We narrowed to 803 results for: TIM

1. Your Lentiviral Plasmid FAQs Answered

   Type

   Blog Post

   Published

   April 23, 2014, 1:08 p.m.

   ... that a construct is functional before investing time and resources into making a stable cell line. When...

2. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

   Type

   Blog Post

   Published

   Sept. 13, 2018, 1:55 p.m.

   ...libraries Overview of Library To better define the optimal conditions for CRISPR knockout screens, the Bassik...

3. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ...episomal vector system generated by the group is an optimized mixture of three episomal plasmids (Table 1) encoding...

4. CRISPR Activation: A Practical Guide

   Type

   Blog Post

   Published

   Aug. 18, 2016, 2:30 p.m.

   ...appropriate isoform(s) of a given gene, as, many times, the isoform(s) common to a particular cell type...

5. Viral Vectors 101: Pseudotyping

   Type

   Blog Post

   Published

   April 20, 2021, 2:30 p.m.

   ...by nonspecific complement mechanisms in serum. Sometimes this is due to the cells that the viral vectors...

6. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

   Type

   Blog Post

   Published

   July 23, 2019, 12:46 p.m.

   ...peripheral nervous system (PNS) targeting AAV  Get 1st time AAV user tips from an AAV rookie  Learn about some...

7. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

   Type

   Blog Post

   Published

   April 2, 2019, 1:44 p.m.

   ...and Bagpipe. How does a gene get its name? Often times, a gene gets its name based on what happens to an...

8. Plasmids 101: Gateway Cloning

   Type

   Blog Post

   Published

   Jan. 12, 2017, 3:30 p.m.

   ...that will work well with a given set of resources, time, and experimental needs. Since its invention in ...

9. Plasmids 101: Shuttle Vectors

   Type

   Blog Post

   Published

   May 20, 2025, 1:15 p.m.

   ...vectors typically involves shuttle vectors, also sometimes called transfer vectors. The gene of interest ...

10. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...lentiviral minus strand in the 3’ LTR. These optimizations produced 90% accurate integration of multiplexed...

11. Antibodies 101: Reading a Flow Plot

    Type

    Blog Post

    Published

    Feb. 6, 2024, 3:13 p.m.

    ...annotated like so: CD14high or CD14low.  Don’t be intimated if you’re struggling to understand how the researchers...

12. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    Published

    Nov. 19, 2020, 2:15 p.m.

    ...Gail Mandel and her team have shown for the first time that programmable RNA editing can repair a gene ...

13. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ...experiments. They’ve also helped researchers design and optimize cloning methodologies using counterselection. ...

14. Adeno-associated Viruses (AAVs) for Genome Editing

    Type

    Blog Post

    Published

    March 27, 2018, 1:32 p.m.

    ...in vivo applications.  Third, even in the most optimized tissue-culture based system, the efficiency of...

15. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    Published

    May 10, 2016, 2:30 p.m.

    ...genomes will need to be sequenced to determine the optimal gRNAs for either “shock and kill” or viral excision...

16. Plasmids 101: Biotinylation

    Type

    Blog Post

    Published

    Nov. 15, 2018, 1:50 p.m.

    ...(McInnes et al., 1990). Photobiotinylation is sometimes easier and less expensive than other enzymatic...

17. Plasmids 101: Expressing shRNAs for RNA Interference

    Type

    Blog Post

    Published

    June 17, 2025, 1:15 p.m.

    ...dedicated field of study for improving these tools. Next time you need to knock down a gene, maybe you’ll consider...

18. Antibodies 101: Beyond Surface Labeling

    Type

    Blog Post

    Published

    June 27, 2024, 1:15 p.m.

    ...using biorender.com.   Intracellular labeling Oftentimes, a marker of interest is not located on the cell...

19. Plasmids 101: Common Lab E. coli Strains

    Type

    Blog Post

    Published

    Nov. 7, 2014, 2:56 p.m.

    ...designing your plasmid – you carefully selected the optimal promoter for your gene of interest, painstakingly...

20. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    Published

    July 12, 2016, 2:30 p.m.

    ...CRISPR is easier than more conventional methods. Time and money savings come from the fact that CRISPR...