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We narrowed to 842 results for: NTS

Showing: 391 - 400 of 842 results

  1. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...’ve got you covered. Defining your control requirements To pick the best tool for the job, it’s important...important to know what the job requirements are. Here are the questions you should be asking. What kind ...with the systems discussed above, just with more elements of control. Tet promoters Tetracycline-dependent...promoters are engineered by placing tet response elements upstream of a minimal promoter. These systems ...expression is tunable by titration with different amounts of tetracycline. Tetracycline regulation is reversible...

  2. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...Accelerate Research Discoveries) Challenge provides grants for rare disease research. This year is our fourth...candidate genes in euploid iPSCs. “The BeHEARD Addgene reagents will facilitate our aims to correct X chromosome.... So far, there have been thirty GNB1 missense variants identified from 58 individuals with GNB1 disorder...biosensors and iPSC-derived cortical neurons from patients with GNB1 mutations, the lab hopes to capture ...upon stimulation. “To study cellular transduction events, we will utilize BRET-, FRET-, and intensity-based...

  3. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ... makes polyclonal antibodies unique and what experiments you should choose them for. How polyclonal antibodies...Pre-adsorbed polyclonal antibodies are designed for experiments where antibodies raised in several different ...antibodies (which recognize other antibodies) in experiments that require multiple antibodies that each detect... For the same reason, they’re also useful in experiments where the availability of some regions of the...want to use the same positive control across experiments to be aware of signal strength. In general, polyclonal...

  4. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...based on their audience. How you talk about your experiments to your PI, your colleagues, or your non-scientist... other backgrounds at conferences or networking events. And if you’re a wet lab scientist who works frequently...formal definitions in our lexicon. We created documents and presentations, gave real life examples of ...documentation, including SOPs or project-related documents, we define new and potentially ambiguous terms... and asking can help the team identify possible points of confusion in the future. Improving cross team...

  5. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...turnover is radioactive pulse-chase experiments. However, these experiments are problematic because they require...annual symposium is organized by international PhD students at the Max Planck Institute for Biophysical Chemistry...MPI in Frankfurt am Main and perform similar experiments. Visualizing newly synthesized proteins with ...microscope she likes to travel, discover new restaurants, go to concerts and bake. you can contact her...

  6. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...Stanford University. Sex and gender are critical components of biological research that are often forgotten...discovery. Tackling sex and gender in research Governments, universities, and, increasingly, corporations...researchers consider the sex of their cells when doing experiments—which can lead to failed research. For example...meant that, by the very nature of the way the experiments were performed, they did not see anything unique...of human clinical trials. And, as Janine Clayton points out, late-stage clinical studies that draw from...

  7. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...From FPs optimized for oxidizing environments to photoconvertible variants, it seems like GFP is always learning... found suitable pairs, they constructed three components: GBPa-VP16 (activation domain); GBPb-GAL4 (DNA-binding...possibility of adapting GFP lines for optogenetics experiments in specific cell populations. Listen to Our Podcast...proteins with a modular structure, constructing split variants should be relatively straightforward, but it is...

  8. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...rely on fusion proteins while others re-engineer components of Cas systems themselves. SunTag, SAM, and VPR... VPR have all shown significant improvements upon the initial dCas9-VP64 method, so there are multiple...much higher activation, dCas9-VP64 is great for experiments that requires modest gene activation. Synergistic...making it a popular method for gene activation experiments. In cases of multiplex gene regulation (activating...lines (Sajwan et al., 2019). SPH SPH combines components of SAM and SunTag activators, fusing the epitope...

  9. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... that allows for the assembly of multiple DNA fragments efficiently and accurately in a “single pot.’ ...quantification of this depletion using imaging-based measurements (Ashley et al., 2021).   Schematic showing...Resources page.  Plasmids for delivering CRISPRi components by a single AAV vector are now available from...motif (PAM). They're used to determine the PAM requirements of Cas enzymes. CRISPR-ARE is a new system from.... Check out the new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f, now...

  10. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...sure that what we observe through the microscope represents reality as accurately as possible. This is especially...discover more resources for your genomic tagging experiments. Browse the Allen Institute for Cell Science ...of the gene of interest. To get the molecular components of CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA...destabilizes the cell’s outer membrane and allows the components to physically enter the cells so they can go ...easier for you to use genomic tagging in your experiments. Many thanks to our guest bloggers from the ...
Showing: 391 - 400 of 842 results