...elements (often linked in groups of seven as a Tet Response Element (TRE)), allowing transcription to proceed...to the promoter and turning transcription OFF. Despite their bacterial origins, Tet systems function well...transactivators GALQF and LexAQF, which activate their respective UAS and lexAop sequences but are also regulated...expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S A. 1992 Jun...Valle Rodriguez, Alberto, Dominic Didiano & Claude Desplan. “Power tools for gene expression and clonal analysis...

...community for years to come. We Are Here to Help Despite these challenges, Addgene remains steadfast in ..., helping teams to be successful in their work despite limited resources. These resources become even ... scientific progress depends on our collective response. Addgene stands ready to collaborate with partners... this time. Your input will directly shape our response initiatives as we stand with the scientific community...

... Addgene believe in the power of sharing, and especially in keeping the products of science accessible...clinical trial data, serve society and patients, and respect industry concerns.” 5)      In the aftermath of...journal impact factor, study topic, and the corresponding author's country of origin, were taken into ...content providers and participation from many well-respected and high-profile universities. We particularly...

...immune response against the capsids or transgenes. Sensory neurons and liver appear especially susceptible...impact of neutralizing antibodies developed in response to the first exposure to AAV9. The exception is...your prep can help reduce the risk of an immune response. Strain-specific tropism in mice Many systemic... in RNA and DNA levels (~25-fold and ~4-fold respectively) relative to AAV9 in the pigtail macaque brain..., B.E., Gradinaru V. Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nature...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature Biotechnology...

...me a full description of the tasks I would be responsible for, in comparison to the other positions listed... helped me transition easily. Training and responsibilities Through this training, I was gradually introduced...for the independent work on the QC team. I was responsible for helping other scientists on the team ensure...sequencing QC work    In addition to my responsibilities on the QC team, I was able to explore other...

... before/after application of NE (10 μM) and corresponding pixel-wise ΔF/F0 heatmaps. White insets indicate...and selective for norepinephrine and faster to respond than GRABNE. While GRABNE is blocked by alpha2-...because alpha2-AR can provide inhibitory feedback in response to norepinephrine release, and clinically-used... deactivated three times faster. This enhanced response time allows for more precise measurement of norepinephrine...

...inactive dCas9 and VP64, respectively. CibN and Cry2 form a heterodimer in response to blue light, which ... pHlash reporter protein include: H+ specific response cytoplasmic retention insensitivity to other ions...   The Gideon Dreyfuss lab has developed rapid-response luciferase (firefly P. pyralis) reporter plasmids...TCSs). TCSs are utilized by bacteria to sense and respond to their environment - typically through a histidine... kinase, which triggers phosphorylation of the response regulator and downstream transcription activation...optimizing expression of both the light sensor and the response regulator. These changes reduced system leakiness...substrate for Renilla luciferase. This approach is especially suited to complement the use of optogenetic tools...

... most scientists start out in academia, it is especially important for graduate students and postdocs ...researchers must be extreme self-starters and especially good at self-promoting—No one is going to tell...acceptance can be an issue in getting promoted (especially race and gender) Interpersonal issues can create...first step. Developing mentoring relationships, especially with peer mentors, to discuss your strengths,...

... traction.  This can carry a heavy price tag, especially if you're working on something other than software....  Your venture should continuously evolve and respond to feedback throughout its lifetime.  In this way...company) and is our money guy (CFO).  As CEO, I am responsible for internal operations and building external...discuss miLab.  Although many emails resulted in no response, I was still able to get through to a number of...

...Group of the NCI RAS Initiative, led by Dominic Esposito, has recently released a collection of 360 Ras...by restriction digest and Sanger sequencing, respectively. The speed and simplicity of GMAP allows scientists...tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many of which are available...TALEN(Sangamo)-Destination series, pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2, pCAGGS-TAL-NC2...altering the number of tandemly expressed gRNAs respectively) and 2) the size of the deletion. Smaller deletions...livestock diseases such as malaria and toxoplasmosis. Despite their importance to global health, most apicomplexan... kinases has been linked to several diseases, especially cancer. A great deal of chemical biology and ...

...immunogenic, meaning that they elicit an immune response from the body. Several factors contribute to the...a foreign antigen, triggering a humoral immune response. ADAs can inactivate the drug and increase drug...adverse immune reaction to the treatment. These responses vary depending on the person. However, in silico...to be presented by T cells to elicit an immune response. T cell epitopes are found in either the therapeutic...

...from mouse, and showed that it can alter motor responses in C. elegans. Find the split BoTN/B plasmids ...expression across the majority of cellular pathways. Despite several years of research, the mechanisms of miRNA...connection between specific genes and an immune response has been a longstanding challenge. Using shRNA...with no deleterious effects on the in vivo immune response other than what would be the anticipated effect...

... immunogenicity, meaning they induce an immune response in the host. So why use a gamma-retrovirus in...meaning it does not induce the same high immune response as retroviruses. This virus can also transduce...population. Some adenoviruses are known to cause respiratory disease across species, as well as cancer in ...been engineered to induce a lower inflammatory response within hosts. Relative to the other viruses discussed...

...novel optogenetic variant of AcrIIA4.” CASANOVA responds to blue light quickly. Within minutes of inactivating...has high signal:noise ratio, high sensitivity, responds quickly, is photostable, and is compatible with...proteins, which change in protein conformation in response to light, offers a way to spatially and temporally...photoswitchable cPYP, which changes in conformation in response to blue light (445nm). The discovery and applications...

...science or even to discuss potential solutions. Despite all the talk, not much was being done to address...practices could be an actionable way to tackle widespread irreproducibility. Luckily, there were other ...meeting by the collaborators who could participate. Despite the group not having a public website, all the ...practices could be an actionable way to address the widespread issue of irreproducibility. Use your network...

...from Elizabeth Hubbard.   Haoyi Qiu: Pain response in mechanical allodynia Haoyi Qiu is a M.Sc. ...vivo firing frequency of PV neurons affect the response to light touch in mechanical allodynia. ...experimental approach. (B) CR-Cre mice demonstrate pain response due to blue light stimulation of dual virus-expressing...virus-expressing CR neurons. (C) Duration of painful responses in the left and right paw due to blue light activation...

...there’s no guarantee you’ll get a response on the first try (or any response at all). And while this method...free, you can always reach out to the PI or corresponding author of the paper it’s published in. That’... amount of money in reagents and consumables, especially for things like plates and gels, which either...

... of p53 tetramerization in response to DNA damage controls the p53 response. Gaglia G, Lahav G. Mol Syst...interact in cells and produce background signal (especially for some older split-GFP constructs or certain...functionality or binding of your target proteins, especially if the termini are important for their interaction...

...editing these cells to avoid a negative immune response (e.g., graft vs. host) as well as the introduction...allogenic therapies to prevent a graft vs. host response. Off-target effects are also important safety ... considerations for all CRISPR therapies, but especially concerning when using a systemic approach. Regarding... will not work in a meaningful way. Delivery, especially for systemic approaches, has its own unique set...

...held on Friday 16th and Saturday 17th September respectively. Join us to discuss advocacy efforts toward ...for students interested in leading the process despite the recent advance. Similarly, grad students throughout...currently compiling a database of institutional responses to the FLSA ruling. Similarly, from purely a salary...ways. Workshops will be run by experts in their respective fields: David Cameron, PhD - Director of Media...