We narrowed to 817 results for: nin
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TypeBlog PostPublishedJan. 31, 2019, 1:32 p.m..... PubMed PMID: 15261139. Barsky, Sanford H., and Nina J. Karlin. "Myoepithelial cells: autocrine and paracrine...
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CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....CRISPRainbow paper, Ma et al. engineered gRNA scaffolds containing two hairpin sequences, which recruit fluorescent... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog PostPublishedMay 5, 2015, 3:54 p.m.... papers is to practice reading them. There’s a learning curve for the jargon and background in your field... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....there’s a lot of control spatially as to what’s happening at different points within the wood. There are... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 1, 2018, 1:57 p.m....lines and these cells are then grown in biotin-containing media. FRET is another example of a protein fragmentation... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....receive bacteria through the mail due to laws concerning genetically modified organisms. Since Addgene... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog PostPublishedAug. 6, 2019, 12:55 p.m....alleles, such as Cre, function when hemizygous, meaning that only one chromosome needs to contain the transgene... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....Central PMCID: PMC4523203. Many empty vectors containing these fluorescent proteins are available on our... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 3, 2022, 1:15 p.m....single-molecule tracking in live cells. Controls When designing experiments, it’s important to include proper ... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....mixture is added to the wells. Any free antibody (meaning it hasn’t bound to your protein of interest) will... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog PostPublishedJan. 9, 2020, 1:45 p.m....analyzes publications and figures using machine learning to extract important features such as influential... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog PostPublishedSept. 13, 2016, 2:30 p.m....departments across the university. Collective bargaining may provide a better deal for students in areas... -
Tips to Make the Most of a Scientific Conference
TypeBlog PostPublishedMay 23, 2019, 12:26 p.m....Twitter, for instance. If you’ve had a particularly meaningful conversation, and you have their email address... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....tags we will discuss here are all conditional, meaning that they only facilitate degradation of their ... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....specific antibody to the C-terminal five amino acids, meaning the tag could be quite small. However, substance... -
PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....occurs in the few months leading up to applications opening. With virtual options, you can narrow down your... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....Knockouts with CRISPR through Non-Homologous End Joining Learn How the FLEx Switch Can Be Used to Turn Genes... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m..... Cas9 guide RNA delivery does however rely on cloning into a vector or in vitro synthesis, which could... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....PMCID: PMC2173526. 2. Zacharias, D.A., et al., Partitioning of lipid-modified monomeric GFPs into membrane... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....Stability: This parameter is important if you are planning to express the FP in acidic environments (e.g....