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Showing: 41 - 60 of 362 results

  1. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ... system.  As the number of CRISPR reagents continues to grow, so too will the number of reagents available... cannot be modified using wild-type SpCas9. The number of off-target cleavage events for the VQR and VRER... and will continue to dramatically increase the number of CRISPR applications. The first non-Cas9 CRISPR...

  2. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...plots often contain other data. Some will have numbers by gated populations; this indicates the percentage...Boix et al., 2018, uses quadrants to gate and has numbers representing the percentage of cells in each gate... In a contour plot, each line contains the same number of events. Thus, contour lines that are close together... histograms from Alyamani et al., 2018, showing number of events within a single signal in a flow experiment...

  3. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ... encoded fluorescent tag. There are, however, a number of limitations that you may run into if you choose...reflected by the pKa, which is around 6.0 for GFP. This number implies that at pH 6 only 50% of the available ...available (Cranfill et al., 2016 and FPbase.org). A number of variants remain fluorescent under acidic conditions... solutions for many of the limitations, and the number of alternatives to GFP is expected to grow with...

  4. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ... from 90-160 distinct colors due to higher copy number. The name Brainbow is truly fitting for this colorful...individual neurons, the system also suffered from a number of limitations. First, the imaging of Brainbow ...expressed by a majority of neurons, limiting the number of distinct colors that could be observed in a ... of Cre-negative Brainbow mice to determine the number and type of cells in which the construct is expressed...

  5. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...with two numbers separated by a slash: the first number refers to the genome, and the second number to the...thirteen natural serotypes of AAV identified so far, numbered AAV1, AAV2, and so on. In addition, researchers...

  6. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...in your cargo. Additionally, with piggyBac™, the number of integration copies can be tightly controlled...of transposase to transposon result in a greater number of integrations per cell and a super-piggyBacTM...super-piggyBacTM can be used to get an even greater number of integrations. It should be noted, however, that piggyBac...or a dominant-negative mutant. Reducing the copy number can also mitigate the deleterious effects of expression...

  7. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...equivalent numbers of colonies on each plate (unsuccessful 3).     If you have a high number of colonies...: insert ratio” of approximately 1:3. Since the number of base pairs for each varies, it is difficult ...successful ligation. Pick 3-10 colonies depending on the number of background colonies on your control plate (the...

  8. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...reduce false positive rates and control for large numbers of comparisons. The Bonferroni does this by establishing...than or equal to the alpha value divided by the number of null hypotheses. Benjamini uses a complementary...largest p value where its rank divided by the total number of comparisons performed multiplied by the alpha...all sample comparison is needed, without a high number of samples (~5). Tukey’s test – Most conservative...

  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...individual Level 0 plasmids, or choose from a growing number of libraries containing pre-constructed standardized...utilizes a 4-module assembly system, which reduces the number of individual repeat-variable di-residue (RVD) ...hundreds of possible combinations (depending on the number of copies). This facilitates the distinction of...spatio-temporal control over expression and increases the number of species in which Brainbow may be used. This ...Max-Planck Institute of Biochemistry has developed a number of expression vectors for use with Sequence and...

  10. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...homemade competent cells will work just fine.  A number of protocols for making competent cells are available...without a heat-shock step. Because of the large number of transformations being performed at Addgene, ...your cells can be readily transformed. Compare the number of colonies obtained using your fresh batch of ...

  11. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...institutions in Europe. In the last four years alone, the number of plasmids distributed to Europe has doubled going...9,500 in 2010 to more than 20,000 in 2013. And the number of plasmids requested by scientists based in Europe... scientists in Europe. Despite these fantastic numbers, a lot of European scientists and institutions ...

  12. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ..., such as U6 or EF1α promoters, has limited the number of gRNAs that can be packaged into viral vectors...high efficiencies, which is scalable for a higher number of MPE. Therapeutic potential  To explore the therapeutic...screenings that would perturb the unprecedented number of genes with a single viral vector.   ...

  13. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...library: in other words, 100-1000x more reads than number of plasmids in the library. Analyzing the reads... the pre-amplification DNA, you’ll compare the numbers before and after. However, because there are hundreds... file from Feng Zhang’s laboratory. There are a number of other analysis scripts available, some associated...

  14. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...you’re prepping for your experiment - there are a number of outside factors that need to be considered as...thing you should do is record the manufacturer, lot number, and expiration date somewhere you can easily check...with a simple kit, and is readily available on any number of secondary antibodies. And look - if it ain’t...

  15. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...sequences (NG, GAA, and GT), greatly increasing the number of genomic loci that can be targeted (Hu et al....activity, relative to eSpCas9 and SpCas9-HF across a number of different sites. Using a combined random and...both specificity and sensitivity across a large number of loci and gRNA lengths. Recent alternatives to...

  16. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...Naming conventions Give every experiment a number: Numbers are a short, easy way to name files so that...know which files go together. I also prefer the number system to using dates as labels, since I often ...

  17. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...world of science publishing, and an ever increasing number of journals and publishers to choose from, it gets...needing a subscription to the journal. An increasing number of funding bodies insist that the research data...information on lab resources, such as catalogue numbers and manufacturers’ details for antibodies, plasmids...

  18. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ... recognize and bind proteins. They’re used in a number of research and clinical applications. Most of ...source, may not be readily available for a large number of targets, and can require specific conjugates...libraries, and high specificity has been reported for a number of targets. Due to their size and lack of disulfide...

  19. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...too costly and time intensive to process a large number of microbiome samples. To enable large-scale, longitudinal...Toolkit by replacing the backbone vector’s high-copy number bacterial ColE1 origin with the RSF1010 origin,...particle exponentially decreases over time and the number of elapsed generations can be determined. The lab...

  20. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...applications. A number of organizations (such as ASAPbio) advocate for preprints, and a growing number of funding...is valuable, modern scientific publishing has a number of drawbacks, especially the increasingly growing...
Showing: 41 - 60 of 362 results