We narrowed to 222 results for: PORA

...the Cre recombinase, are used to spatially and temporally control transgene expression. Lox sites are directional...sites lets researchers control the spatial and temporal activation of a transgene. Figure 2: FLEX...

...salt causes arcing during electroporation which would cause the electroporation to fail). When incubating...

...visits an institution to perform research there temporarily. The length of stay could be from a month to ...own. This will include, but is not limited to temporary housing, transportation, living expenses, and ...

...proteins.  For computer-savvy users looking to incorporate FPbase data in their own projects and applications... and elaboration.  Current projects include incorporation of biosensors, protein evolution lineages, 2...

...when performing amplifications, and we use electroporation to ensure a good transformation efficiency....library, Addgene usually does four to eight electroporation reactions using a total of ~400ng of library...

...your gRNA. Pro tip: modifications are typically incorporated at the termini of the crRNA molecule (usually...Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation...

...permanently integrate into the host genome or temporarily express whatever it encodes. This ability to ...straightforward across applications, while electroporation of a human, for example, isn’t a reasonable...

...nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop...size of the backbone, transformations require electroporation. References & Further Reading: For more ...

...): These are considered low copy vectors and incorporate part of an ARS along with part of a centromere...nutrient, the host cells will die unless they have incorporated the plasmid carrying the required gene.  The...

...introduce modifications at the DNA level in a temporally and locally defined manner. This allows scientists...injection Viral vectors allow a tight spatial and temporal control of gene expression manipulation. They ...

...system allows scientists to both spatially and temporally knock-down proteins of interest.  Plasmids for...intersectional strategy using a split intein for refined spatiotemporal transgene control in Caenorhabditis elegans...

...ncRNA localization and function, but directly incorporating very long ncRNAs is impractical. Instead, tags...nuclease-dead Cas9 protein (dCas9) and a sgRNA to temporarily block the transcription machinery of a target...

...upon these original tools that allows for both spatiotemporal protein degradation and quantification of this...expressed under a desired promoter with a specific spatiotemporal expression pattern and an auxin inducible degron...

...interest. However, FACS is unable to spatially and temporally target single cells. Now, Francois St-Pierre’...way to isolate individual cells with unique spatiotemporal phenotypes from large heterogeneous cultures...

...viral Rev gene from the packaging plasmid and incorporating it into a fourth plasmid. In addition, the promoter...third generation packaging systems, this system incorporates the Tet-off transcriptional activation system...

...and the G protein (supplied in trans) will be incorporated into the viral envelope (but not the viral genome...B. Retrograde monosynaptic tracing reveals the temporal evolution of inputs onto new neurons in the adult...

...(1, 3). The gRNA can be further modified by incorporation of an RNA hairpin, such as the MS2 hairpin, ...with nuclease-competent Cas9. An RNA hairpin incorporated into the truncated gRNA recruits a transcriptional...

...delivered to target cells using microinjection or electroporation. Transient expression of CRISPR components,...Easy Easy Easy Physical transfection   Electroporation / Nucleofection Microinjection Cells Many...

... Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the... are available, but scientists would need to incorporate their recognition sites into their protein tag...

...tumefaciens, a plant-infectious bacteria that can incorporate foreign DNA into plant genomes. Eukaryotes are...stable transfection, DNA from the plasmid is incorporated into the host cell’s genome, so no special sequence...