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  1. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
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    Feb. 27, 2020, 2:15 p.m.
    ...through your organization in this field. Please also enter the billing address for your organization. We will...customer service line or sent an email to help@addgene.org, you’ve likely talked to one of us! Today we’...you time. Anyone affiliated with the recipient organization can create an Addgene account and place an order... entered during checkout. At this time, your organization will have net 30 days to complete payment for...
  2. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
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    Sept. 2, 2014, 3:05 p.m.
    ...conference here. The Future of Research conference is organized by postdocs for postdocs. I am delighted to see...importance of the topics to be discussed, the organizers are building valuable skills and meeting new ...Students also partner with independent career organizations to get the grants and programs they need such...offices in the last few years. However, some organizations are woefully behind and some just don't have...Symposium on the Future of Science: A postdoc-organized symposium on the future of the scientific endeavor...
  3. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
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    March 17, 2021, 2:40 p.m.
    ... on the advisory board for Art in Giving, an organization that raises money for childhood cancer through...art. About two years ago, I had a big role in organizing an Art in Giving think tank event where we brought...proposal, which Art in Giving was able to fund. Organizing that event and seeing the outcome was wonderful...wonderful!  I also volunteer for a dog rescue organization where I foster dogs and help screen dog adoption... Subscribe to the Addgene blog Resources on addgene.org: Meet the other members of Addgene's Board of...
  4. Fluorescent Proteins 101: History of Fluorescent Proteins

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    Blog Post
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    Aug. 7, 2017, 1:58 p.m.
    ...These molecules occur naturally in bioluminescent organisms, but their real power lies in the clever ways ...Although humans have known about bioluminescent organisms and their characteristic radiance for some time...to isolate two proteins from the jellyfish’s photoorgans; the first, which he called aequorin, gives off...collaborator Martin Chalfie expressed GFP in exogenous organisms (E. coli, and later C. elegans) for the first ...be responsible and began studying these marine organisms to find the culprits. He even obtained samples...
  5. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
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    June 17, 2015, 9:04 p.m.
    ...as a free event! Organizing a science conference for the public How does one organize a public scientific...association with the Harvard graduate student organization Science in the News (SITN), we recently sponsored...communication skills and engage with the public. The organization runs a wide variety of programs, including fall...opportunities to improve our communication and organizational skills provide great training for a number ...
  6. Why Do I Need an MTA Anyway?

    Type
    Blog Post
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    May 1, 2014, 8:23 p.m.
    ... for more details. Benefits to the provider (organization / lab that owns the material) Affirmation ...materials will only be shared with non-profit organizations Assurance that recipient may not use the ...Limited liability Benefits to the Recipient (organization / lab receiving the material) Permission ...directly to your institution’s MTA coordinator. Organizations that have opted into our eMTA system can even...knowledgeable staff is here to help. Contact us at mta@addgene.org or +1 617-225-9000, or check out our MTA reference...
  7. Finding Your Science Policy Path

    Type
    Blog Post
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    Sept. 22, 2020, 1:15 p.m.
    ...the bench, I have worked for seven different organizations in a variety of science policy roles including...Big P policy from a post in non-governmental organizations, universities, professional societies, and industry...is non-governmental work happening at large organizations who have to develop policies for their communities...on a neglected disease area. Depending on the organization, these changes to policy could shift how society...your time in graduate school through outreach organizations and science blogs. Other avenues for skill development...development include leadership of extracurricular organizations, working in student government, and teaching...
  8. Antibodies 101: Validation

    Type
    Blog Post
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    March 24, 2022, 1:15 p.m.
    ...Introduction to Antibodies blog post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene...on the antibodies. Nature 521:274–276. https://doi.org/10.1038/521274a  Bradbury A, Plückthun A (2015)...antibodies used in research. Nature 518:27–9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L...antibodies for research. Mabs 8:27–36. https://doi.org/10.1080/19420862.2015.1100787  Taussig MJ, Fonseca... the mountains. New Biotechnol 45:1–8. https://doi.org/10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski ...of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995  ...
