...contributed by guest bloggers and IMP organizers Rosario F. Godino, Rocío López-Diego, & Zafira Castano ...and all over the world, she thought that it was necessary to develop a single platform to connect talented...students experienced great improvements, not necessarily quantifiable, but always significant. Doubts,... her on twitter @zcastan or on linkedln.       Rosario Fernandez-Godino is a Postdoctoral Researcher at...

...truthfully and in clear terms. It isn’t necessary to be cruel, but is necessary to be direct and clear. There is... too long, a small correction can become an unnecessarily big issue for you and for the employee. Make...

...increase the utility of CRISPR/Cas9, it will be necessary to improve its multiplexing capacity. Multiplexing..., the modification of multiple genes is often necessary. Guide RNAs (gRNAs) are commonly packaged in 400...bacteria), and only a few elements of tRNAs are necessary for recognition and cleavage. This sequence flexibility...

...which contains the regulatory regions that are necessary for myosin activity and thus interaction with ...actually a Drosophila meiotic arrest gene that is necessary for spermatogenesis. Flies that lack cookie monster...spatially restricted to the dorsal mesoderm, is necessary to activate bagpipe which is localized to segmental...

...BioRender template. Since DnaA binding is necessary to initiate replication, controlling the amount...for the host, as no additional components are necessary. Iterons are not the only tool to stringently ...differ in the specialized proteins or sequences necessary for initiation or the mechanisms that regulate...

...training behind you (and largely only that is necessary), there are opportunities to work for biotechnology...their technical support, and a PhD is not always necessary. The job description and the candidate requirements...empathetic, and tactful. Occasionally it can also be necessary to take the lead and be assertive. You develop...

...what constitutes the “appropriate” codon is not necessarily obvious. You might think, “Nonsense! I should... above, not every region of a protein should necessarily be translated rapidly to produce a protein that...optimize a gene with one of these tools, it doesn’t necessarily mean the gene is going to express well. If you...

...eBook Here 1. Have a competitive CV You don't necessarily need a Nature/Cell/Science paper. You do need...can suffocate an exciting idea in a swamp of unnecessary details. You DO NOT need to present a detailed...an interview, your previous success will not necessarily translate into getting a job. Your papers and...

...plant-specific changes to CRISPR components are necessary to enable genome editing in plant cells.   This...modifications to commonly used CRISPR plasmids are necessary to use the CRISPR system in plant cells. Like ...Ti "helper" plasmid, which contains Vir genes necessary for T-DNA processing and insertion into the plant...

...abundance, and thus difficult to purify in quantities necessary for study. Moreover, the ability to isolate purified...strain for pSer-incorporation The second component necessary for phosphoserine incorporation is a modified ...interest as possible, some optimization will be necessary. To begin, one should optimize protein production...

...enough funds to get started, wasting funds on unnecessary materials, or even a loss of the offer all together...usually focus on the numbers, rather than the necessary resources. This is why at Experimental Designs...too often, we see early investigators making unnecessary short term sacrifices that hurt their goals, ...

...reproducibility. For a study to be “reproducible” does not necessarily mean that someone has replicated it precisely...them upon completing an experiment. There’s not necessarily in-principle acceptance, but readers of pre-registered...practices including talking to statisticians when necessary and encouraging interlab peer review of experimental...

...and validation Can’t forget the controls! The necessary control experiments will vary depending on your...standard IF. Here, we’ll walk you through the necessary types of controls for a multiplex experiment.  ...Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M...

...withholding necessary information Overwork – including undue pressure, impossible deadlines, unnecessary disruptions...undermining personal integrity, destructive innuendo and sarcasm, persistent teasing, name calling, insults, intimidation...

...), quantifying antibody/antigen interactions (Saraheimo et al., 2013), and visualizing structural changes...FRET acceptor labeling specificity is not always necessary for successful FRET experiments. If the acceptor...40.3 (2006): 279-286. PubMed PMID: 16908183. 10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach...

...permeabilization techniques — and so it may be necessary to try multiple techniques. For example, we have...When expression plasmids are available, or the necessary expertise to clone them, it can be useful to produce...to find for many proteins. In practice, it was necessary for us to combine overexpression with the use ...

...Unearthed Comics” by Sara Zimmerman   5)   “Beatrice the Biologist” by Katie McKissick       Sara Zimmerman, Unearthed...

...bad mentor. Apparently, a good Jedi does not necessarily an excellent mentor make. Are your current mentors...developing colleagues even as I reinforce the necessary power dynamic between advisor and students.” Stay...

... special year for Addgene: it marks our 20th anniversary. Throughout the year, we’ll be celebrating with... You can enjoy these stories, and other 20th anniversary celebrations, by checking out our blog, social...

...spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad control of whole circuits... circuits or tissue-wide systems is necessary, use chemogenetics. Note that, as with all tools, both approaches...