...very confusing for scientists writing research articles. What should you take into account when writing...funders of your research may stipulate that your article must be published in an open access journal or ...whether they agree with the conclusions of the article. These initiatives are another way in which journals...report are permanently attached to the published article. In addition, authors and readers are able to comment..., the authors can publish a new version of the article, while the first version and the referee reports...

...from formal mechanisms like publishing a research article, presenting a conference poster or talk, or submitting...avoid many of these issues, by sharing their work publicly and immediately once the manuscript is drafted...somewhat meaningless) practice of listing “Submitted” articles in their CV or grant applications. A number of...source or were deposited when the now-published article was a preprint. Depositing plasmids that were used... as sharing those plasmids alongside a journal article, but it makes the research materials available ...

... viral shRNA expression vector and the vector particles are delivered into the brain region of interest...vectors are used for intracerebral injections. AAV particles are smaller and may be preferable if wider diffusion...via injection into the liquor of the cerebral ventricles (ICV, intracerebroventricular) to minimize tissue... titer: The concentration of infectious viral particles must be high enough to maximize possible outcomes...injection speed has to be slow to allow the viral particles to diffuse away from the injection site, and also...

...during mitosis, support transport of organelles and vesicles, and more. Although several strategies are available... demonstrated its use to probe cell mechanics, vesicle transport, and cell division, but given the long...Aljiboury et al., 2023). Rab family proteins support vesicle trafficking and organelle dynamics, but with dozens...Figure 3: Optogenetic inhibition of Rab protein vesicle trafficking via CRY2-CIB1 clustering. Image adapted... the plasma membrane or lysosomes (the earlier article also provided CIB constructs for targeting Golgi...

...repository through our hot plasmids articles. These brief articles provide a synopsis of a plasmid or ...functions and applications. We hope that these articles make it easier for you to find and use the plasmids...glia, have been successfully targeted via viral particles, an effective approach to target specific neuronal...HF-BE3 protein for delivery in ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse...localize TRF2 to telomeres. The plasmids used in this article have been deposited with Addgene and include MYC...

...10 Hot Plasmid articles with each of our newsletters. You can find all of these articles on the Hot Plasmids...access these snippets here or from their respective articles on the Hot Plasmid webpage. We hope you enjoy ...Our Newsletter Check Out All of Our Hot Plasmids Articles Browse the Repository  ...

... lab experiences, findings, and experiments as articles written for general audiences. So far, we are ...feedbacks from our non-scientist readers about how our articles have helped them understand different topics of...launch an e-magazine this year which would feature articles, posts, and sci-art that would ultimately give...Addictive Brain. A contribution can either be an article, a short post, a video, or a piece of sci-art.  ...

...review articles, such as: Sander JD & Joung JK, Nature Biotech, 2 March 2014. Or browse the articles related...Short Palindromic Repeats, in Pubmed returned 728 articles (3/12/2014). With so many options for CRISPR plasmid...answers on the fly. But just like the growing PubMed article list, the Genome Engineering Using CRISPR/Cas Systems...

...of AAV2 this is thought to be due to the viral particle’s affinity for heparan sulfate proteoglycans on... into the cell culture supernatant, the viral particles associate with the cell membrane and are taken... we aimed to prevent the uptake of AAV2 viral particles by our packaging cells by treating the cells with...fluorescent dye and transduction of GFP-expressing AAV particles we did not observe any difference between untreated...

...appropriate factors displayed on the outside of the particle to interact with the host. This is the role of...order. This will all be ssDNA within the viral particle. The ITRs base pair with each other to allow DNA...capsid protein that helps with virion assembly and particle release from late endosomes when AAV is trafficked...proteins required for packaging of the virus into particles. E2A/E2B are responsible for viral replication...

...of new particles. With subsequent cell divisions, the fraction of cells containing the particle exponentially...assembly of fluorescent proteins into one bright particle that segregates into one cell during cell division...agglomeration of fluorescent proteins into one particle within the cell, the lab fused the red fluorescent...

... and secondary antibodies coupled to nanogold particles, that allow a high-spatial resolution of the protein...monkey neurons, the large majority of the gold particles were in the cytoplasm of dendrites, and only a...and green arrows point, respectively, to gold particles localized in the cytoplasm or bound to the plasma... protein fusions, check out Joachim Goedhart’s article on the Addgene blog: Fluorescent Proteins 101: ...

... will highlight four tools for finding journal articles related to your actual interests while also staying...publication date, a relevance score, and type of article. Now, search results display a bar graph of the...PubMed entry are great places to look for more articles on your topic of interest.  Best uses Accessing... including a bot for biosensor papers, microbe articles, CRISPR papers, and more. Saving papers There...

...expression or localization in cells, or even single-particle tracking of the bound complex. There are so many...EBFP2(1-10) can be used with GFP(11)). Some of the articles linked below only provide one fragment of the ...Protein Tags Browse the table below, and click the Article link to find all the plasmids from the associated...associated paper:   Color Base FP Description Article PI Blue EBFP2 EBFP2(1-10) and Capri(1-10) for ...

...production of viral particles, and transduction of a target cell line with those particles. Since the Env,...Nervous about handling viruses? That’s okay! In this article we will discuss general viral safety considerations...you’ll need to take extra steps to ensure that particles are not sprayed into the air. For lentivirus and...

...lentivirus, AAV requires a higher number of viral particles for efficient gene transfer. I figure it’s better...alone. If yield is important, or if the viral particles of the AAV serotype you’re working with are primarily...ethanol and dry ice bath to release the virus particles from the cells. Cell lysates are then treated ...quantifies the number of genome-containing viral particles that are present in an AAV prep. AAV samples are...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid... polyomavirus virus-like particles (MPyV VLPs ). This artificial nanoparticle can protect proteins from...

...suppressors to the accelerator and brake pedal of a vehicle, respectively. In the case of a mutation that turns...oncogene), this is analogous to the accelerator of a vehicle being stuck down. Similarly, a mutation that turns...the same result – uncontrolled movement of the vehicle or, in the case of cancer, uncontrolled cell proliferation...non-cancerous cells, such as those within hair follicles and lining the digestive system, causing discomfort...

...ribonucleoprotein delivery by lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells...’s lab developed a lentiviral capsid-based bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins...binding protein for packaging into a lentivirus-like particle. Read the paper in Nucleic Acids Research Find...

...Additionally, a concurrent Nature Biotechnology article describes the development of a mouse lentiviral...genomic screens Using lentivirus as the delivery vehicle, Neville Sanjana, also of the Broad Institute, ...M, Yusa K. Nature Biotechnology. 2013 Dec 23. (Article). Read more about CRISPRs: Using CRISPR/Cas to...