  9. Are Hybrid Guide RNAs Right for Your CRISPR Application?

    Type
    Blog Post
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    Jan. 27, 2026, 2:14 p.m.
    ...the other hand, the tail sequence is much more forgiving and can be mostly or entirely replaced with DNA...Nucleic Acids Research, 48(15), 8601–8616. https://doi.org/10.1093/nar/gkaa605  Whittaker, M. N., Testa, L.... Nature Biomedical Engineering, 1–13. https://doi.org/10.1038/s41551-025-01545-y  Yin, H., Song, C.-Q...Nature Chemical Biology, 14(3), 311–316. https://doi.org/10.1038/nchembio.2559 Additional resources on the...Adenine Base Editors Additional resources on addgene.org Read the CRISPR guide Download the CRISPR 101...
  10. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
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    June 10, 2025, 1:15 p.m.
    ...transposases. Nature Protocols, 19(3), 752–790. https://doi.org/10.1038/s41596-023-00927-3 Klompe, S. E., Vo, P...integration. Nature, 571(7764), 219–225. https://doi.org/10.1038/s41586-019-1323-z Peters, J. E., Makarova...States of America, 114(35), E7358–E7366. https://doi.org/10.1073/pnas.1709035114 Witte, I. P., Lampe, G....transposase. Science, 388(6748), eadt5199. https://doi.org/10.1126/science.adt5199 Additional Resources on...Flexibility to CRISPR Editing Additional resources on Addgene.org Addgene's CRISPR Guide (Jump to Large Edits)...
  11. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
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    May 2, 2023, 1 p.m.
    ... screens to introduce reporters throughout an organism’s genome. These reporters have historically been...The best trap depends on your experiment and the organism you are working in. The spacing of genes and whether...proteome? Open Biol. (2019) 9:180241. DOI: https://doi.org/10.1080/rsob.180241 Vanni, C., et al., Unifying...sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps.....1105/tpc.12.7.1007  Additional resources on Addgene.org Vector database Intro to the Lab Bench Plasmids...
  12. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
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    June 19, 2018, 1:38 p.m.
    ...Fluorescent reporters are not as well suited for imaging organs located deep in the body, like the gastrointestinal...the reporter and fail to pinpoint what tissue or organ this signal is coming from.   Acoustic reporter...where the bacteria localize in relation to internal organs. The limit of detection for arg1 expressing ECN...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed...fluorescent protein reporter gene Resources on Addgene.org Find plasmids for in vivo imaging with fluorescent...
  13. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
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    Nov. 17, 2015, 3:30 p.m.
    ...Cambridge and within the general public. The society organises talks by prominent scientists, including Nobel...topics in their area of research. The society also organises PhD information events and summer internship information...support each other and provide advice. The society organizes numerous recreational and educational events throughout...for their students. Another large goal of the organisation is to encourage iGEM alumni to mentor younger...Bloggers Learn How to Get Involved in a Profession Organization ...
  14. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
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    March 28, 2022, 4:30 p.m.
    ...and impact of your work. Please email deposit@addgene.org if you would like to contribute to the catalog...share your plasmids via Addgene! Email deposit@addgene.org to learn more or get started.  If you have suggestions...immunolabeling of brain. eLife 8:e43322. https://doi.org/10.7554/elife.43322 Bandrowski, AE, Martone, ME...Experimental Methods. Neuron 90(3):434-6. https://doi.org/10.1016/j.neuron.2016.04.030    Bradbury A, Plückthun...antibodies used in research. Nature 518:27–29. https://doi.org/10.1038/518027a  Resources We would also like to...
  15. Viral Vectors 101: Transductions

    Type
    Blog Post
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    April 30, 2024, 1:15 p.m.
    ...depends on two things. First, what type of cell or organism context you are targeting? Second, what is the...moderate levels. With regard to target cells or organism, some primary cells and differentiated cell types...shine is when a specific cell type within a live organism is the target. Although rarely done, viral pseudotype...for a type or subtype of cells. This allows an organism to be injected with a virus, but enables tissue-specific... well. Transduce on! Resources Resources on Addgene.org Generating Stable Cell Lines with Lentivirus...
