...careers of its researchers are on the line. With these priorities in mind, many researchers go to great ...Nature report from researchers at Amgen, it was stated that only 11% of the academic research in the literature...down when doing scientific research? Electronic lab notebooks and researcher's needs The familiar paper-...hundreds of researchers. The full story is available here. Our questions focused on researchers’ current ... designed for a specific research area, due to anxieties that their research ‘doesn’t fit neatly into ...enables researchers to store everything in one place. From pictures to excel sheets, researchers today ... records, but given the complexities of modern research, it’s often not enough. A 2013 study showed that...

...Novartis researcher, Carrie Bhang. The ClonTracer Library, deposited by Carrie Bhang, a research investigator...was actually really research oriented [and] somewhat similar to an academic research environment” with ...individual cell receives only one barcode. The abundance of each barcoded clone can then be monitored over...over time by sequencing the barcodes in the population (all barcodes can be amplified using the same sets...Looking at barcode abundances among multiple replicate populations, they found that similar barcodes consistently...Oncology, is an exciting new tool that allows researchers to individually label millions of mammalian cells...because she wanted first-hand experience in industry research. When she came to Novartis, she was happily surprised...

...collaborative platform to help researchers working on COVID-19. Its origins trace back to March 2020, when the first...areas of scientific research. We continue to support those involved in COVID-19 research, but our new platform...and María Hernández Sánchez from Crowdfight. In March 2020, while most of the world was locked down, a... while the early results were promising, the researchers did not have access to a P3 laboratory (BSL-3...a few weeks, we were able to connect them to a research group in Scotland, who had just the right infrastructure...which despite the competitiveness of cutting-edge research, is still one of the most valuable features of...science in our view. And it was this very idea that research is done better with others than by oneself that...

...fastest BLAST search to perform and will save you time compared to other types of BLAST searches. If you ...: blastx searches are inherently slower than blastn or blastp, due to the additional searches involved...drastically reduce waiting time for search results. Optimizing blastp searches Depending on the sequencing result...Addgene, we continually use the Basic Local Alignment Search Tool (BLAST) provided by NCBI. BLAST helps us compare... programs, databases, and elements of the BLAST search pages. At Addgene, we use blastn to identify any...into these differences below. Optimizing blastn searches On the Standard Nucleotide BLAST page, the first...sequences and may be the most comprehensive for searching. Timesaving Tip #1: If you know the species that...

..., 2013). Research using E. coli has the potential to address many areas of microbial research like metabolism...the most used mammalian model in medical research with an archive of information and data that has yet to...2016). Research proceedings on amphibian model organisms. Dong wu xue yan jiu = Zoological research, 37(...model organism, an essential aspect of biological research that has defined our understanding of biological...perfect candidate for introductory teaching and research environments (Idalia & Bernardo, 2017).  ...common use in plasmid manipulation and genetics research. Plasmids are ideal tools for studying genetic...engineering.  The E. coli genome has an extensive research history and characterization. Many proteins and...

...share my experiences from my own job search and my time on faculty search committees. Having successfully ...that their search committee is different and needs to be told all of the nuances of the research in as technical..., can appreciate their research. Be prepared to discuss other people's research. Nothing turns off an ...expert in [your research area]," – you should show interest in other faculty members’ research. Still a Grad...interviews sections, along with new thoughts on research statements. Snapp, who is always looking for ways...understand documents A common misconception is that search committee members are experts in your field. Not.... State in the first sentence or two what your research is about and what you think you can accomplish...

...laboratory research management consultant and coach in Boston, MA. He specializes in assisting research scientists...budget. Creating a budget We start by using your research specific aims. This is actually why a department...time it will take to secure your first (R-level) research grant.  Set your timeline from there, then start...talent pool that you’ll need to tap for your future research team. For example, if you’re going to need a tech...resources that you’ve thoroughly identified. Do online searches, and seek advice from your current administrative...information sites (like, indeed.com, or salary.com). Search industry and academic job titles in the regions...to identify which items aren’t critical to your research success. If you can get away with using computer...

...scientists writing research articles. What should you take into account when writing up your research? What type...subgroup of researchers in your area of expertise? Sometimes, the funders of your research may stipulate...more valuable to the research community. It also makes it more likely that researchers have enough information...Maaike Pols, PhD, a Developmental Editor at F1000Research.  With many changes taking place in the world...type of journal should I choose? Writing a research paper for publication in a scientific journal requires...increasing number of funding bodies insist that the research data resulting from their funding should also ...your Addgene plasmid #), and other reagents. F1000Research, among many other journals, has joined a pilot...

...typically writes the grants that fund your research, guides the research directions of the lab, gives you constructive...manuscript or fellowship reviewers, expose your research to search committees, and can lead to mutually beneficial...Student and Postdoctoral Programs at theJanelia Research Campus of the Howard Hughes Medical Institute....that attest to your talent and expertise as a researcher. Depending on the position, you may also want...during your postdoc At the end of grad school, you searched for and eventually identified a postdoctoral mentor...constructive feedback on your research, and teaches you how to write as well as mentor. Yet, even the most... least one more person that knows you and your research well. Who could fill these different roles? A ...

...provide them on your smart phone. 1. Lab Archives by LabArchives The single, most important part of lab ...instructional videos. The search function in the manual just highlights what the user searches, which is not very...heavily on adapting my smartphone to help me with my research in terms of preparedness, organization, and sharing... life. These apps prevent you from constantly searching the interwebs for lab resources, and instead conveniently...lab life is the lab notebook. Lab Archives is a multipurpose application that allows you to share your data...notebook from your smartphone to your computer. Lab Archives will help both you and your lab stay organized...from 2 years ago. The free subscription to Lab Archives allots for 25 MB of space per user, but, if you...

