We narrowed to 821 results for: nin

1. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

   Type

   Blog Post

   Published

   Jan. 31, 2019, 1:32 p.m.

   .... PubMed PMID: 15261139. Barsky, Sanford H., and Nina J. Karlin. "Myoepithelial cells: autocrine and paracrine...

2. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   Published

   April 23, 2018, 2 p.m.

   ...and Schuh labs. Clift et al. applied TRIM-away to nine proteins in ten ddifferent cell types, including...

3. Human Germline Editing Using CRISPR

   Type

   Blog Post

   Published

   Aug. 10, 2017, 2:19 p.m.

   ...and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated...

4. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   Published

   June 15, 2017, 2:30 p.m.

   ...an affinity of 110 µM (Zacharias et al, 2002), meaning that at this concentration 50% of the protein forms...

5. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

   Type

   Blog Post

   Published

   April 13, 2017, 2:30 p.m.

   ...easy to identify: double-membraned structures containing undigested cytoplasmic contents. Autophagosomes...

6. CRISPRainbow and Genome Visualization

   Type

   Blog Post

   Published

   Feb. 28, 2017, 3:30 p.m.

   ...CRISPRainbow paper, Ma et al. engineered gRNA scaffolds containing two hairpin sequences, which recruit fluorescent...

7. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

   Type

   Blog Post

   Published

   May 5, 2015, 3:54 p.m.

   ... papers is to practice reading them. There’s a learning curve for the jargon and background in your field...

8. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ...there’s a lot of control spatially as to what’s happening at different points within the wood. There are...

9. Mouse Modeling, Part 2: Breeding and Crossing Mice

   Type

   Blog Post

   Published

   Aug. 6, 2019, 12:55 p.m.

   ...alleles, such as Cre, function when hemizygous, meaning that only one chromosome needs to contain the transgene...

10. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    Published

    Aug. 24, 2021, 1:15 p.m.

    ...mixture is added to the wells. Any free antibody (meaning it hasn’t bound to your protein of interest) will...

11. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 3, 2022, 1:15 p.m.

    ...single-molecule tracking in live cells.  Controls  When designing experiments, it’s important to include proper ...

12. Early Career Researcher Toolbox: Finding Relevant Papers

    Type

    Blog Post

    Published

    Jan. 9, 2020, 1:45 p.m.

    ...analyzes publications and figures using machine learning to extract important features such as influential...

13. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 1, 2018, 1:57 p.m.

    ...lines and these cells are then grown in biotin-containing media. FRET is another example of a protein fragmentation...

14. Lab Automation at Addgene

    Type

    Blog Post

    Published

    March 22, 2018, 1:28 p.m.

    ...receive bacteria through the mail due to laws concerning genetically modified organisms. Since Addgene...

15. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    Published

    June 27, 2017, 1:03 p.m.

    ...Central PMCID: PMC4523203. Many empty vectors containing these fluorescent proteins are available on our...

16. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type

    Blog Post

    Published

    Sept. 13, 2016, 2:30 p.m.

    ...departments across the university. Collective bargaining may provide a better deal for students in areas...

17. Tips to Make the Most of a Scientific Conference

    Type

    Blog Post

    Published

    May 23, 2019, 12:26 p.m.

    ...Twitter, for instance. If you’ve had a particularly meaningful conversation, and you have their email address...

18. Of Myc and Men

    Type

    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ...specific antibody to the C-terminal five amino acids, meaning the tag could be quite small. However, substance...

19. PhD Applications After COVID

    Type

    Blog Post

    Published

    Jan. 5, 2023, 2:15 p.m.

    ...occurs in the few months leading up to applications opening. With virtual options, you can narrow down your...

20. Plasmids 101: Degron Tags

    Type

    Blog Post

    Published

    March 14, 2024, 1:15 p.m.

    ...tags we will discuss here are all conditional, meaning that they only facilitate degradation of their ...