...NB, Brown M, Talbot D, Berg DE. pBR322-derived multicopy plasmids harboring large inserts are often dimers...self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. 1998 Sep;29(...PMID: 9767582. Summers DK, Beton CW, Withers HL. Multicopy plasmid instability: the dimer catastrophe hypothesis...

... The system is compatible with both monocot and dicot plants. Liu Lab Golden Gate/Gibson Assembly Multiplexing...and plasmids are available for both monocot and dicot plants, with a choice of either hygromycin or Basta...high-efficiency multiplex genome editing in monocot and dicot plants.” Molecular Plant 8(8) (2015): 1274-1284....

...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción...parte oficial de nuestra colección de libros electrónicos. Descargar CRISPR 101: Una herramienta de edición...

...teach students around the globe and is pushing SciComm further with his new company, Science with Tom.... scicomm...

...PubMed Preferences” and click on the “Filters & Icons” link (see image to the right). Once on the “Filters...” and search for “Addgene”. Then check the “Link Icon” box next to the “Addgene Nonprofit plasmid repository...

... bottom right corner of each page there’s a blue icon displaying the word “Help” next to an encircled ...wherever you are on our site. Simply click on the icon, search for answers, and choose a result. By clicking...

... of this system is the single-color labeling; multicolor labeling would enable the visualization of multiple...excited to see what’s coming next! References Multicolor CRISPR labeling of chromosomal loci in human ...

....1918.) Near-infrared fluorescent proteins for multicolor in vivo imaging. Shcherbakova and Verkhusha (...10;4:2153. doi: 10.1038/ncomms3153. PubMed) Multicontrast photoacoustic in vivo imaging using near-infrared...

...at eScience Labs. To learn how Caitlin got into scicomm and landed her first post-grad school job, head... scicomm...

...Internship with Addgene, I didn’t know a lot about scicomm, but had enjoyed writing a few Addgene guest blog...pieces. Throughout my internship, my interest in scicomm has grown and now it feels like an awesome bionerd...

...Rivera Solís, Universidad Autónoma de Yucatán, Mexico Dr. Rodrigo Arturo Rivera Solís, Associate Professor...technologies to protect fresh water repositories in Mexico. At Addgene we're happy to be able to support diverse...

...like any other youngster. His parents, Kristi and Rico, had to keep him from running too much as any jarring...which people dress up like superheroes or other iconic characters and run a 5K around Cambridge. We will...

...other fluorescent proteins to create an array of multicolor reporters that will enable imaging of multiple...Apoptosis: a review of programmed cell death." Toxicologic pathology 35.4 (2007): 495-516. PubMed PMID: ...

...you will have. When possible, use low-bind or siliconized tubes to store samples as it is more difficult...anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways for defense ...

...fluorescent proteins Commonly used filter sets for multicolor imaging include ones designed for CFP, YFP, and...compartments. I hope this sheds some light on multicolor imaging with fluorescent proteins. With the right...

...are receptors for endocrine steroids (i.e., corticosteroids, progesterone, androgens, and estrogens), fat-soluble...reporter gene has over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic ...

... for a Twitter account. Check out the hashtag #scicomm and start following science communication societies...and job offers. If you live in the UK, join the SciComm mailing list. 7) Internships – Learn on the job...

...a significant amount of QC. First, we perform Picogreen quantification on each and every isolated plasmid... the library preparation process and includes Picogreen quantification of various intermediate steps, ...

...the quantity of DNA after a PCR reaction as the amplicon doubles every reaction cycle. However, it is common...goal is to amplify a specific target of DNA. This amplicon can be used in molecular cloning to create a plasmid...

...T4 DNA ligase. Inserts, either precloned or in amplicon form (shown above) are flanked by BsaI sites in...efficiency measurements using similarly sized Lambda amplicon inserts to control for any suppression of background...