We narrowed to 219 results for: ttl
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TypeBlog PostPublishedOct. 13, 2022, 1:45 p.m....your experiment, ensure your sgRNA sequence has little homology to other regions of the genome and consider... a very small peptide. In these cases, you have little flexibility as to where your sgRNA needs to be ...
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5 Reasons to Use Reddit for Science Communication
TypeBlog PostPublishedOct. 20, 2016, 2:30 p.m....people until the day of the AMA. This made it a little difficult to promote the AMA on social media beforehand...relationships. I also like Twitter because I can get a little opinionated and, while it can be anonymous, I can... -
Tips for CRISPR Gene Editing in Mice
TypeBlog PostPublishedJune 28, 2016, 10:59 a.m....should be able to find your mutant from screening as little as 8-10 pups. The level of fluorescence you saw...day you have your F0 generation (barring life’s little accidents and stuff ups). This is a huge improvement... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m....entities on our planet, but we still know collectively little about how they infect and influence the evolution...be choc-full of open reading frames that display little to no homology to anything else, making it difficult... -
Important Considerations When Using AAVs
TypeBlog PostPublishedJune 13, 2017, 2:30 p.m....Bitter but Its Fruit Is Sweet The Future? I knew little about adeno-associated Viral Vectors (AAVs) before...AAV-based tools to study neural control. Surprisingly, little research has compared and contrasted optogenetic... -
A Guide to Getting Started in Undergrad Research
TypeBlog PostPublishedJune 8, 2021, 1:15 p.m.... scientist. What is research? I grew up knowing little to no biological or biomedical scientists and had...towards grad students and postdocs—people who are a little ahead along their scientific career. I’ve found... -
Transferable Skills Guide: Teamwork
TypeBlog PostPublishedNov. 28, 2017, 2 p.m....have started that nickname myself). I have my own little Product Management Team, but I’m also an emeritus...intelligent, motivated person really could know a little bit about everything or in Jefferson’s case, even... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog PostPublishedOct. 8, 2020, 1:15 p.m....commonly used for every cell culture. Reusing the same bottle of serum again and again for each subculture can...Water baths, liquid nitrogen containers, reagent bottles and other sources of infection must be cleaned ... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m....about how protein networks work in cells. A major bottleneck, however, to these IP-based assays is to find...were transfected in 96-well format using an Amaxa Shuttle device; 5 days after the transfection, the entire... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog PostPublishedNov. 3, 2015, 3:30 p.m....strewn across the floor. In the final wake it all settles down and the value of each item changes from what...individual; the community as a whole has yet to settle on any true kind of value. I’ve dubbed this the... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog PostPublishedSept. 3, 2020, 12:15 p.m....photoactivatable CRISPR gene activation system, but settled on a different optimal fusion protein combination... the protein’s ability to cleave targeted DNA, settling on photocaging the K866 lysine, as seen in the... -
Delivery Methods for Generating iPSCs
TypeBlog PostPublishedApril 17, 2018, 1:37 p.m....cells with a large nucleus, large nucleoli, and little cytoplasm. Positive staining for alkaline phosphatase...scenario requires higher levels of safety and as little chance of genomic alteration as possible, with ... -
Introducing...Addgene Quick Tips!
TypeBlog PostPublishedSept. 19, 2024, 3 p.m....improve your lab techniques — but perhaps are a little limited on time — check out Addgene’s new Quick... -
Overwhelmed? Take a Break With Our 5 Favorite Science Comics
TypeBlog PostPublishedDec. 5, 2013, 3:27 p.m....our regular post-holiday routines, why not take a little time to appreciate the humor in science and in ... -
Congrats to the 2019 Deck The Lab Winners
TypeBlog PostPublishedJan. 17, 2020, 3 p.m.... at the University of Michigan decorated with a little bit of everything: frog snowflakes, ornaments, ... -
The Codon Guide to Synthetic Biology
TypeBlog PostPublishedSept. 14, 2023, 1:15 p.m....surprisingly, who have deep technical knowledge but little biology experience.) The Codon Guide to Synthetic... -
New Videos: Addgene Lab Tips
TypeBlog PostPublishedFeb. 15, 2018, 2:41 p.m....in the lab that will hopefully make your life a little easier. These can range from technical tips as ... -
Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew
TypeBlog PostPublishedFeb. 6, 2018, 2 p.m....difficulties of getting a lab up and running, and learn a little bit about what it was like for Wei Leong to work... -
Deck the Lab 2022!
TypeBlog PostPublishedJan. 17, 2023, 2:15 p.m....right now, so today noone could see my (not so) little friend anymore :C pic.twitter.com/rExV3b8Q8u— ... -
5 Science Rap Videos & Their Creator: Tom McFadden
TypeBlog PostPublishedFeb. 9, 2017, 3:30 p.m....Franklin vs. Watson & Crick - Science History Rap Battle A great example of science story telling following...