes the E. coli biotin ligase, BirA*, with a site mutation (R11G...DNA-binding domain (233 amino acids) found in the E. coli version. It also contains a single mutation (R40G... noise ratio 30-fold compared to the standard E. coli BirA* mutant. RapID consists of this mutant BirA...

  13. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...gram negative bacteria include salmonella and E. coli. Because of their thicker peptidoglycan layer, gram... Blue-white screeningPlasmids 101: Common lab E. Coli strains  ...

  14. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...reaction into chemically or electro-competent E. coli - excess salt causes arcing during electroporation...vaccinia virus DNA topoisomerase I in Escherichia coli is sequence specific."  Proc Natl Acad Sci U S A...

  15. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...overexpression of various metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques ...metabolic engineering realm of S. cerevisiae and E. coli. The utility of simultaneous and multidirectional...

  16. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ... that’s easier to express in the cytoplasm of E. coli, although this is not possible for all scFvs.   ...single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of Sciences...Sivaccumar JP, Ruvo M (2020) Evolution of Escherichia coli Expression System in Producing Antibody Recombinant...

  17. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...polymerase I (PolI3M) is capable of mutagenesis in E. Coli, but this approach requires special bacterial growth...mutations per nucleotide per generation of wild-type E. coli, while only increasing the standard mutation rate...

  20. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...nonmethylated PCR products are then transformed into E. coli to undergo nick repair. This method can work well...relying on in vitro assembly, they could make the E. coli do the work by using the principles of recombineering...
Showing: 41 - 60 of 148 results