  16. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
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    Sept. 14, 2021, 1:15 p.m.
    ...Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org/10.1007/s13205-012-0070-8   Chakiath CS, Esposito...reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org/10.2144/000112585   Ding T, Huang C, Liang Z, Ma...Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186/s12934-020-01321-4   Kolodner R (1980) ...National Academy of Sciences 77:4847–4851. https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger J, Plank...efficiency or half-life. RNA 25:507–518. https://doi.org/10.1261/rna.069286.118 Additional resources on ...
  17. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
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    June 2, 2020, 1:15 p.m.
    ...precise titrations of AAV or heat-shocking the organism. The SPARC system uses competitive recombination...system that can be used in many cell types and organisms including Drosophila, fish, and mice, by replacing...labeling Isaacman-Beck et al., bioRxiv 2019. https://doi.org/10.1101/788679 A destabilized GFP reporter for... Diessl et al., Microbial Cell, 2020. https://dx.doi.org/10.15698%2Fmic2020.04.713 Truncated TALE-fluorescent... tTALE-FP Shin et al., Sci. Rep. 2019. https://doi.org/10.1038/s41598-019-53722-0 ...
  18. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
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    April 25, 2017, 2:30 p.m.
    ...are better for whole cell imaging and studying organelle trafficking. Monomeric PA-FPs are usually preferred...involve enhancing optical live imaging of proteins, organelles, and cells. Similar to FPs, PA-FPs allow for ...localization, turnover, and trafficking, as well as organelle trafficking and dynamics (fission, fusion, etc...extraordinary molecular detail.   References 1.Patterson, George H., and Jennifer Lippincott-Schwartz. "A photoactivatable...mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is blocked during...Catch Up on Your GFP Background Resources at Addgene.org Find Photoactivatable FPs in Empty Backbones...
  19. Cultivating Community Science at BosLab

    Type
    Blog Post
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    Nov. 2, 2017, 1:35 p.m.
    ...official by registering BosLab inc. as a nonprofit organization in the state of Massachusetts. Because we’re...local companies and BosLab has grown into an organization consisting of over twenty members and volunteers... involved, check out the local groups page on DIYBio.org to find a community lab in a city near you. To...Angela Kaczmarczyk! Angela Kaczmarczyk is a co-organizer at BosLab, a community biotech laboratory in Somerville...Plasmid Sharing Speeds Science Resources at Addgene.org Order Plasmids from Addgene! Find Free and Open...
  20. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...that selection of a fluorescent probe (be it an organic dye or FP) is one of the most important steps in...visualization of FP attributes (which turned into www.fpvis.org).  That page has been very useful over the years... and dissemination of information about FPs. FPbase.org FPbase is a project written in Python & Django...(so feel free to moderate your own version of fpvis.org with the proteins of your choosing!), and they...Proteins for Multicolor Imaging Resources on Addgene.org Browse all fluorescent protein plasmids Find...
  21. Advice for Scientists Starting a Lab

    Type
    Blog Post
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    April 29, 2014, 1:43 p.m.
    ...support. “It is an exciting and emotional time!” George Church tries to recruit people who are nice as ...“Treating people fairly does not mean equally.” George Church was surprised by “how much better it is ... to lead his team as a scientists and a friend. George Church runs his lab fairly similarly to his advisor... Or share your success stories below. And don't forget to stay tuned for the last post in this series ...Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking the ...
  22. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post
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    Feb. 11, 2021, 2:15 p.m.
    ...GPCRome. Nat Struct Mol Biol 22:362–369 . https://doi.org/10.1038/nsmb.3014 Paek J, Kalocsay M, Staus DP,...Proximity Labeling. Cell 169:338-349.e11 . https://doi.org/10.1016/j.cell.2017.03.028 Roth BL (2016) DREADDs...for Neuroscientists. Neuron 89:683–694 . https://doi.org/10.1016/j.neuron.2016.01.040 Takeda S, Kadowaki...genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016/s0014-5793(02)02775-8 Additional resources...our quick guide to chemogenetics Resources on Addgene.org Check out Addgene's chemogenetics guide Find...
