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We narrowed to 468 results for: SEC

Showing: 41 - 60 of 468 results

  1. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...species-specific secondary antibody is used to visualize the proteins. In this set-up, multiple secondary antibody...denaturing. In a native western, the protein’s secondary and tertiary structures remain intact and the ..., consider reviewing the materials and methods sections of those publications.  Finally, always verify...Visualizing your protein of interest with the help of secondary antibodies In order to visualize proteins on the...

  2. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...and the pro’s and con’s of working in different sectors. Luckily, I have worked for a variety of different...another major factor held me back; I do not have a second language. During the recession there were many ...many qualified scientists with experience and a second language in Spanish or French, and so they were ideal...courses were interesting and luckily genetics is a sector which has been rapidly growing. These factors left...allowed me to try out working in many different sectors and to discover for myself what potential job opportunities...

  3. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ...down the page to the materials and methods section. This section will vary slightly depending on the application...Figure 4: A screenshot of the materials and methods section for the data report shown in Figure 3.   At the...the end of the report, you’ll find a results section, which will tell you if the antibody worked under ...

  4. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...dilution range from 1:1,000–1:100,000. Secondary antibodies For secondary antibodies, the recommended dilution...recommendations, and if needed, optimize your secondary antibody concentration by running a protein gradient...issues, try running a reagent gradient with your secondary antibody to see if lower concentrations reduce...antibody* Run a reagent gradient with the secondary antibody* Inefficient blocking Check antibody...primary antibody* Run a reagent gradient for secondary antibody* Antibody not completely binding ...

  5. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...which recognizes your protein of interest, and a secondary antibody, which recognizes the primary antibody... protein on the membrane. Then, addition of a secondary antibody allows the protein to be detected by ...takes place in a 96-well plate, where primary and secondary antibodies are used to capture the protein of ...conjugated to a fluorophore, to avoid the use of secondary antibodies in this experiment - are incubated ...then visualized using a fluorophore-conjugated secondary antibody or Fab. Image from Harden et al., 2014...

  6. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ... the class, the moderator discussed individual sections of a paper, the abstract, figures, introduction...assignments, which consisted of reading that class’s section of a manuscript and critiquing it, were not too... manuscripts and discuss changes to particular sections. The focus groups allowed us to try out techniques...produce the figure, its legend, and write a results section for that figure. Writing a scientific manuscript...

  7. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ...During this second read, I pay more attention to details and use a checklist for individual sections of the...Detailed assessment After this initial assessment, the second read of the paper is much more detailed. I prefer...the paper (see figures). After finishing my section-specific comments, I re-read what I wrote about the...

  8. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ...plasmid through the “Articles Citing this Plasmid” section. Having IDs linked to these metadata and publications... Addgene plasmid in your materials and methods section have been updated to include the option of citing... will find that the “How to cite this plasmid” section now lists the multiple PIDs separated by semi-colons...cite using the PIDs in the material and methods section of your publication. You should carefully read ...

  9. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...incubating the tubes in an upright position for 30 seconds, and then recapping the tubes prior to thaw. We...to dry ice. Does venting help? We found that 30 seconds of venting attenuated the pH change caused by storage... storage on dry ice (Fig. 3). However, 30 seconds was not sufficient to prevent any pH change, as we still...recommend both venting the tube for at least 30 seconds prior to thaw, and also using the thawed virus ...

  10. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...during a secondary immune response, which is when the immune system encounters  an antigen for a second or ...s the second most common antibody after IgG; but it's the most prevalent antibody in secretions (e.g. ...

  11. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ... figures in the results section. As you’re sorting through the results section, this is where the authors...trying to replicate an experiment, the methods section probably won’t get much attention reading through...appropriate controls? Refer back to the methods section for finer points of the protocols. Are there any...

  12. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ..., these issues which will continue to shape the sector throughout their careers (McDowell et al, F1000Research... by many institutions to set postdoc wages. The second event of note is a ruling in August by the National...funding and funding mechanisms require an engaged sector with effective advocacy skills. Advocating for ...interested in communicating science and in the intersection between science and public policy. Follow David...

  13. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...phage-assisted evolution, generating xCas9 2.0-2.6. This second round of evolution included continuous outflow ...GAT, and CAA PAMs, while xCas9 3.6 displayed the second best PAM range. Characterizing xCas9 in mammalian...efficiency fits the previous observation that NGA is a secondary PAM for SpCas9. Hu et al. performed GUIDE-seq ...know how xCas9 is working for you in the comments section! References Hu, Johnny H., et al. "Evolved Cas9...

  14. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...preps from the Deisseroth Lab INTRSECT project. We now have over 40 INTRSECT 2.0 project vectors available...light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool captures rare... labeling. Now available in serotype AAV1! New INTRSECT viral preps available! We are expanding our inventory...

  15. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...how to get started on fly work in this post. The second post will detail a major tool used by Drosophila...genome. They have four chromosomes: the X (and Y), second, third, and fourth chromosomes. Also, the fourth...generally do not manipulate them. Essentially, the X, second, and third chromosomes are the ones you’ll work...dominant phenotypes visible on the flies. For example, second chromosome balancers often have the curly O (CyO...

  16. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...slowly mature until they predominantly fluoresce a second color. This change is usually hypothesized to be...control gross changes in gene expression as new body sections are formed. In synthetic biology, FP timers might... and creative way? Let us know in the comments section below! References 1. Terskikh, Alexey, et al....differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells." Nature communications 7 (2016...

  17. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...methods and plasmids described in the preceding sections are simple ways of knocking out a non-essential...recombination – the first removes the selection marker, the second the floxed exon.   After recombination has...this exon (and hence knock out the gene) through a second round of Cre recombination.  The future of knockout...very good at making knockouts, knocking in large sections of DNA can be more difficult. Addgene depositors...

  18. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...genome engineering? How does CRISPR work? This section was essentially background slides getting everyone... What can CRISPR do? I spent more time on this section of the presentation because, in my opinion, it ...fewer examples and think of a way to make this section more interactive. If you can think of a good analogy...use CRISPR? How shouldn’t we use it? This last section was arguably the most important, and it was a little...

  19. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...that using Fab fragment secondaries, rather than traditional full IgG secondaries, can improve signal strength...and there’s just not much to be done about that.  Second, expansion introduces distortions. The goal in ...expansion microscopy using fluorescent Fab fragment secondary antibodies. Methodsx 9:101796. https://doi.org...

  20. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...Waldron While IHC protocols typically call for thin sections of tissue, the Wu Lab is working to create a three-dimensional...expression in the adult mouse brain using thick-section and whole-mount labeling techniques. The Trimmer...of Anti-PSD-95 [K28/43R] under the Application section of the Addgene antibody page, and learn more about... Anti-PSD-95 [K28/43R] (70 µm mounted sagittal section). Credit: Zhuhao Wu, Weill Cornell Medicine.  ...
Showing: 41 - 60 of 468 results