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We narrowed to 749 results for: SOM

Showing: 61 - 80 of 749 results

  1. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...will give you some advantage over other candidates. Write about your transferable skills Some of the skills...'t think a resume is "one size fits all" like sometimes CVs are. Because you only have two pages, you ...collaboration between research groups? Perhaps you had some supervisory experience working with undergraduate...students? On top of that, perhaps you were involved in some extracurricular activities – did you volunteer to... about science? Or perhaps you have presented in some public lectures? These transferable skills could...think only undergraduates use these services, but some of the general knowledge about preparing a good ... dealing with interviews, is actually universal. Some universities even have officers working specifically...

  2. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...restriction digests on plasmids at Addgene, we sometimes observe something odd in our uncut DNA control: a band ...MiSeq sequencing that Addgene uses.) However, when some researchers received the sequencing results for ...form of the plasmid with repeating sequences. In some specific cases, multimerization has been observed...please note that the supercoiled dimer band can sometimes overlap with the band corresponding to the nicked..., it cannot be used to detect plasmid multimers. Some companies provide NGS whole plasmid sequencing services... reduce the frequency of multimerization, and in some cases completely prevent the formation of plasmid...Screen multiple colonies to isolate the monomer Sometimes a plasmid can exist in a bacterial population ...

  3. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...full-time position. For my part, I really enjoyed some aspects of the work, such as physically carrying...failure and problem solving. I believe that is something lacking during degree courses; they never really...the nursing students and community health workers some basic biology and first aid. This really allowed...the company was to fulfill contracts, my work sometimes felt rushed and the focus always seemed more on...skills in industry positions. There were, however, some downsides to working in a large commercial company...situation, communication between departments was sometimes difficult and the solution attempted was more ...solved with good management practices, but can sometimes go unnoticed at large organizations. Another downside...

  4. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...mental effort, and that some concepts are easier to grasp than others. But sometimes we’re unaware that we... a visual aid instead of describing something verbally. Sometimes a group was just really tired and needed...as it does to read an 11-page Cell paper - and sometimes, it might actually take me less time to read the...contributes to cognitive load?  Everything! Here are some common areas that can use up your audience’s mental...encouraged) to leave blank spaces or to gloss over some aspects when needed. The trick is to communicate...way I thought about engaging with learners. If something I was communicating didn’t click, instead of blaming...put their phones away), or maybe I was assuming something was intuitive that actually wasn't. Maybe I needed...

  5. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...viruses that can infect people, cells, and animals. Some of these viruses are completely inert, but others...controls, safety systems that are built into how something functions, were designed for this purpose. One...understand what safety precautions to take, and if something does happen, ensure you can receive appropriate...monitoring. Virus-specific concerns   In nature, some viruses are more dangerous than others, and the ...infection process (though it can integrate at chromosome 19q13.4 qtr. (AAVS1)). For these reasons, AAV...does not integrate into the host’s genome. However, some adenovirus strains are known to cause respiratory...peracetic acid, 10% bleach, or 1% iodine are useful. Some organizations or cities may require a higher percentage...

  6. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    Published
    Sept. 2, 2014, 3:05 p.m.
    ...Professor Emeritus at UCSF. Dr. Bourne has some radical (and some not so radical) suggestions for defining...worked with some great postdoc and grad student career offices in the last few years. However, some organizations...scientists can have successful careers doing science in some form? There is a lot of talk about the state of ...rumors that we are training too many scientists and some propose radical changes to the way we view the graduate...organizations are woefully behind and some just don't have the resources to do enough. There are still university...in taking matters into their own hands, there are some easy ways to get started. I am happy to work with...

  7. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...review the inspiration behind ExM, how it works, and some of the limitations to keep in mind whether you are...option, but the equipment needed for EM is not something you find in the average cell and molecular biology...digest the sample to allow for even expansion.  In some cases, labels are added after this stage.  Step...may be better for your specific targets. What are some of the limitations?  A “super-resolution” technique...sounds great, right? But there is always a catch - some limitation of the technique that users should be... continuous lines in pre-expanded samples can sometimes take on a fragmented appearance post-expansion...can be alleviated by maximizing label density. In some cases, nanobodies have been found to help improve...

  8. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...two different pairs of chromosomes using gRNAs specific to sequences on chromosomes 9 and 13. They next ...example, the visualization of chromosomal translocations or chromosomal shattering (chromothripsis). As...could then be used to examine both inter- and intrachromosomal dynamics, and how they change in response ...turned their attention to mapping pairs of intrachromosomal loci. The technique successfully resolved ... this work represents the first mapping of intrachromosomal loci, a major benchmark in characterizing ...next! References Multicolor CRISPR labeling of chromosomal loci in human cells. Ma H, Naseri A, Reyes-Gutierrez...

  9. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...helpful. I set out to make something practical and accessible. If there's something you come across that is...failure to clearly articulate one's talents and ideas. Some key points from my ebook, "Applying for a Faculty...your specialty area, it's a safe bet that at least some committee members will NOT be in your area. Therefore...solicited for feedback), possibly the Dean, and even some people in your field that still need to understand...The second question is whether there is chalk. Sometimes. It can also be a slide deck, markers, etc. Chalk... What is the big question? This should be something that anyone could relate to, that people would...students encouraged me to publish this manual and earn some cash for my expensive habits of running shoes and...

  10. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...integrate into the host cell’s genome. However, sometimes inte-great-ion isn’t so great. In this post, we...RNA genomes into DNA to be integrated into the chromosome of the cell. This integrated viral genome is ...generate long-lasting changes in cells. However, for some applications, integration might be considered a ... poised for integration, two types of circular episomes can be generated through either homologous recombination...Schematic representation of integration process and episomal forms of non-integrating transgenes. Created with...adapted from Banasik and McCray, 2010. These episomes are not maintained in dividing cells because they...). Over time, the expression diminishes as the episomes are lost through cycles of cell division, though...

