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Showing: 41 - 60 of 127 results

  1. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre–...

  2. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...lab at the university that I went to. Making that switch from being a grad student to an employee, and being...life changed––she had a daughter. Needing a career switch because of the demanding hours in the lab, she ...

  3. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...FRET sensors, can reduce these unwanted variables. Twitch is a FRET-based calcium reporter derived from troponin...sensor for neuroscientists is CaMPARI. This sensor switches from green fluorescence to a red fluorescent state...

  4. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...including many things people already use. It makes the switch to Golden Gate Cloning easier because individual...with Golden Gate MoClo. The barrier for those switching to Golden Gate is a conceptual barrier. Sometimes...

  5. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...Photoactivatable / Photoconvertible: These proteins can switch their color when activated by a specific excitation...the mEOS proteins. Some proteins are reversibly switchable (e.g. rsEGFP, Dreiklang). Fluorescent Timers ...

  6. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...transcriptors,” transistor-like digital genetic switches that enable cellular computing. These logic gates...fluorescence. There are a few examples of engineered RNA switches, but we've not yet seen the development of enough...

  7. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ..., compromising the photon budget. Likewise, the switch from ensemble imaging to single-molecule imaging...best method for live cell labeling and the easiest switch from fluorescent proteins given the relative simplicity...

  8. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...cultured rat hippocampal neurons. HaloTag dyes are switched from yellow to red at 0 h and back to red at 12...N.P., et al. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced...

  9. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...important ease of use is for them (if they are to switch from paper to digital for example): “… 99% of respondents...retrievable, searchable and traceable. However, switching from paper to an electronic lab notebook requires...

  11. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...importantly, you really are selling yourself. A sales pitch that requires reading two pages or even a paragraph...paragraph to get to the punch line is a terrible sales pitch. State in the first sentence or two what your research...

  12. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...from this kit include the production of a suicide switch in cyanobacteria, synthetic inverters in E. coli...biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis ...

  14. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...relatively cheap and easy to source, making it easy to switch from one readout method to another or change primary...’ll have to use different concentrations if you switch from one to the other.  Type of gel The type of...

  17. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...Quantitation by Kate Harten DeMaio We made the switch to droplet digital PCR to quantify AAV titers. ...

  18. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...or off, literally at the flip of a (blue) light switch. Zhang’s team devised the plasmids now available...

  20. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley, Shreya Vedantam, Michelle...
Showing: 41 - 60 of 127 results