We narrowed to 837 results for: Eras
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TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....channel in 2013, which continue to grow today. “I generally refer people to Addgene for the basics of molecular...
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Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....it takes, ooh, approximately the length of your average PhD to achieve!) Each field has its own framework... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....interest. However, we also found that this is not generalizable to all fusion proteins. For example, we saw ... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....Label your tubes with a label maker. Label makers generate far more legible labels than your hand and help... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....MicroPublication 1 (2018): 2–3. Wang, Han, et al. "cGAL, a temperature-robust GAL4–UAS system for Caenorhabditis elegans... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....can spread between hosts through horizontal (or “lateral”) gene transfer. Horizontal gene transfer can occur... -
Viral Vectors 101: Calcium Sensors
TypeBlog PostPublishedAug. 10, 2023, 1 p.m....calcium sensors for in vivo studies) There are several calcium reporters specially designed for the aims... -
Design Tips for Prime Editing
TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m....and goal of the experiment. Now, onto the tips! General principles pegRNA design If you’re just starting... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....homologies. Abbreviated protocol for PITCh Step 1: Generate microhomologies in the PITCh vector ~20 bp 5’... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....Retroviral pEF1a-IRES-Neo IRES Mammalian MSCV-IRES-Luciferase IRES Retroviral pWPI IRES Lentiviral AmCyan-P2A-mCherry... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m.... PARIS, a zinc finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....you probably need to find a new job. If you are miserable at home or work, your relationships will suffer... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m....of protein known as inclusion bodies that will generally segregate with the cell pellet during purification... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....experiment? Not quite. Shuttle vectors have to address several big differences in the ways vectors are used across... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog PostPublishedApril 8, 2025, 1:15 p.m....setup. We recommend starting by searching the literature to see what is already known about your housekeeping... -
10 Steps to a Perfect Science Talk
TypeBlog PostPublishedAug. 23, 2016, 2:30 p.m.... care about these details, but does follow the general structure and flow of the entire session. I myself... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m....Excitation Laser Color Very Bright Bright Moderate Dim Ultraviolet (355 nm) BD Horizon...