...reproducing useful natural materials.      Moore et al., ACS Synthetic Biology 202. https://doi.org/10.1021/acssynbio...

...biosensors and displayed her findings at the Spring 2017 ACS meeting. She hopes these biosensors and her future...that these enhanced “pdDronpa” domains could be placed in amino acid loops on either side of an enzyme...using this sensor to detect HMOs found on cellular surfaces. After incubating the sensor with bacteria engineered...

...sensors using self-associating fluorescent domains. ACS Chem Biol. 2013 Oct 18;8(10):2133-9. doi: 10.1021...field. Those engineered sensors were designed to replace synthetic calcium dyes, which had been in use since...particular advantage for observing events taking place inside cells that are on the move, such as T lymphocytes...FRET-based sensor proteins, particularly for the intracellular imaging of transition metal ions, such as zinc...

... you could obtain arrays A, B, C, AB, AC, BC, and ABC). Nielsen Lab E. coli CRMAGE Plasmids: The CRMAGE...Multiplex Transcriptional Repression in E. coli.” ACS Synthetic Biology 4(9) (2015):987-1000. PubMed PMID...nontargeting spacer flanked by two CRISPR repeats. The spacer can be digested using BsaI, allowing a spacer-repeat...using 3 distinct spacer-repeats, you could easily create 7 unique arrays (e.g. for spacers A, B, and C, you...promoter-gRNA construct, but with different sequences adjacent to the sites. When digested with the appropriate...plasmids are digested, unique overhangs (here, O1-4) adjacent to the cut sites “link” fragments together and...

...two-component systems. Schmidl SR, Sheth RU, Wu A, Tabor JJ. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021...

...PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. ...

...in more contexts. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR...developed the Phage-Assisted Continuous Evolution (PACE) and Phage-Assisted Non-Continuous Evolution (PANCE...mutations, selection, and replications per day.  The PACE and PANCE systems use M13 bacteriophage, which rely...dilution step represents the major difference between PACE and PANCE: for PANCE the phage progeny is not diluted...-stringency PANCE, followed by higher stringency PACE, resulted in the evolution of TadA variants with...

...AsLOV2 protein binder plasmids here! Reis JM, et al. ACS Synthetic Biology. 2018 PubMed PMID: 30203962. ...

... modular assembly of broad-host-range plasmids." ACS synthetic biology 7.5 (2018): 1279-1290. PubMed PMID...

...Streptomyces Species Using an Engineered CRISPR/Cas System. ACS Synth Biol 4:723–728 . https://doi.org/10.1021/sb500351f...for Modular Assembly of Broad-Host-Range Plasmids. ACS Synth Biol 7:1279–1290 . https://doi.org/10.1021/... most CRISPR-based genome engineering has taken place in other organisms. In many bacteria, unlike other...and chloramphenicol resistance pCRISPR: carries a spacer targeting the gene of interest and kanamycin resistance...template, and the locus cannot be recognized by the spacer-derived crRNA. However, if recombineering is unsuccessful...survive have inactivating mutations in the Cas9 or spacer sequence, and these rare events are easily detectable...successfully in Tatumella citrea, another species of Enterobacteriaceae, without the need for modification. This ...