We narrowed to 153 results for: cycs

... of XFPs in the original method, we used the Tetracycline trans-activator system to boost the expression...expression levels, and we named the system Tetbow (Tetracycline trans-activator Brainbow) (Sakaguchi et al.,...

...experiment. Dr. Cohen and colleagues treated a tetracycline resistant plasmid, pSC101, and a newly developed...plasmids and new cloning vectors such as pBR322, pACYC, and pUC were developed to provide higher copy number...

... Last, Bernach will simultaneously monitor cell cycle and growth rate using TIMER and an unstable fluorescent...under the control of promoters that control cell cycle gene expression.  Additional Resources on the ...

...reporter mRNAs. Using translation inhibitors cycloheximide and puromycin, Halstead et al. verified that...effects of 5’ UTR variants in translation of cell cycle protein Emi1, showing that ~80% of transcripts with...

...you’re working with them, but avoid freeze-thaw cycles, as they’ll degrade your antibody.  At some point...been left out, gone through a freeze-thaw-freeze cycle before, or otherwise been subject to mishandling...

...for infection of quiescent cells or infrequently cycling cells but is fine for experiments with immortalized...integration to express its cargo, in fact its viral life cycle does not include genomic integration. While the ...

...shRNA in target cells by the addition of tetracycline/doxycycline to the grown media resulting in target...

...speed of labeling. BioID requires 6-24 hours for tetracycline induced expression of the BirA* fusion protein...Split-BioID, Schopp et al allowed 24 hours for tetracycline induced expression and biotinylation of interacting...

...cell can occur. Similar systems include tetracycline or doxycycline mediated activation of Cre to either ...

...some viral preps are very sensitive to freeze thaw cycles; the titer measured with a freshly collected lentiviral...aliquot that has been subjected to a freeze/thaw cycle for titering as this will better represent future...

... the destination construct. Reactions are often cycled between 37°C and 16°C to provide optimal reaction...terminal soak after either 37 ºC incubations or cycling.  *Assembly reaction volumes were 20 μl (1, 12 ...

...extracellular destination. At the end of their life cycle, proteins may be transported through the vesicles...proteins it is also possible to follow the life cycle of viruses and intracellular bacteria – have a look...

... to increase yield. The collection process is a cycle of collecting the media and adding fresh media every...Baculovirus replicates inside insect cells, and its lifecycle ends in cell lysis, releasing the virus to the...

... target cells and can be altered by freeze/thaw cycles (Lock et al., 2010). It is recommended that you...are quite stable and will withstand freeze/thaw cycles and dehydration (Howard & Harvey, 2017). You can...

..., one can generate constructs to visualize cell cycle dynamics, titrate inducible transgene expression...later construct (i.e.,functional cassettes are recyclable) Can only work if the restriction sites used ...

...few things you can try: Increase the number of cycles. After additional rounds of amplification, your...positive and negative droplets. No more than 50 cycles is recommended. Decrease your ramp rate. A rate...

...is tightly coupled to the bacterial host's cell cycle, maintaining a stable concentration of plasmid. ...copy number of pSC101 through a negative feedback cycle. As with DnaA, high concentrations of RepA negatively...

...generally aliquoted to reduce the number of freeze-thaw cycles needed when using the virus. A small aliquot is...lentivirus. Since viruses are sensitive to freeze/thaw cycles, we include a 1.3 mL aliquot with mostlibrary requests...

...splice acceptor. TRE General expression mRNA Tetracycline response element promoter Inducible with Tetracyline...promoter with low basal activity and several tetracycline operators. Transcription can be turned on or...

...grow and maintain in the lab, have a short life cycle of 8 to 14 days, and produce a large number of offspring...small, transparent worms that have a rapid life cycle and large brood sizes. The majority of C. elegans...