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...path.   Yeast as a model organism Yeast are single cell, eukaryotic organisms formally classified as fungi...know that yeast is a powerful eukaryotic model organism – its genome is easy to manipulate, it’s affordable...cerevisiae (budding). Yeast make for an excellent model organism for a few reasons: Affordable to culture and...studies Peroxisomes are small, membrane-bound organelles essential for sequestering certain enzymes and... methanol metabolism occurs in methylotrophic organisms. K. phaffii is an excellent model for peroxisome...et al., Komagataella phaffi as Emerging Model Organisms in Fundamental Research. Front. Microbiol. 11-...Resources on the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening...

...Collection are sorted by organism (see screenshot above). Don’t see your favorite organism listed? Help us serve...us know about other organisms we should include by emailing us at help@addgene.org. If you are looking...plasmids made in your lab, see www.addgene.org/deposit or email deposit@addgene.org for more information or ...to narrow your keyword search to one of these organisms. Help us continue to develop resources for your...like to get the word out about a particular microorganism, microbial technique, or microbial technology...

...CRISPR-Cas systems. A full list of Acr proteins organized by the type of CRISPR-Cas system they block can...anti-CRISPR protein. Nature 546:436–439 . https://doi.org/10.1038/nature22377 Harrington LB, Doxzen KW, Ma... CRISPR-Cas9. Cell 170:1224-1233.e15 . https://doi.org/10.1016/j.cell.2017.07.037 Li C, Psatha N, Gil ...Methods & Clinical Development 9:390–401 . https://doi.org/10.1016/j.omtm.2018.04.008 Marino ND, Pinilla-Redondo...technologies. Nat Methods 17:471–479 . https://doi.org/10.1038/s41592-020-0771-6 Mayo-Muñoz D, He F, Jørgensen...islandicus Rod-Shaped Virus 2. Viruses 10:695 . https://doi.org/10.3390/v10120695 Pawluk A, Amrani N, Zhang Y, ...for CRISPR-Cas9. Cell 167:1829-1838.e9 . https://doi.org/10.1016/j.cell.2016.11.017 Shin J, Jiang F, Liu...

... ACS Chemical Neuroscience 6:476–484 . https://doi.org/10.1021/cn500325v Goutaudier, R., Coizet, V., Carcenac...Ligands. Eneuro, 6(5), ENEURO.0171-19.2019. https://doi.org/10.1523/ENEURO.0171-19.2019Jendryka M, Palchaudhuri...chemogenetics in mice. Scientific Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern ...mice. Nature Biotechnology 17:165–169 . https://doi.org/10.1038/6165 Roth BL (2016) DREADDs for Neuroscientists...Neuroscientists. Neuron 89:683–694 . https://doi.org/10.1016/j.neuron.2016.01.040 Saloman, J. L., Scheff, N... of Neuroscience, 36(42), 10769–10781. https://doi.org/10.1523/JNEUROSCI.3480-15.2016 Weston M, Kaserer...

...from organization to organization, so you’ll need to be familiar with your specific organization/department...-2 labs. When working with lentivirus, many organizations require or recommend disposable gowns over your...cabinet, even if it is not required by their organization. It’s always okay to add another level of protection..., 10% bleach, or 1% iodine are useful. Some organizations or cities may require a higher percentage of...cut. Following all sharps procedures for your organization will significantly reduce the risk of an accident...also need to familiarize yourself with your organization’s reporting mechanisms before you start an experiment.... Happy transducing! Resources Resources on Addgene.org Addgene viral services Addgene Biosafety Guide...

...to deliver a standard AdV vector to the targeted organs or tissues with Cas9 and a gRNA designed to target...Genome Editing. Circ Res 115:488–492 . https://doi.org/10.1161/circresaha.115.304351 Holkers M, Maggio... nucleases. Nat Methods 11:1051–1057 . https://doi.org/10.1038/nmeth.3075 Lukashev AN, Zamyatnin AA Jr...perspectives. Biochemistry Moscow 81:700–708 . https://doi.org/10.1134/s0006297916070063 Maddalo D, Manchado E...CRISPR/Cas9 system. Nature 516:423–427 . https://doi.org/10.1038/nature13902 Maggio I, Holkers M, Liu J,...diverse array of human cells. Sci Rep 4: . https://doi.org/10.1038/srep05105 Stephens CJ, Lauron EJ, Kashentseva...Journal of Controlled Release 298:128–141 . https://doi.org/10.1016/j.jconrel.2019.02.009 Wang D, Mou H, Li...