...methodologies and rigorous research plans. However, amidst the organized chaos of lab research and PhD studies, ... Widening your search The first step towards inviting serendipity into your research lies in expanding...structured world of lab research and PhD studies, where every aspect of research is meticulously planned... the potential to redefine the course of one's research journey — serendipity. The best scientific breakthroughs...enhancing your project's potential. Widening your search creates fertile ground for serendipitous encounters...become very focused on the details of a specific research question. However, breakthroughs often happen ...the constant stream of thoughts related to lab research and gain fresh perspectives. Such activities foster...

...parts of the world in my early research career. I've found that research institutes in different places...I believe that enChIP will markedly accelerate research of regulation mechanisms of genome functions in... enChIP, and I'm very happy to see that many researchers all over the world have requested plasmids for...cells. We believe that this system will allow researchers to use enChIP in a wide variety of biological...promote Addgene, which is also beneficial to my research. I believe that involvement in the scientific ...scientific community is a kind of duty for researchers when considering the peer-review system of journals and ...Addgene: Throughout your career, you have held research appointments in the US (New York University School...

...one visual barcode per cell and up to 200-plex with two barcodes per cell. The researchers validated their...visual barcodes for clonal multiplexing of cells in live imaging application. Each visual barcode consists...visual barcodes in use in A375 cell line. Image from Kaufman, et al, 2022.    This new barcoding technology... error rate for long sequence knock-ins Visual barcodes for live cell clonal multiplexing Improving ...interaction). The modularity of the system provides researchers better flexibility and makes the approach more...generate “single-use” transgenic lines. Instead, researchers can generate and maintain transgenic lines that...error rate. For a one kilobase insertion, the researchers reported an editing error rate of less than 0.3%...

...scientific community speed research is to offer plasmids created as part of research projects, another way ...Consortium collection enables research on solute carriers (SLCs) for medical research Recently, the Michael Davidson...Susanna Bachle and Matt Takvorian When COVID-19 researchers started working with us to help them share their...Transfer Offices at universities to make COVID-19 research-related plasmids available more broadly: to scientists...for-profit setting.  In light of the need for basic research during the current pandemic, universities are ... of support about allowing use of plasmids for research by anyone. As infection rates were increasing ...partners quickly assessed how to more readily enable research. Often “normal” timelines that could be weeks ...

...more into what researchers felt about NgAgo, I conducted a survey and asked researchers if they had tested...Update (November 18, 2016): Researchers from a variety of institutions recently reported their inability...sequence for target recognition, which gives a researcher unprecedented freedom to target any sequence ...form a complex with an ssDNA guide. Therefore, researchers must co-transfect cells with 5’-P-ssDNA guides... in the versatility of its delivery methods. Researchers can conveniently transfect/inject cells with ...requested by labs around the globe nearly 400 times. Researchers have been excitedly testing NgAgo's genome editing...discussions in the forums, it appeared that many researchers were having difficulty achieving indel formation...

... like many search engines, featuring both a general search bar tool and an advanced search option. For...   You can also search by protein sequence with BLAST, using the homepage search bar. Once you have...only Cas enzyme widely available and applied by researchers for gene targeting. Fast forward a decade, and...Institute. What is CasPEDIA? CasPEDIA is an online, searchable resource that provides summary information on...section on each enzyme page, you'll find a link to search Addgene's collection for plasmids containing that...  Helpful resources and information For some researchers, the previous information may be all you need...table of protein entry results. For the advanced search options, this is where you can really identify ...

...Balancing research with medical practice as an MD/PhD. Darrel does 20% clinical work 80% research and was... but later got obsessed with research. Chapter 3: How all basic research ends up being translational. ...for Regenerative Medicine accelerates research. Chapter 5: Research currently under way in the Kotton Lab...Chapter 8: The importance of a mix of research approaches. Why researchers should have a mix of high-risk, ...at Boston University Medical School. Darrell’s research focuses on the lungs, and, among other projects...Regenerative Medicine, and within the biomedical research community. Along these lines, Darrell recently...sharing. Listen to learn more about Darrell, his research, and his focus on open science. Listen to the...

...postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. It is commonly cited that...perform in vitro kinase reactions. Often, however, researchers lack knowledge of the regulatory kinase, access... phosphoserine (as described below) provides researchers with a valuable tool for understanding the contribution...curious anomaly amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA... phosphoserine (Sep)-tRNA synthetase enabled researchers to create an orthogonal translation system (OTS...encoding the ORF to be expressed Finally, the researcher needs a plasmid encoding their open reading frame...non-phospho-incorporated proteins purified from the system, researchers can run samples on SDS-PAGE gels containing Phos-Tag...

...has the sequence of the mutated gene the researcher is searching for), then the probe will bind to the sample...of DNA used saving the researcher both time and resources (3). Still, researchers generating transgenic...experiments allowed researchers to identify the location of genes. With this knowledge, researchers were able to...at the end as in standard PCR. This allows the researcher to compare different DNA sequences to see which...In the era before sequencing was commonplace, researchers used restriction mapping to investigate DNA sequence...running the products on an agarose gel allows a researcher to properly orient specific fragments of a DNA...This approach to studying DNA sequences helped researchers at a time when sequencing was still in the early...

...those by researchers hoping to increase community participation in new precision medicine research. A willingness...entails a lot of researching and writing – following the latest developments in genetics research, their applications...work in these fields of research, what often concerns them the most about research is its implications for...possibility of benefiting from the fruits of scientific research, and prevent technological advances from exacerbating...to dark episodes in the history of eugenics and research misconduct. Past ethical breaches such as the ...used to being very focused on narrowly-defined research questions in our field of interest. We often communicate...and awareness of issues outside of your field of research will allow you to better engage these diverse ...