We narrowed to 129 results for: itch
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TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....threshold like a switch that turns on or off. CRISPR shows a more continuous response - not switch-like at all...
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Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre–... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m....lab at the university that I went to. Making that switch from being a grad student to an employee, and being...life changed––she had a daughter. Needing a career switch because of the demanding hours in the lab, she ... -
Viral Vectors 101: Calcium Sensors
TypeBlog PostPublishedAug. 10, 2023, 1 p.m....FRET sensors, can reduce these unwanted variables. Twitch is a FRET-based calcium reporter derived from troponin...sensor for neuroscientists is CaMPARI. This sensor switches from green fluorescence to a red fluorescent state... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....including many things people already use. It makes the switch to Golden Gate Cloning easier because individual...with Golden Gate MoClo. The barrier for those switching to Golden Gate is a conceptual barrier. Sometimes... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....Photoactivatable / Photoconvertible: These proteins can switch their color when activated by a specific excitation...the mEOS proteins. Some proteins are reversibly switchable (e.g. rsEGFP, Dreiklang). Fluorescent Timers ... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....transcriptors,” transistor-like digital genetic switches that enable cellular computing. These logic gates...fluorescence. There are a few examples of engineered RNA switches, but we've not yet seen the development of enough... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m...., compromising the photon budget. Likewise, the switch from ensemble imaging to single-molecule imaging...best method for live cell labeling and the easiest switch from fluorescent proteins given the relative simplicity... -
Hot Plasmids - November 2023
TypeBlog PostPublishedNov. 14, 2023, 3:35 a.m....cultured rat hippocampal neurons. HaloTag dyes are switched from yellow to red at 0 h and back to red at 12...N.P., et al. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m....therefore acts as a switch between the default RFP and CFP, while Brainbow 2.1 switches from the default ... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog PostPublishedJuly 10, 2018, 1:08 p.m....important ease of use is for them (if they are to switch from paper to digital for example): “… 99% of respondents...retrievable, searchable and traceable. However, switching from paper to an electronic lab notebook requires... -
Tips for Getting a Faculty Position
TypeBlog PostPublishedJuly 20, 2023, 1:15 p.m....importantly, you really are selling yourself. A sales pitch that requires reading two pages or even a paragraph...paragraph to get to the punch line is a terrible sales pitch. State in the first sentence or two what your research... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog PostPublishedSept. 14, 2016, 2:30 p.m....from this kit include the production of a suicide switch in cyanobacteria, synthetic inverters in E. coli...biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis ... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog PostPublishedMarch 21, 2024, 1:15 p.m....photostability, and most importantly, a photoswitching ability. This photoswitching refers to the ability to ‘toggle... -
Technical Design of a Western Blot
TypeBlog PostPublishedAug. 22, 2024, 1:15 p.m....relatively cheap and easy to source, making it easy to switch from one readout method to another or change primary...’ll have to use different concentrations if you switch from one to the other. Type of gel The type of... -
MassBioEd Career Exploration Day at Addgene
TypeBlog PostPublishedNov. 13, 2015, 3:30 p.m....always learning and that means it is possible to switch your field.” Abigail was also impressed with the... -
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog PostPublishedJuly 26, 2018, 12:46 p.m....Resources on the Addgene Blog Learn about photoswitchable kinases Learn more about fluorescent biosensors... -
Addgene's Top Blog Posts from 2020
TypeBlog PostPublishedDec. 29, 2020, 2:15 p.m....Quantitation by Kate Harten DeMaio We made the switch to droplet digital PCR to quantify AAV titers. ... -
Let There Be LITE Plasmids
TypeBlog PostPublishedNov. 21, 2013, 3:56 p.m....or off, literally at the flip of a (blue) light switch. Zhang’s team devised the plasmids now available... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog PostPublishedJan. 17, 2018, 2:57 p.m....For instance, you might use it as part of a flex switch you’d like to work with in neurons or you might...