We narrowed to 771 results for: SOM

...actually lower for xCas9 3.6/3.7 than for SpCas9. For some non-promiscuous gRNAs, xCas9 3.7 displayed more ...

...truncated gRNAs (≤15nt) have been found to retain some ability to induce Cas9 to make edits (AC, WLC, and...

...used to perform genome-wide screens and highlight some of the reagents that have been made publicly available...

...of Miami. The beginning of this century has seen some major advances in light microscopy, particularly...

...reaction is often unreliable, we suggest using the “Somewhat similar sequences (blastn)” program for blastn...

...promoter. Although safer, this system may be more cumbersome to use and lead to lower viral titers due to ...

... that CRISPR, the bacterial immune system, may someday be used to target the hardest-to-kill bacteria....

...inducer virus. Additionally, this system is less cumbersome than brainbow, another method for multi-color...

...activation domain (CRISPRa) to regulate gene expression. Some CRISPR libraries contain many sgRNAs per gene to...

... be incredibly valuable to scientists, there are some potential complications that must be considered ...

...careful selection of FP fragments and, preferably, some information about the structures of the tested PIPs...

...numerous genomic loci through proteomics and the chromosome conformation capture (3C) technique. In addition...

... a different nuclease could potentially overcome some of Cas9’s shortcomings - namely its blunt double...

...of writing many of the specifics surrounding why some gRNA’s work better than others remain unknown, but...

...modifications Site-specific deletions and knockouts Some experiments may require the introduction of a mutation...

...References:  Björck L, Kronvall G (1984) Purification and some properties of streptococcal protein G, a novel IgG-binding...

...cells The Zhang lab made the exciting discovery that some Cas13a orthologs can function in mammalian and plant...

...high-throughput compatibility. However, there are some instances where we use long-read sequencing or Sanger...

...Every few months, we highlight some of the new plasmids, antibodies, and viral preps in the repository...

...  While finding smaller Cas9 orthologs works for some applications, it doesn’t eliminate the need to deliver...