  23. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
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    Oct. 20, 2022, 1:15 p.m.
    ...2020). https://pubs.acs.org/doi/10.1021/acscentsci.9b01093 More resources on addgene.org  View Addgene's...cells. Nat Biotechnol 33, 985–989 (2015). https://doi.org/10.1038/nbt.3290 Rozners, E. Chemical Modifications...Frontiers in Genome Editing, 28 (2021). https://doi.org/10.3389/fgeed.2020.617910 Zou, R., Liu, Y., Wu,...Molecular Cell 81, 7, 1553-1565 (2021). https://doi.org/10.1016/j.molcel.2021.02.007 Moroz-Omori, E., Satyapertiwi...
  24. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
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    Aug. 19, 2025, 1:15 p.m.
    ...https://www.addgene.org/browse/article/28248224/","href_with_scheme":"https://www.addgene.org/browse/article...scaffold of OCaMP) are shaded in orange. Data from FPbase.org. Scientists have developed calcium indicators...activity. bioRxiv 2025.07.28.667269; doi: https://doi.org/10.1101/2025.07.28.667269. Additional Resources...Fluorescent Protein Additional Resources on addgene.org Fluorescent Proteins: Biosensor Plasmids In-...
  25. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
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    June 29, 2021, 1:15 p.m.
    ...functional variable regions. mAbs 10:539–546. https://doi.org/10.1080/19420862.2018.1445456  Holzlöhner P, Hanack...Antibodies by Hybridoma Technology. JoVE. https://doi.org/10.3791/54832  Lipman NS, Jackson LR, Trudel LJ...Information Resources. ILAR Journal 46:258–268. https://doi.org/10.1093/ilar.46.3.258  Mould AP, Askari JA, Byron... Biological Chemistry 291:20993–21007. https://doi.org/10.1074/jbc.m116.736942  National Research Council...monoclonal antibodies for immunotherapy Resource on Addgene.org Check out Addgene’s antibody plasmid collection...
  26. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
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    May 26, 2020, 1:15 p.m.
    ... your CRISPR experiment was successful in your organism or cell type? You can use DNA sequencing or other...in Cell and Developmental Biology 6: . https://doi.org/10.3389/fcell.2018.00054 Sander JD, Joung JK ...genomes. Nature Biotechnology 32:347–355 . https://doi.org/10.1038/nbt.2842 Wen Y, Liao G, Pritchard T, ...of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074/jbc.m117.777722 Additional resources ...with our CRISPR 101 blog posts Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...
  27. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
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    April 13, 2017, 2:30 p.m.
    ...monitoring and analyzing autophagy of cytosol and organelles in yeast cells. Autophagy (Greek for “self-eating...by a lack of nutrients and targets cytosol and organelles to the vacuole/lysosome for degradation and recycling...more fine-grained detail about what proteins or organelles are being degraded) and it is slow. Alkaline...for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy,4(2), 205-213. doi:10.4161...Fluorescence Microscopy Additional Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent...
  28. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
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    Oct. 21, 2025, 1:15 p.m.
    ... acid bases already present throughout living organisms, Click Biology reactions can add new functionality...Addgene in multiple formats: for use in different organisms, targeted to particular compartments, or fused... Chemical Reviews, 121(12), 6697–6698. https://doi.org/10.1021/acs.chemrev.1c00469  Howarth, M. R. (2025...biological reactions. Nature Chemical Biology. https://doi.org/10.1038/s41589-025-01944-x   Additional Resources...Plasmids 101: Degron Tags Additional Resources on addgene.org New England Biolabs Cell-Imaging Plasmid Collection...
  29. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post
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    May 11, 2021, 1:15 p.m.
