We narrowed to 58 results for: USP
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TypeBlog PostPublishedJuly 1, 2025, 1:15 p.m....potentially impacting downstream experiments. The usual suspects, such as proteins, phenol, or guanidine salts,...
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DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....Finally, DNA is precipitated with alcohol and resuspended in TE buffer. After this, the DNA is ready for... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m.... their initial findings. The team originally suspected that this new Cas7-11 might be phylogenetically... -
An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m....won’t tumble out of the sink and on to a poor unsuspecting pet. The software equivalent to this would ... -
Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m.... pellets containing viral particles are then resuspended in PBS, aliquoted, frozen, and stored at -80 ... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m.... allows for targeted modification of known or suspected genes that regulate a desired phenotype. In fact... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog PostPublishedMay 18, 2018, 7:32 p.m....experiments with too few replicates leading to suspect statistical significance (you can read more about... -
A Control for All Seasons
TypeBlog PostPublishedOct. 5, 2023, 1:15 p.m....interaction. When non-specific interactions are suspected, use a preadsorption control to confirm. In this... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m.... your membranes with PBST to remove salt, then suspend a freshly harvested dose of cells (preferably in... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog PostPublishedOct. 8, 2020, 1:15 p.m....you are reading this article then you probably suspect mycoplasma contamination in your cell culture or... -
Important Considerations When Using AAVs
TypeBlog PostPublishedJune 13, 2017, 2:30 p.m....should still be taken when using them. There are suspected cases of insertional mutagenesis in humans (1)... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....mechanism of type IV systems is not known, but it’s suspected that they don’t carry out the same types of adaptive... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m....integration modules, and many more of the usual suspects. The Multiplex Yeast Toolkit builds on the YTK... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m....transfection, add csRNP and ssODN to the cell suspension, transfect and let cells recover for 2-5 days.Note... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog PostPublishedMarch 25, 2025, 1:15 p.m....converts them back to Cs. In this case, the team suspected that the BER process was excising the incorrect... -
Deep Dive: qPCR
TypeBlog PostPublishedSept. 29, 2022, 1:15 p.m....slope generated should be less than 0.1. If you suspect that amplification efficacy differences may be ... -
Troubleshooting and Optimizing a Western Blot
TypeBlog PostPublishedSept. 17, 2024, 1:15 p.m....lengthy incubation (30–60 minutes) at 37 °C, if you suspect aggregation in your prep. If your proteins are ... -
27 Hot Plasmids from 2016
TypeBlog PostPublishedDec. 22, 2016, 3:03 p.m....the authors were able to identify 7 previously unsuspected genes with known roles in ER-associated degradation...