  11. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...So you have this awesome experiment you want to do, but it requires some AAV. You’ve never worked with...as it’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins...like all good experiments, making AAV starts with some plasmids. You just need three plasmids to start ...their secondary structure and high GC content, but some companies now offer Sanger sequencing conditions...Other cis-regulatory elements Next let’s talk about some of the elements that aren’t required parts of an...video!   Now you’ve got a basic understanding of some common AAV plasmid elements! And while there are...

  12. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...I had to start somewhere. I knew if I started with the Harvard Business Review or some Chartered Institute... learn something about marketing would be to start reading a broad simple overview, learn some jargon ...research experience, a PhD or a Master’s degree and some marketing experience, with little emphasis placed... years and purchased their products, so you have some of the best ideas on how to sell these products ...of the basics of sales and marketing and reading some of these articles would have stressed me out more... ELISA kit company on whose website you can find some great information on ELISA troubleshooting, ELISA...

  13. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ...Another difficulty is that some articles are just harder to write. “There’s something about being a writer ...’s science communication journey started with something unexpected: termites. “I was really interested... work for one client and then switch to doing something else. You’re never really integrated and part ...pulled in all of these different directions is sometimes at odds with the space needed to be creative. ...a lot of time to let you mind process things. Sometimes finding that uninterrupted time to be creative...connection with a reader, whether it’s a scientist or someone who’s affected by climate change or affected by...these problems through connections and that’s something that good storytellers in science can do.” Susan...

  14. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...that, let’s take a look at exosomes. Proficient in transportation, exosomes are tools of communication...evasiveness, evolution, and response to treatment. Some microenvironment cells improve tumor’s durability...after injecting breast cancer cells into mice, exosomes expressed specific integrins (cell-adhesion proteins...proteins) targeting different organs. This means that exosomes not only promote microenvironment’s malignancy...also incite different organs to become malignant. Exosomes also contain miRNA (micro RNA) and other molecules...epigenetic changes in targeted cells. In this case, exosomes may transport miRNA from drug-resistant cells ...PMID: 22439926. Hoshino, Ayuko, et al. "Tumour exosome integrins determine organotropic metastasis." Nature...

  15. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...is sifting through the long list of titles for something that is actually useful to you. When embarking... the jargon and background in your field, but sometimes papers are also just poorly written. By nature... are showing you their data. They should explain some of the rationale behind the line of experiments,...they collected and what it means. There should be some connection or flow among the figures and results...ultimately builds up to an overall conclusion(s). In some articles, the data are presented to build a case...conclusion. Personally, I like papers that can end with some sort of model (cartoons are appreciated) to visually... literature in your field of interest. Set aside some time each day or week to at least scan the titles...

  16. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...constructive feedback from experts. If someone crosses out something, find out why. Ask successful writers...how can you do it well? Below, I briefly describe some tips for a successful postdoc fellowship. 1. Reverse...researcher. Depending on the position, you may also want some evidence of teaching experience. How do you get...experiment in your new lab, stop and plan it out. Some questions to answer include: What is the goal of...is preferable to finding out you've been doing something all wrong or that a reagent has not been convincingly...position was because the search committee asked someone at my institute about me, even though I had not...exercise. I highly recommend running or biking or something that demands your attention. Eat well- a diet...

  17. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...scientific endeavors. I hope that this post can provide some perspective and advice to any undergraduate interested...Professor, Assistant Professor, Associate Professor Sometimes teaches graduate and/or undergraduate classes ...thesis project to earn a degree "Rotation student" - some PhD programs have students rotate through a few ... I think reflecting on why it is we enjoy, or in some cases, don’t enjoy, is worthwhile and can help us...your way. Think critically about whether it’s something you want to dedicate your time and energy to. ...cultivate stronger relationships with my labmates. Sometimes what I thought was a dumb and very basic question...career advice and advancement, my labmates have been some of my closest friends and have often provided me...

  18. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...that can replicate independently from its host’s chromosome. Although we usually think of bacteria when we... cellular reproduction, similar to the host’s chromosome, or they can spread between hosts through horizontal...Penicillium isn’t the only organism that knows it. Some bacteria possess plasmid-borne toxins like colicins...environments — like by living where no one else wants to. Some organisms manage this with plasmids that allow them...factors can also be integrated into the bacterial chromosome, but when encoded on plasmids, they can promote... plasmids that don’t have known functions. While some of these plasmids likely have undiscovered functions...plasmids tend to be small and poorly studied, but some researchers have suggested they may have relevance...

  19. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...could have wanted to know was just buried beneath someone's lunch. Many times, I’m pleasantly surprised to... searches scientific articles, patents, and even some doctoral dissertations. Streamline the question...send us an e-mail. That’s why we’re here! Below are some pointers to expedite and improve your customer service...answer several emails that start: “Hi! We ordered something from you and it’s wrong. Can you help us fix it...weeks to resolve adequately. Occasionally there are some really off-the-wall questions (perhaps that’s a ...where on this range your question falls. Here are some things to keep in mind after you hit “send” or dial...the full answer to a complicated question may take some time. If the customer service rep does not tell ...

  20. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...speak about. There should be some spontaneity, but having a good handle on some interesting questions and... by listing some and asking people to raise hands in response. I am always ready with some written poll...and talking as a result of the content. Here are some of my tips to organizing and running a memorable...Joanne's Reddit AMA Perhaps we should start with some examples of a bad panel discussion? You know, the...when to move on to the next topic. There must be some spontaneity so a moderator should be prepared with...participation can be pre-planned if the moderator knows some of the people in the audience. I always try to meet...
Showing: 61 - 80 of 749 results