We narrowed to 749 results for: SOM

1. Cpf1 Update: Comparison to Cas9 and NgAgo

   Type

   Blog Post

   Published

   July 14, 2016, 2:30 p.m.

   ...discovered, researchers wondered if it could overcome some of Cas9’s shortcomings. Cpf1’s preferred PAM is ...

2. Plasmids 101: FLEx Vectors

   Type

   Blog Post

   Published

   April 28, 2016, 2:30 p.m.

   ...allowing for rescued expression of GeneR.   Sometimes also referred to as DiO (Double-floxed inverse...

3. Tips for Titering Your Lentiviral Preps

   Type

   Blog Post

   Published

   March 15, 2016, 2:30 p.m.

   ...comparing different batches of virus. In addition, some viral preps are very sensitive to freeze thaw cycles...

4. Design Tips for Prime Editing

   Type

   Blog Post

   Published

   Jan. 23, 2025, 2:15 p.m.

   ...many have tool-specific best practices. Here are some tips for optimizing your tool of choice. Nicking...

5. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ...trafficked and regulated. In this blog we will explore some of the most commonly used as well as newly developed...

6. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ...treatment should be apparent after three dsRNA feedings. Some of the phenotypes are not evident until after amputation...

7. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

   Type

   Blog Post

   Published

   July 23, 2020, 1:20 p.m.

   ...Cascade-Cas3, Cas9, Cas12, and Cas13 (Marino et al., 2020). Some Acr proteins are specific to a particular Cas protein...

8. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

   Type

   Blog Post

   Published

   May 7, 2020, 1:15 p.m.

   ...cells. While editing single genes is very useful, sometimes, you need to modify multiple genes to generate...

9. Mouse Modeling, Part 1: Genetically Engineered Mice

   Type

   Blog Post

   Published

   July 11, 2019, 1:26 p.m.

   ... over the injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-...

10. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    Published

    May 4, 2021, 1:15 p.m.

    ...often performed via phage display, yeast display, ribosome display, or mammalian display (Tsuruta et al.,...

11. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: New ...

12. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Expanded...

13. Plasmids 101: Modular Cloning

    Type

    Blog Post

    Published

    March 12, 2024, 1:15 p.m.

    ...which a precursor molecule is made, modified (sometimes many times), and isolated afterwards. To engineer...

14. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...looser or different requirements. Luckily, there are some available.   Cas proteins that don’t utilize NGG...

15. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...make mistakes. What if a tube is mislabeled or someone grabs the wrong plasmid? What if bacteria snuck...

16. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ...truncated gRNAs (≤15nt) have been found to retain some ability to induce Cas9 to make edits (AC, WLC, and...

17. Plasmids 101: Knockout/Knock-In Plasmids

    Type

    Blog Post

    Published

    Dec. 1, 2016, 3:30 p.m.

    ...of the targeted gene will be removed from the chromosome and and replaced by the resistance gene.The gene...

18. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...actually lower for xCas9 3.6/3.7 than for SpCas9. For some non-promiscuous gRNAs, xCas9 3.7 displayed more ...

19. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    Published

    Oct. 12, 2017, 2:26 p.m.

    ...discovery of many cellular mechanisms, there are some limitations to working with FPs. Overexpression ...

20. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    Published

    Feb. 7, 2019, 2:16 p.m.

    ...blog Read our CRISPR 101 blog posts Learn about some considerations when designing gRNAs Find other CRISPR...