    ...National Academy of Sciences 104:5163–5168 . https://doi.org/10.1073/pnas.0700293104 Field J, Nikawa J, Broek...addition method. Mol Cell Biol 8:2159–2165 . https://doi.org/10.1128/mcb.8.5.2159 Galvan A, Raper J, Hu X, Paré...monkeys. Eur J Neurosci 50:2801–2813 . https://doi.org/10.1111/ejn.14429 Kimple ME, Brill AL, Pasker RL...Current Protocols in Protein Science 73: . https://doi.org/10.1002/0471140864.ps0909s73 Additional references...the many types of protein tags References on Addgene.org Check out Addgene's Chemogenetics Guide Find...
  30. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
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    March 8, 2017, 3:15 p.m.
    ...tech transfer team will communicate with your organization’s technology transfer office or other authorized...than 75 plasmids, please contact us at deposit@addgene.org. These deposits are considered large deposits... your PI and send the spreadsheet to deposit@addgene.org with the following information: Addgene account.... The name of the Principal Investigator and Organization where your plasmids were first created. From... You'd Like to Find at Addgene Resources on Addgene.org Start Your Deposit Deposit FAQ Which Plasmids...
  31. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
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    May 22, 2018, 1:38 p.m.
    ... a multi-gene pathway to study and a reference organism where deletion strains are available. Then, staff...clone similar sequences from a less-well studied organism into overexpression plasmids. Students next transform...transform deletion strains from the reference organisms with the overexpression plasmids. Finally, students...Saccharomyces. cerevisiae, makes a great reference organism for the project because it is non-pathogenic, ...Students Explore Careers at Addgene Resources on Addgene.org Find Yeast Expression Vectors Check Out Our ...
  32. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
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    Oct. 12, 2017, 2:26 p.m.
    ...been an invaluable resource for studying the organization and function of cellular systems. FPs have been...engineering FP fusions associated with cellular organelles, scientists have been able to study many cellular... fluorescent protein tagging with SapTrap The Jorgensen Lab recently developed a modular plasmid assembly... References Schwartz, Matthew L. and Eric M. Jorgensen. “SapTrap, a Toolkit for High-Throughput CRISPR...Use Rosella to Monitor Autophagy Resources on Addgene.org Check out the SapTrap Kit Page Find Other Fluorescent...
  33. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post
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    Sept. 12, 2017, 1:44 p.m.
    ...discuss. We are also available by phone or at help@addgene.org to answer your questions about our quality control...Delivery. Molecular Therapy 12:744–753 . https://doi.org/10.1016/j.ymthe.2005.06.003 Guerin K, Rego M, Bourges...Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277 Lecomte E, Tournaire B, Cogné...Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Wright JF, Zelenaia O (2011...NGS for Plasmid Quality Control Resources on Addgene.org Find Ready-to-Use AAV Find Ready-to-Use Lentivirus...
  34. Plasmids 101: Degron Tags

    Type
    Blog Post
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    March 14, 2024, 1:15 p.m.
    ...E3350-7. doi: https://doi.org/10.1073/pnas.1216880109  Resources on Addgene.org CRISPR Plasmids – Protein...studies. Nat Commun. 13, 5495 (2022). doi: https://doi.org/10.1038/s41467-022-33246-4  Chung HK, Jacobs CL...Chem Biol. 2015 Sep;11(9):713-20. doi: https://doi.org/10.1038/nchembio.1869  Nabet B, Roberts JM, Buckley...Chem Biol. 2018 May;14(5):431-441. doi: https://doi.org/10.1038/s41589-018-0021-8  Holland AJ, Fachinetti...
  35. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
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    July 12, 2018, 1:09 p.m.
    ...culture that will hopefully help you keep a well organized lab and contamination free environment for successful...should keep in mind that UV light only kills microorganisms that it strikes directly. UV light exposure...consistent growth rate. For every minute spent organizing, an hour is earned (Benjamin Franklin)! It’s ..."Ostreopsis cf. ovata induces cytoskeletal disorganization, apoptosis, and gene expression disregulation...Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your research Find...
