We narrowed to 749 results for: SOM

1. Finding nucleic acids with SHERLOCK and DETECTR

   Type

   Blog Post

   Published

   April 16, 2020, 1 p.m.

   ...system SHERLOCKv2 for SHERLOCK version 2. Here are some of the improvements: SHERLOCKv2 uses far less primer...

2. Plasmids 101: Plasmid Incompatibility

   Type

   Blog Post

   Published

   March 31, 2020, 1:15 p.m.

   ...suggest that the late replication that occurs for some types low copy plasmids during cell division simply...

3. New Tools Enable CRISPRa for Neuroscience Applications

   Type

   Blog Post

   Published

   July 2, 2019, 12:51 p.m.

   ...sequenced. Guide RNAs targeting several known and some unknown regulators of neuronal differentiation were...

4. Deciphering the Mysteries of Behavior with Viral Vectors

   Type

   Blog Post

   Published

   Sept. 17, 2019, 12:40 p.m.

   ...mysteries of behavior. We’ve previously discussed some of the general considerations for performing behavioral...

5. Rett Syndrome: A History of Research and Therapeutic Outlooks

   Type

   Blog Post

   Published

   Nov. 19, 2020, 2:15 p.m.

   ...research, the role of the MECP2 gene, and highlights some of the latest breakthrough therapeutic approaches...

6. CRISPR 101: Targeting RNA with Cas13a (C2c2)

   Type

   Blog Post

   Published

   Sept. 21, 2017, 2:07 p.m.

   ...cells The Zhang lab made the exciting discovery that some Cas13a orthologs can function in mammalian and plant...

7. Tips for CRISPR Gene Editing in Mice

   Type

   Blog Post

   Published

   June 28, 2016, 10:59 a.m.

   ...of writing many of the specifics surrounding why some gRNA’s work better than others remain unknown, but...

8. Tips for Using FRET in Your Experiments

   Type

   Blog Post

   Published

   Nov. 5, 2014, 3:41 p.m.

   ...easy technique, but it is very important to follow some simple rules to avoid common pitfalls and to use...

9. CRISPR 101: Homology Directed Repair

   Type

   Blog Post

   Published

   Jan. 26, 2023, 2:15 p.m.

   ...double-strand break repair and their potential to induce chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (...

10. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type

    Blog Post

    Published

    Feb. 22, 2017, 3:30 p.m.

    .... The “sloppy” PCR reaction is created by making some or all of the following modifications: 1) increased...

11. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type

    Blog Post

    Published

    May 5, 2020, 1:15 p.m.

    ... CDC uses RT-qPCR tests to diagnose COVID-19 and some serological tests to determine past exposure. However...

12. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type

    Blog Post

    Published

    Dec. 2, 2019, 2 p.m.

    ...protein molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013): 1050-...

13. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    Published

    April 9, 2019, 1:13 p.m.

    ...strategies to generate fusion proteins and highlight some aspects of their design.  Protein size and shape...

14. The Fluorescent Vegetables in Aptamer Soup

    Type

    Blog Post

    Published

    Jan. 29, 2019, 2:27 p.m.

    ...activates Spinach fluorescence. In the search for something more stable for quantitative imaging, Ming Hammond...

15. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    Published

    June 23, 2016, 2:30 p.m.

    ...pSer-incorporated protein of interest as possible, some optimization will be necessary. To begin, one should...

16. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    Published

    Jan. 26, 2016, 3:30 p.m.

    ...26721684. 4. Reardon S. Gene-editing summit supports some research in human embryos. Nature. 2015 Dec 3. doi...

17. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...tendency of fluorophores to aggregate in the neurons’ somata. Second, the system did not permit analysis by ...

18. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    Published

    June 24, 2020, 5:45 p.m.

    ...inducing double strand breaks. Below you'll find some of the CRISPR-based epigenetic modifiers available...

19. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type

    Blog Post

    Published

    April 5, 2018, 12:42 p.m.

    ...to purify Cas9 protein. The table below contains some of our favorites, all of which have blue flames....

20. Important Considerations When Using AAVs

    Type

    Blog Post

    Published

    June 13, 2017, 2:30 p.m.

    ...AAVs to use for your research. Below I’ll provide some tips that will help you think about how to get started...