We narrowed to 777 results for: REP

1. Cultivating Community Science at BosLab

   Type

   Blog Post

   Published

   Nov. 2, 2017, 1:35 p.m.

   ...diverse range of projects at BosLab. Some are entrepreneurs using our space to perform early-stage experiments...

2. Four Factors that Differentiate the Stem Cell Field

   Type

   Blog Post

   Published

   Sept. 28, 2016, 2:35 p.m.

   ...Cells Resources on the Addgene Wesbite Find Reprogramming Plasmids Find Other Hot Plasmid Tools Visit ...

3. How to Lead a Great Meeting

   Type

   Blog Post

   Published

   Feb. 9, 2016, 3:30 p.m.

   ...ambassador” from each affected team to be the representative in the meeting and then communicate outcomes...

4. Addgene Welcomes John Doench to the Advisory Board

   Type

   Blog Post

   Published

   Aug. 13, 2015, 2:30 p.m.

   ...function really…. Addgene’s role as a central repository for what is useful and what was used once and...

5. Management for Scientists: Delegating is Key

   Type

   Blog Post

   Published

   Feb. 20, 2015, 8:30 p.m.

   ...shared document. Update frequently with progress reports, meeting minutes and plan changes. Written documentation...

6. Choosing Your Fluorescent Proteins for Multi-Color Imaging

   Type

   Blog Post

   Published

   Oct. 9, 2014, 3 p.m.

   ...fluorescent proteins with this filter set and have reported brightness measurements. Here, EGFP outperforms...

7. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

   Type

   Blog Post

   Published

   Aug. 28, 2014, 3:34 p.m.

   ...lanes 4-5), there is a single band of 7.3 kb representing the full size of the plasmid. The double digest...

8. Finding and Joining Your Dream Lab

   Type

   Blog Post

   Published

   April 8, 2014, 2:50 p.m.

   ...the PI on any paper. Or perhaps, the lab has a reputation for not entering collaborations. Alternatively...

9. A Novice's Perspective on Neuroscience - SfN2017

   Type

   Blog Post

   Published

   Dec. 15, 2017, 2:07 p.m.

   ... efficiently. For select viral vectors in the repository, you can instead have Addgene make the virus ...

10. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type

    Blog Post

    Published

    Feb. 19, 2019, 1:36 p.m.

    ...important resource that Addgene provides is an open repository of plasmids and other necessary information that...

11. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    Published

    Jan. 8, 2019, 1:54 p.m.

    ...plasmid from the Zhang lab expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and...

12. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    Published

    Dec. 6, 2018, 1:34 p.m.

    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...

13. Improved Plasmid Maps Powered by SnapGene

    Type

    Blog Post

    Published

    June 29, 2017, 1:06 p.m.

    ...updates will make it much easier to browse the repository and quickly identify the plasmids and features...

14. Luminescent Imaging with Nano-lanterns

    Type

    Blog Post

    Published

    May 25, 2017, 2:30 p.m.

    ...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using...

15. Building and Validating Recombinant Antibodies: A Case Study

    Type

    Blog Post

    Published

    April 5, 2022, 1:20 p.m.

    ...becomes limited, which can be a threat to future reproducibility. The Trimmer Lab found that in addition to ...

16. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    Published

    Feb. 1, 2022, 2:15 p.m.

    ...not just the antibodies that matter when you’re prepping for your experiment - there are a number of outside...

17. Antibodies 101: Isotypes

    Type

    Blog Post

    Published

    Oct. 19, 2021, 1:15 p.m.

    ...valency of 10 antigens. An extra constant domain replaces the hinge region in IgM antibodies. IgM has no...

18. Viral Vectors 101: The AAV Retrograde Serotype

    Type

    Blog Post

    Published

    April 27, 2023, 1:15 p.m.

    ..., 2022) and substantia nigra (Tervo, 2016). Discrepancies in transduction efficiency can have a major ...

19. Antibodies 101: Flow Cytometry Controls

    Type

    Blog Post

    Published

    June 6, 2024, 1:50 p.m.

    ...interest. Using isotype controls An isotype sample is prepared very similarly to a FMO sample: you make a sample...

20. A Deep Dive into BioBuilder

    Type

    Blog Post

    Published

    Oct. 11, 2017, 1:44 p.m.

    ... The BioBuilder High School Apprenticeship, preparing local (Massachusetts), economically disadvantaged...