  36. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...20(2), 111–114. https://doi.org/10.1093/mutage/gei016I Resources on Addgene.org Addgene’s Basic Molecular...Resistance Stability Time Saving?  Works for multiple organisms? Compatible with most bacteria? No satellite colonies...Clinical Microbiology, 23(5), 959–961. https://doi.org/10.1128/jcm.23.5.959-961.1986 Lobstein, J., Emrich...cytoplasm. Microbial Cell Factories, 11, 56. https://doi.org/10.1186/1475-2859-11-56 Trastoy, M. O., Defais,...
  37. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...our plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular biology and cloning...Using Glass Syringe Filters. PloS one. https://doi.org/10.1371/journal.pone.0007750  Vogelstein, B., &...United States of America, 76 2, 615-9 . https://doi.org/10.1073/pnas.76.2.615  Zhou, Y., Zhang, Y., He,...Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou, Y., Mason, M.G...microbes in under 30 seconds. PLoS biology. https://doi.org/10.1371/journal.pbio.2003916...
  38. 27 Hot Plasmids from 2016

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    Blog Post
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    Dec. 22, 2016, 3:03 p.m.
    ...construction of a so-called “genomically recoded organism” (3) but its portability to other bacterial strains...monitoring and analysing autophagy of cytosol and organelles in yeast cells. Named after the brightly-coloured... both in vitro cell systems and diverse model organisms. Addgene’s newest TALEN kit, the FusX assembly...has revolutionized genome editing in multiple organisms thanks to its simple, easily modifiable RNA-guided...screens required to identify correctly modified organisms has limited routine use of this technology. To...tag insertion in the C. elegans genome, Erik Jorgensen’s lab from the University of Utah developed SapTrap...conditionally knock down proteins in a variety of model organisms. As long as it was expressed in a cell that also...
  39. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
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    June 20, 2024, 1:15 p.m.
    ...Aspects of DNA Replication. IntechOpen. https://doi.org/10.5772/19313 References Asano, K., & Mizobuchi.... The EMBO Journal, 17(17), 5201–5213. https://doi.org/10.1093/emboj/17.17.5201 del Solar, G., Giraldo...Biology Reviews: MMBR, 62(2), 434–464. https://doi.org/10.1128/MMBR.62.2.434-464.1998 Grimwade, J. E.,...Nucleic Acids Research, 46(12), 6140–6151. https://doi.org/10.1093/nar/gky457 Park, K., Han, E., Paulsson,.... The EMBO Journal, 20(24), 7323–7332. https://doi.org/10.1093/emboj/20.24.7323 Resources at Addgene ...
  40. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...on the escape mechanism of genome editing in microorganisms which could be applied to optimization of genome... Appl Environ Microbiol 81:2506–2514 . https://doi.org/10.1128/aem.04023-14 Li Q, Sun B, Chen J, Zhang... Acta Biochimica et Biophysica Sinica. https://doi.org/10.1093/abbs/gmab036 Zhang Y, Sun X, Wang Q, Xu...Transposases. ACS Synth Biol 9:1998–2008. https://doi.org/10.1021/acssynbio.0c00073   Additional resources...all articles about microbiology Resources on Addgene.org: Visit the CRISPR Guide Get CRISPR plasmids ...
  41. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...one either. Choose the topic and outline the organization of the review Once you start reading, there ...references) and it’s better to keep your references organized from the beginning. I also recommend using the...form of figures/schematics or tables. And don’t forget to include citations so that people can go back...Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving Your Next Manuscript 6 Tips...Tips for Grant Writing Resources on Addgene.org Check out Our Hot Plasmids Articles to See Compiled Info...
  42. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...grow liver stem cells and liver-like mini-organs or organoids, neither of these can substitute for a liver...talk preparation: Define your research vision. Organize your vision into a chalk talk format. Practice...questions from open ended screens and data collection. Organize your research vision into the chalk talk format...Read all of our career blog posts Resources on Addgene.org Learn more about careers at Addgene Watch our...
