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We narrowed to 842 results for: NTS

Showing: 381 - 390 of 842 results

  1. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ... this system to develop a bacterial camera that prints a chemical image, as well as a genetic method for...experimented with the promoter strength of various components, removing inducible promoters that could crosstalk...increased dynamic range to around 100-fold. These improvements will allow researchers to better fine-tune the... input light intensity. In addition to these improvements, the constructs have been greatly streamlined...larger systems. The work of Schmidl et al. also represents a large step forward in optimizing TCSs. Over...

  2. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...does one stand out amongst a pool of successful applicants and get chosen to work in his or her lab of choice...documented history in science. Potential graduate students typically go through a rotation, which reveals...we do fits in with what they want to do. The experiments must fit with their interests.” George Church.... Alternatively, perhaps the lab publishes and presents regularly with labs around the world. You may ...the happy dance at least once in awhile. Acknowledgments Thank you to Dr. Tom Ellis (Imperial College...

  3. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...throughput? Will it be sensitive enough? No one wants to spend time screening hits that aren’t “real” ...line possessing a patient mutation to assess how patients may respond to drug treatment. Another example...particular disease as the input population. For these experiments, the goal is to identify manipulations which ...manipulations are assessed, rather than all manipulation events. To put it another way, only cells or organisms...condition that can apply enough pressure to select for variants of interest, though. A selection can take the ...

  4. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...implement these improvements. Let us know what you think about the updates in the comments section below... in action). Read on to learn more about the improvements we’ve implemented and how they’ll make it easier...on. Beyond these simple but powerful display improvements, when you click on any plasmid map, you also...checking in at blog.addgene.org for additional enhancements that will further improve the map displays and...

  5. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...race to find vaccines, therapeutics, and other treatments to stem the spread of COVID-19. That’s why we...you used plasmids from this collection in your experiments and published your data, please fill out this...expression vectors containing various spike protein variants are also available. Plasmids are continually added...proteins, and membrane proteins. Although all viral components are available in bacterial expression plasmids...purification of SARS-CoV-2 proteins for downstream experiments and offer flexibility in tag, cleavage, codon...

  6. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...and editing speed and consists of the following components: A plasmid in the host bacterium expressing ...infectious. The editing speed dictates which phage variants can propagate faster than the rate of dilution...uninfected host cell culture). This dilution step represents the major difference between PACE and PANCE: ...stringency PACE, resulted in the evolution of TadA variants with much higher base editing activity compared... TadA-7.10 enzyme. After testing numerous TadA variants, they identified TadA-8e, which edits 590-fold...

  7. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    Published
    Nov. 19, 2019, 2:08 p.m.
    ...controlling cell signaling. Key components of OptoNBs OptoNBs have two key components: an AsLOV2 domain and a ...activity in cells.  Let’s shed some light on the key components of OptoNBs, how they are generated, and their...variable heavy chain antibody domain.  How do these components interact? When fused together to form an OptoNB...below table summarizes the results for these experiments.  AsLOV2 Inertion Site mCherry #1 mCherry ...

  8. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...facets of life. It also seems as if many of the experiments in neurobiology (and really in all of biology...minimal immunological effects. AAVs are used in experiments targeting genes to specific cell types in the...us how useful the service has been for their experiments. This helped reinforce our goals of making more...the Addgene blog to get some ideas for future experiments and to find tools that will make your transition...many biological fields the number potential of experiments enabled by physical, software, and biology-based...

  9. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...involving protein stability or binding experiments. Potential experiments that one might perform using the ...transcripts for degradation. Despite how easy these experiments are to physically set up in the lab, RNAi is ...the specific gene producing long unspecific RNA fragments that are incorporated into the dsRNA necessary...membranes, organelles, and other subcellular compartments is via proteomics--the study of the distribution...

  10. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...make sure that they’ll be compatible with your instruments, each other, and your target. I love FPbase’s...reaction, can be detected using specialized imaging instruments or X-ray film and lends itself well to assays...consider a broader selection of antibodies for your experiments.  Many of the methods for antibody conjugation...If needed, you can often remove the incompatible agents by dialysis or gel filtration.    There is, of ...Conjugates: From Heterogeneous Populations to Defined Reagents. Antibodies 4:197–224. https://doi.org/10.3390...
Showing: 381 - 390 of 842 results