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We narrowed to 274 results for: sars

Showing: 41 - 60 of 274 results

  1. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...truthfully and in clear terms. It isn’t necessary to be cruel, but is necessary to be direct and clear. There is... too long, a small correction can become an unnecessarily big issue for you and for the employee. Make...

  2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...increase the utility of CRISPR/Cas9, it will be necessary to improve its multiplexing capacity. Multiplexing..., the modification of multiple genes is often necessary. Guide RNAs (gRNAs) are commonly packaged in 400...bacteria), and only a few elements of tRNAs are necessary for recognition and cleavage. This sequence flexibility...

  3. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...BioRender template. Since DnaA binding is necessary to initiate replication, controlling the amount...for the host, as no additional components are necessary. Iterons are not the only tool to stringently ...differ in the specialized proteins or sequences necessary for initiation or the mechanisms that regulate...

  4. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...training behind you (and largely only that is necessary), there are opportunities to work for biotechnology...their technical support, and a PhD is not always necessary. The job description and the candidate requirements...empathetic, and tactful. Occasionally it can also be necessary to take the lead and be assertive. You develop...

  5. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...which contains the regulatory regions that are necessary for myosin activity and thus interaction with ...actually a Drosophila meiotic arrest gene that is necessary for spermatogenesis. Flies that lack cookie monster...spatially restricted to the dorsal mesoderm, is necessary to activate bagpipe which is localized to segmental...

  6. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...what constitutes the “appropriate” codon is not necessarily obvious. You might think, “Nonsense! I should... above, not every region of a protein should necessarily be translated rapidly to produce a protein that...optimize a gene with one of these tools, it doesn’t necessarily mean the gene is going to express well. If you...

  7. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...eBook Here 1. Have a competitive CV You don't necessarily need a Nature/Cell/Science paper. You do need...can suffocate an exciting idea in a swamp of unnecessary details. You DO NOT need to present a detailed...an interview, your previous success will not necessarily translate into getting a job. Your papers and...

  8. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...plant-specific changes to CRISPR components are necessary to enable genome editing in plant cells.   This...modifications to commonly used CRISPR plasmids are necessary to use the CRISPR system in plant cells. Like ...Ti "helper" plasmid, which contains Vir genes necessary for T-DNA processing and insertion into the plant...

  9. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...abundance, and thus difficult to purify in quantities necessary for study. Moreover, the ability to isolate purified...strain for pSer-incorporation The second component necessary for phosphoserine incorporation is a modified ...interest as possible, some optimization will be necessary. To begin, one should optimize protein production...

  10. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...enough funds to get started, wasting funds on unnecessary materials, or even a loss of the offer all together...usually focus on the numbers, rather than the necessary resources. This is why at Experimental Designs...too often, we see early investigators making unnecessary short term sacrifices that hurt their goals, ...

  11. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...and validation Can’t forget the controls! The necessary control experiments will vary depending on your...standard IF. Here, we’ll walk you through the necessary types of controls for a multiplex experiment.  ...Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M...

  12. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...reproducibility. For a study to be “reproducible” does not necessarily mean that someone has replicated it precisely...them upon completing an experiment. There’s not necessarily in-principle acceptance, but readers of pre-registered...practices including talking to statisticians when necessary and encouraging interlab peer review of experimental...

  13. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...withholding necessary information Overwork – including undue pressure, impossible deadlines, unnecessary disruptions...undermining personal integrity, destructive innuendo and sarcasm, persistent teasing, name calling, insults, intimidation...

  14. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...), quantifying antibody/antigen interactions (Saraheimo et al., 2013), and visualizing structural changes...FRET acceptor labeling specificity is not always necessary for successful FRET experiments. If the acceptor...40.3 (2006): 279-286. PubMed PMID: 16908183. 10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach...

  15. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...permeabilization techniques — and so it may be necessary to try multiple techniques. For example, we have...When expression plasmids are available, or the necessary expertise to clone them, it can be useful to produce...to find for many proteins. In practice, it was necessary for us to combine overexpression with the use ...

  17. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...bad mentor. Apparently, a good Jedi does not necessarily an excellent mentor make. Are your current mentors...developing colleagues even as I reinforce the necessary power dynamic between advisor and students.” Stay...

  18. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ... special year for Addgene: it marks our 20th anniversary. Throughout the year, we’ll be celebrating with... You can enjoy these stories, and other 20th anniversary celebrations, by checking out our blog, social...

  19. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...published. This policy gives us time to complete necessary quality control and legal approvals so the plasmids...time later. We recognize that a preprint isn’t necessarily a clear sign that you are ready for us to release...

  20. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad control of whole circuits... circuits or tissue-wide systems is necessary, use chemogenetics. Note that, as with all tools, both approaches...
Showing: 41 - 60 of 274 results