...CRISPR–Cas system. Nature 577:271–274 . https://doi.org/10.1038/s41586-019-1849-0 Klompe SE, Vo PLH, Halpin-Healy... DNA integration. Nature 571:219–225 . https://doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova KS...Proc Natl Acad Sci USA 114:E7358–E7366 . https://doi.org/10.1073/pnas.1709035114 Rubin BE, Diamond S, Cress...Within Microbial Communities. bioRxiv https://www.biorxiv.org/content/10.1101/2020.07.17.209189v2 Strecker...CRISPR-associated transposases. Science 365:48–53 . https://doi.org/10.1126/science.aax9181 Strecker J, Ladha A, Makarova... transposases.” Science 368:eabb2920 . https://doi.org/10.1126/science.abb2920 Vo PLH, Ronda C, Klompe...bacterial genome engineering. Nat Biotechnol. https://doi.org/10.1038/s41587-020-00745-y Zhang Y, Sun X, Wang...

...Why have zebrafish become such a popular model organism? Zebrafish (Figure 1) were initially recognized...reasons that zebrafish have been embraced as a model organism, I’ll outline a few of the available genetic resources...reason for the success of zebrafish as a model organism is its amenability to genetic manipulation. Tools...Europe, and China. In addition, the Zebrafish Model Organism Database ZFIN actively curates information on ...Generating zebrafish transgenics Creating transgenic organisms by introducing foreign DNA into the genome is ...fertilized zebrafish zygote transforms into an organism with a clearly established body plan including...pinhead—contribute to the “sculpting” of tissues and organs during embryo development. Single cell sequencing-based...

...blog posts about plasmid cloning Resources on Addgene.org Download the Plasmids 101 eBook  Browse plasmids...low-copy number plasmids. Plasmid 80:54–62 . https://doi.org/10.1016/j.plasmid.2015.03.007 Pubmed PMID: 25889267... Molecular Microbiology 56:1430–1440 . https://doi.org/10.1111/j.1365-2958.2005.04643.x Pubmed PMID: 15916596...plasmids in vivo. PLOS ONE 12:e0172913 . https://doi.org/10.1371/journal.pone.0172913 Pubmed PMID: 28245276...Plasmid Incompatibility. PLoS ONE 7:e29875 . https://doi.org/10.1371/journal.pone.0029875 Pubmed PMID: 22253811...Letters in Applied Microbiology 47:235–240 . https://doi.org/10.1111/j.1472-765x.2008.02426.x Pubmed PMID: 19241516...Opinion in Structural Biology 22:72–79 . https://doi.org/10.1016/j.sbi.2011.11.001 Pubmed PMID: 22153351...

... Resources and references More resources on addgene.org  Addgene's CRISPR Guide Addgene's CRISPR 101 ...Reviews Microbiology, 20(11), 641–656. https://doi.org/10.1038/s41579-022-00739-4 References Bravo, J....CRISPR–Cas9. Nature, 603(7900), 343–347. https://doi.org/10.1038/s41586-022-04470-1 Cofsky, J. C., Soczek...Structural & Molecular Biology, 29(4), 395–402. https://doi.org/10.1038/s41594-022-00756-0 Jiang, F., & Doudna,...Annual Review of Biophysics, 46, 505–529. https://doi.org/10.1146/annurev-biophys-062215-010822 Nishimasu...and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Osuka, S., Isomura, ...CRISPR–Cas9. The EMBO Journal, 37(10), e96941. https://doi.org/10.15252/embj.201796941 Pattanayak, V., Lin, S....

...investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An additional...mechanisms to maintain and grow these critical organizations should be a fundamental part of the scientific... study. The Lancet Microbe 1:e14–e23 . https://doi.org/10.1016/s2666-5247(20)30004-5 McCray PB Jr, Pewe...Syndrome Coronavirus. JVI 81:813–821 . https://doi.org/10.1128/jvi.02012-06 Ou X, Liu Y, Lei X, Li P, ...cross-reactivity with SARS-CoV. Nat Commun 11: . https://doi.org/10.1038/s41467-020-15562-9 Subbarao K, Roberts ...SARS? Trends in Microbiology 14:299–303 . https://doi.org/10.1016/j.tim.2006.05.007 (2020) Open for outbreaks...outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020-0499-y Additional resources on the...

... the effects of the drug on the actual cells or organ system. This allows scientists to get a more definitive...Patients. Frontiers in Immunology, 13. https://www.frontiersin.org/articles/10.3389/fimmu.2022.885672Piché, ...Assessment (pp. 519–542). Academic Press. https://doi.org/10.1016/B978-0-12-417144-2.00027-5van Brummelen...Challenges. The oncologist, 21(10), 1260–1268. https://doi.org/10.1634/theoncologist.2016-0061WHO. International...Frontiers in drug discovery, 2, 952326. https://doi.org/10.3389/fddsv.2022.952326Joubert, M. K., Deshpande...Biotherapeutics. PloS one, 11(8), e0159328. https://doi.org/10.1371/journal.pone.0159328Groell, F., Jordan,...Pharmazeutische Verfahrenstechnik e.V, 130, 128–142. https://doi.org/10.1016/j.ejpb.2018.06.008Lu, RM., Hwang, YC., ...

...prime editors. Nat Biotechnol 38:824–844 . https://doi.org/10.1038/s41587-020-0561-9 Dempster JM, Pacini C...dependency data sets. Nat Commun 10: . https://doi.org/10.1038/s41467-019-13805-y Doench JG (2017) Am ...genetic screens. Nat Rev Genet 19:67–80 . https://doi.org/10.1038/nrg.2017.97 Hanna RE, Doench JG (2020) ...experiments. Nat Biotechnol 38:813–823 . https://doi.org/10.1038/s41587-020-0490-7 Radzisheuskaya A, Shlyueva...repression. Nucleic Acids Res 44:e141–e141 . https://doi.org/10.1093/nar/gkw583 Rees HA, Liu DR (2018) Base ...living cells. Nat Rev Genet 19:770–788 . https://doi.org/10.1038/s41576-018-0059-1 Veres A, Gosis BS, Ding... Sequencing. Cell Stem Cell 15:27–30 . https://doi.org/10.1016/j.stem.2014.04.020 Yang L, Guell M, Byrne...

...paper or preparing a presentation, it's easy to forget that you have a much bigger job than merely telling... at Addgene, ComSciCon, and many more great organizations. At ComSciCon, our primary mission is to strengthen...respective fields. To help attendees launch new organizations that will extend opportunities to practice science...education initiatives like BiteScis (https://bitescis.org/) and amazing graduate student writing collaboratives...collaboratives like Oceanbites (https://oceanbites.org/) have already grown out of our workshops. If you'... create them.  Seek out collaborations with organizations that practice science communication or start...Facebook, Instragram, or send an email to info@comscicon.org. Many thanks to our guest blogger, Nathan Sanders...

...https://doi.org/10.7554/eLife.64631  Goertsen D, et al. Nature Neuroscience. 2022. https://doi.org/10.1038...proteomic mapping in both cell culture and whole organisms (see our 2019 overview). Until recently, the approach...dedicated transgenic line. Using zebrafish as a model organism, the Parton Lab recently created a flexible system...Given that the liver is an immunologically active organ, this advance is likely to afford the ability to...Goertsen D, et al. Nature Neuroscience. 2022. https://doi.org/10.1038/s41593-021-00969-4.   dCas9-SSAP with ...here! Wang et al. Nat Cell Biol. 2022. https://doi.org/10.1038/s41556-021-00836-1 Visual barcodes for...here! Kaufman, et al. 2022. Nature Comm.https://doi.org/10.1038/s41467-022-30008-0      ...