  43. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...(CNS) orchestrates complex processes enabling organisms to control their movements and behavior. These...to study connectomics (how neural circuits are organized and how neurons connect to each other). Combined.... 2. Shepherd, G.M. (2004). In "The synaptic organization of the brain". Oxford University Press, New ...pdb-top63. PubMed PMID: 20150100. 4. Shaner, Nathan C., George H. Patterson, and Michael W. Davidson. "Advances...gene delivery to the CNS and PNS Resources on Addgene.org Find the Spaghetti Monster probes at Addgene...
  44. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...Awareness Organization (ADAO) are working toward such a ban, and the president of the organization, Linda... has spread from where it originated to another organ in the body. Such misdiagnosis and late discovery...tumors from the peritoneum and any other abdominal organs to which it may have spread. Then, the surgeon ... Screening in Ghana Additional Resources on Addgene.org Check Out Our Cancer Ressearch Collection Find...
  45. Students Invented a New Diagnostic for Lyme Disease — and a Tool for CRISPR Researchers

    Type
    Blog Post
    Published
    May 12, 2026, 1:15 p.m.
    ...biology. One 2025 team from Lambert High School in Georgia caught our attention when they cited our primer... Nature Biotechnology, 38(7), 870–874. https://doi.org/10.1038/s41587-020-0513-4  Chen, J. S., Ma, E.,...activity. Science, 360(6387), 436–439. https://doi.org/10.1126/science.aar6245  Guérin, M., Vandevenne...SELEX. Communications Biology, 8(1), 632. https://doi.org/10.1038/s42003-025-08034-7  Additional resources...101: Cas9 vs. The Other Cas(s) Resources on Addgene.org How to Design a Primer CRISPR Guide Addgene ...
  46. Cutting Once Is Harder Than It Sounds: Your Guide to Minimizing Off-Target Effects

    Type
    Blog Post
    Published
    March 31, 2026, 1:15 p.m.
    ...off-targets. Nature Communications, 14(1), 5746. https://doi.org/10.1038/s41467-023-41393-5 Kulcsár, P. I., Tálas...sgRNAs. Nature Communications, 11(1), 1223. https://doi.org/10.1038/s41467-020-15021-5 Tálas, A., Huszár, K...sgRNAs. Nucleic Acids Research, 49(6), e31. https://doi.org/10.1093/nar/gkaa1220  Wang, D., Zhang, C., Wang...learning. Nature Communications, 10, 4284. https://doi.org/10.1038/s41467-019-12281-8  Additional resources...Genome-Wide CRISPR Screen Design Resources on addgene.org The CRISPRecise Kit page includes detailed ...
  47. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...our days more effective, and our schedules more organized. From the alarm in the morning, to the daily news...of paper in notebooks, and hoping that we don’t forget to write things down when doing scientific research...note-taking tools, others using excel sheets or organizing folders in complex ways, there are solutions ...institution, the transition is smooth as all data is organized within an ELN already. Investing some time in ...makes it much easier for lab members to manage and organize their work at every step of the research process...
  48. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. ...amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite...Niemi, Ph.D., is a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. ... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156... Tags for Protein Purification Resources on Addgene.org Find Synthetic Biology Plasmids Find Bacterial...
  49. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...Alberta in Edmonton, Canada, to the University of Georgia, USA) has developed that allows for a phage genome...membrane on an agar plate selective for your target organism and incubate overnight according to appropriate...on hand that will specifically bind your target organism, it can be helpful to spot this protein directly...lab during its recent move to the University of Georgia). She enjoys pondering how phages get through the...CRISPR For Genome-Wide Screens Resources on Addgene.org Visit Our Microbiology Pages Use the FX Cloning...
  50. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...couldn’t be more excited! As a small, tight-knit organization we thrive on 3 things: fun, teamwork, and competition...will combine all of these. Addgenies have been organizing Halloween festivities for years – searching our...sweet – as sweet as all that Halloween candy you’ll gorge yourself on in the coming days. Hopefully, I’ve ...procrastinating choosing their costume?  As our organization has grown, we continue to have an amazing turnout...
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