We narrowed to 816 results for: ache
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TypeBlog PostPublishedJune 26, 2018, 1:33 p.m...., Michael McMurray, and Richard Gronostajski who each are being awarded free plasmids form Addgene to ...
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Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....dependent on the ability of agonists to successfully reach target cells while resulting in minimal off target... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....genes that help bacteria invade hosts, including attaching to host cells and evading the host immune system... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog PostPublishedNov. 7, 2017, 1:59 p.m....back into the infants. Almost all patients have achieved persistent immunological reconstitution with a... -
6 Tips for Grant Writing
TypeBlog PostPublishedOct. 18, 2016, 2:30 p.m....technologies to solve a problem can allow you to approach that problem in a novel way. Finally, engineering... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog PostPublishedJuly 14, 2016, 2:30 p.m....LbCpf1 (blue). Mismatch positions are indicated below each graph. Figure adapted from Kleinstiver et al., 2016... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m....funded on a membership model - everyone contributes each month - with only occasional supplementation with... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog PostPublishedAug. 29, 2019, 12:50 p.m....background without an introduction. If someone approaches your poster, introduce yourself and ask about... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....glycol (PEG). We add 25 mL of 40% PEG solution to each 100 mL of supernatant (final concentration of 8%... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m....homology arm repair templates containing the tag for each insertion. Also, it remains challenging to screen... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....heterozygote, and the mutation won’t drift away with each passing generation. Another feature of balancer... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....et al. (2015). “A Scalable Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human Cells... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....two types of languages: one to communicate amongst each other, like this conversation we are having now ... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m....previous layout, the number of plasmids matching each filter is displayed in parentheses. In this example... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog PostPublishedMay 30, 2019, 12:44 p.m....and retroviral expression system for using this approach in in vivo studies (Fujita et al., 2018). In addition... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m.... in bacterial, yeast, plant, or mammalian cells. Each of these systems has their advantages and disadvantages... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....treatment of a molecular disorder: gene therapy approaches to sickle cell disease. Hoban MD, Orkin SH, Bauer... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....SIN is shorthand for self-inactivating, which is achieved by deleting a large portion of the 3'LTR in the... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....you show respect for your own career and it will teach them to respect their own responsibilities. Another... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m.... are 4 bases (A, T, C, and G) and 3 positions in each codon. There are therefore 64 possible codons but...