We narrowed to 762 results for: SOM

...numerous genomic loci through proteomics and the chromosome conformation capture (3C) technique. In addition...

...activation domain (CRISPRa) to regulate gene expression. Some CRISPR libraries contain many sgRNAs per gene to...

...promoter. Although safer, this system may be more cumbersome to use and lead to lower viral titers due to ...

... that CRISPR, the bacterial immune system, may someday be used to target the hardest-to-kill bacteria....

... a different nuclease could potentially overcome some of Cas9’s shortcomings - namely its blunt double...

...cells The Zhang lab made the exciting discovery that some Cas13a orthologs can function in mammalian and plant...

...  While finding smaller Cas9 orthologs works for some applications, it doesn’t eliminate the need to deliver...

...system SHERLOCKv2 for SHERLOCK version 2. Here are some of the improvements: SHERLOCKv2 uses far less primer...

...suggest that the late replication that occurs for some types low copy plasmids during cell division simply...

...poorly at body temperature (37oC) and were too burdensome to express in situ. As a result, they had to ...

...Every few months, we highlight some of the new plasmids, antibodies, and viral preps in the repository...

...complexity of effectively deploying them in the lab somewhat mirrors the complexity of understanding their ...

...mysteries of behavior. We’ve previously discussed some of the general considerations for performing behavioral...

...sequenced. Guide RNAs targeting several known and some unknown regulators of neuronal differentiation were...

...References:  Björck L, Kronvall G (1984) Purification and some properties of streptococcal protein G, a novel IgG-binding...

...high-throughput compatibility. However, there are some instances where we use long-read sequencing or Sanger...

...modifications Site-specific deletions and knockouts Some experiments may require the introduction of a mutation...

...research, the role of the MECP2 gene, and highlights some of the latest breakthrough therapeutic approaches...

...of writing many of the specifics surrounding why some gRNA’s work better than others remain unknown, but...

...easy technique, but it is very important to follow some simple rules to avoid common pitfalls and to use...