...AAV-PHP-B, it can be produced with high viral yields relative to AAV9. At lower viral titers than AAV-PHP.B ...when paired with the ubiquitous CAG promoter, is relatively biased toward L5 and inhibitory neurons and astrocytes...C57BL/6J and BALB/cJ mice through LY6C1, with relatively even distribution of target gene expression (...Spatial transcriptomics shows that its tropism is relatively more biased toward excitatory neurons than AAV-PHP.eB...Evolutionary tree depicting the phylogenetic relationship of common research species. Among these species...DNA levels (~25-fold and ~4-fold respectively) relative to AAV9 in the pigtail macaque brain slices. Similarly...

...genotyping, is critical to demonstrating causal relationships between genotype and assayed phenotype. Verifying...and T7E1 have been widely adopted due to their relative simplicity and low cost. The problem with these... detection rates mean the investigator can be relatively certain that their samples don’t have off-target...take into account other elements, including those relating to DNA structure (e.g. epigenetic modification...

.... Western blots are frequently used to compare relative levels of protein expression between cell types...protein can be used in experiments to compare the relative amount of protein present in a series of samples...interest from among the mixture and to measure the relative amount of your protein of interest on or in these...signals (up to a dozen or more!) to measure the relative or absolute amount of the expression of multiple...

...You know qPCR is most commonly used to measure relative quantification of mRNA, which can be used as a...reported to work well in the Lightcycler while being relatively easy to multiplex (Thellwell, 2000). See Table... Accuracy is not as important as precision for relative quantification. A consistent 5% positive error...Accurate and statistically verified quantification of relative mRNA abundances using SYBR Green I and real-time...

...of the method. One observation was an inverse relationship between deletion size and frequency [3]. There... DNA function is typically inferred based on correlative biochemical characteristics and ectopic reporter...   Like the video? The Orkin and Bauer lab's related paper describes their methods for CRISPR design...

...to detach from the constant stream of thoughts related to lab research and gain fresh perspectives. Such...encourages exploration of tangents or seemingly unrelated ideas. It's beneficial to carry a notebook at ... identify areas in need of improvement. These unrelated ideas might hold the key to unlocking new perspectives...

...access to coronavirus-related plasmids and to receive important coronavirus-related deposits. Our valiant...vector distribution is recovering from the pandemic-related slowdown. More scientists are sharing plasmids ...

... of ApoB-LPs has been shown to have a strong correlation with cardiovascular disease and indirect measurement...that the system is active in E. coli and closely related species, is amenable to use with other bEBP/UAS...and the system may not be adaptable to distantly related species from E. coli. This tool should serve as...

...the reaction on an agarose gel to determine the relative sizes of the resulting DNA fragments. Restriction...) shows 3 possible plasmid conformations, with relaxed and nicked marked with asterisks (*). When the ...conformations the most abundant being: supercoiled, relaxed and nicked. If your digest lanes look like your...

...Cancer and the Immune System: Deciphering the Relationship” by our guest blogger Subhadra Jayaraman, a ...platform focused on analysing immune measures and correlating them with tumor mutations and clinical responses...Cancer and the Immune System: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids...

...is software product management, and how does it relate to science? In the simplest of terms, a software...project management  Bolster your skills in strong relationship-building and influence. You’ll need a large ...large deal of influence and strong relationship-building in order to rally many stakeholders behind a common...

...our engineers (Fig. 2). Creating this guide was relatively easy. I took a picture of virtually every container...important documentation, including SOPs or project-related documents, we define new and potentially ambiguous...transferable skills Find all of our science career related blog posts Read our science communication blog ...

...widefield imaging fluorophores are excited with relatively low light levels (for a prolonged time). Other...experiments. As the photobleaching rate is not linearly related to power, the conclusions reached at high power...application (Goedhart et al, 2012). The third issue relates to the environment of the fluorescent protein. ...

...future prospects. Among the topics discussed, many related to the structure of the biomedical workforce. Quotes...results, not scientists”  These comments are well correlated with published data. For example, in 2010, less...grants through the Sloan foundation for postdoc-related data collection and analysis. He is also a strong...

...scientists are aware of the benefits of coaching relationships and do not know where to look for a coach. Many...thinking about what your goals are for the coaching relationship. Do you want to change jobs? Grow your professional...the coach to define how you will know that the relationship has been successful – what are the desired outcomes...

...Within the animal kingdom, this information is relayed through factors (both of endocrine and dietary ...systematic effort to compile NR- and coregulator-related global gene profiling datasets in to our data mining...coregulators. We look forward to growing this important relationship with Addgene for the benefit of the entire research...

...identical antibodies (See Fig 1.). rAbs are a relatively new technology that takes advantage of DNA sequencing... NOT align with the atlases/literature and is unrelated to the target protein, we categorize the antibody...strong signal. However, exposure time and its relationship to signal depend on what you’re comparing your...

...   Epigenetics nation stuck with methylation correlation  In the 1980s, a lot of things were cool: Walkmans...problem is that many of these studies report correlations without clearly testing whether a certain methylation... dCas9-TET, allowing one to study the causal relationship between DNA demethylation and gene expression...

...embark on or continue their careers in science-related fields. In particular, two recent events have the...note is a ruling in August by the National Labor Relations Board to allow graduate students who work as teaching...university and alterations to the student-university relationship, along with, of course, union dues. Time will...

... are enriched relative to controls using Split-BioID-GFP and BioID run on six unrelated proteins. One ...These interactions are often modeled as binary relationships, i.e. protein A interacts with protein B; but...