We narrowed to 855 results for: NTS

...They provide accounts of their research struggles in papers. They recount accomplishments in talks and... pay attention. Highlight your lowest points and highest points and make clear what was difficult and ...you don’t like it yourself. Be proud of your achievements and accept and acknowledge your failures. Foster...

...monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color...therefore can be used in immunoprecipitation experiments with other fluorescent proteins. Find mNeonGreen... coli with a gene library of approximately 484 variants, including some potential tetramer-breaking mutations...a protein which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and...

...academia, it is especially important for graduate students and postdocs to get a perspective on other types...use of resources and pay attention to budget constraints Academic science is much like industry but the...science’s sake More opportunity to train and teach students Collaborations may be less “complicated” - for...tell you to pick up the pace Constant worry about grants and renewals What is different about non-academia...apple can change entire culture) Specific requirements for hiring more common Likelier to hire on ...

...professional societies hosting virtual networking events. Download Addgene’s Science Career guide to learn...reproducibility best practices Support your future experiments and other researchers by implementing reproducibility...out Reproducibility for Everyone. Plan out new experiments carefully. Consider submitting a registered report...consider using this framework to design your future experiments.  Use Addgene’s AAV Data Hub. You can search ... the AAV Data Hub for tools to use in future experiments. If you’ve used Addgene’s AAV tools, you can ...

...while sipping an afternoon coffee in between experiments. The longer we are in this flexible work schedule...people use is the built-in whiteboard, in which participants can contribute and sketch on the board without...time breaks up the monologue a little and gives participants something else to focus on. Use collaborative... creativity are pivotal in writing publications/grants too. As described by several works (Wuchty et al...be done randomly or manually assigning which participants to group together. Breakout Rooms can be used...

...culture and in live zebrafish. For cell culture experiments, only dL5**-expressing cells treated with MG-...neighboring bystander wild-type cells.   Zebrafish experiments with MG-2I-dL5** were performed with embryos ...Zebrafish are an ideal model organism for these experiments because developing embryos are transparent, so...also show that MG-2I-dL5** only generates small amounts of non-specific ROS when exposed to YFP and RFP...your experiences using photosensitizers in the comments below!   References 1. He, J., Wang, Y., Missinato...

...digested with ribonucleases, ribosomes protect the segments of mRNA to which they are bound. These regions...color switching, TRICK requires the following components: a GFP protein that binds to the coding region...the mRNA to the plasma membrane; this sequence prevents diffusion of the mCherry labeled mRNA and keeps...second. They also explored the effects of 5’ UTR variants in translation of cell cycle protein Emi1, showing...coding sequence and 3’ UTR. Before beginning experiments, it’s important to verify that these changes ...

...Zhou and Fatima Enam. There were many excellent applicants but Xin and Fatima stood out for their creativity...learn more about Xin’s work with photoswitchable components of the ERK signaling pathway in our recent blog...first design is a whole cell biosensor with two components - an enzyme that breaks down the HMO of interest...communication skills throughout her training and wants to make her work understandable to people both inside...Choose the Brightest Fluorescent Protein for Your Experiments Resources on Addgene.org Find Fluorescent Protein...

... production of viruses used to deliver CRISPR components to cells or organisms under study. In the past...a single cell line and proceed to use it for experiments.” - Alex Chavez, researcher at the Wyss Institute...particularly prominent problem in CRISPR genome editing experiments. We aim to alleviate this frustration by producing...primary goal. Getting these quality-controlled reagents from Addgene can greatly expedite a researcher's...our viral services. Please send any questions or comments to [email protected]. Finally we’d like to thank...

... inactive dCas9 molecules. We followed these experiments with more detailed investigations into the release...delivery by nucleofection The in vivo editing experiments in this publication were performed using a technique...well as the sequences of our validated editing reagents can be found on our plasmid pages. We encourage...encourage any labs performing genome editing experiments to try nucleofection as (in our hands) this technique...transfection approaches. Moreover, the cost of reagents is relatively modest, requiring expressed Cas9...