We narrowed to 872 results for: ica;

1. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

   Type

   Blog Post

   Published

   Nov. 29, 2018, 1:53 p.m.

   ...of dsDNA and RNA in trans. Fluorescent signal indicates the presence of this viral sequence in the assay...

2. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

   Type

   Blog Post

   Published

   July 26, 2018, 12:46 p.m.

   ...KTRs) Kinase Translocation Reporters (KTRs) are genetically encoded fluorescent kinase reporters. A KTR is...

3. Stepping into a Virtual Internship: My Experience at Addgene

   Type

   Blog Post

   Published

   Aug. 13, 2020, 1:21 p.m.

   ...right path. The frequent Zoom meetings helped me communicate my ideas. I was able to express my visions for...

4. Our Most Popular Blog Posts from 2019

   Type

   Blog Post

   Published

   Dec. 31, 2019, 2:26 p.m.

   ...Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley,...

5. Six Spooky Science Stories and Halloween at Addgene

   Type

   Blog Post

   Published

   Oct. 31, 2019, 1:06 p.m.

   ...approach." Proceedings of the Royal Society B: Biological Sciences 272.1577 (2005): 2117-2126. PubMed PMID...

6. Inside Addgene's 10 Year Anniversary Gala

   Type

   Blog Post

   Published

   July 3, 2014, 3:48 p.m.

   ...course of scientific research. What was once a complicated process of requesting and sharing plasmids has...

7. CASTing Off for New Shores in Human Genome Editing

   Type

   Blog Post

   Published

   June 10, 2025, 1:15 p.m.

   ...National Academy of Sciences of the United States of America, 114(35), E7358–E7366. https://doi.org/10.1073/...

8. Bringing Sustainable Practices to the Lab: Recycling

   Type

   Blog Post

   Published

   Feb. 14, 2019, 2:35 p.m.

   ...easier to ship back. Be that person! If you use serological pipettes from Corning®, Falcon®, or Axygen®, ...

9. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

   Type

   Blog Post

   Published

   March 15, 2018, 12:59 p.m.

   ... a Cas9 nickase and two gRNAs.  For nickase applications, a common question is: how should the gRNAs ...

10. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type

    Blog Post

    Published

    Dec. 12, 2017, 3:13 p.m.

    ...providing an avenue for temporary/reversible CRISPR modification. In addition to these subpages, our updated ...

11. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type

    Blog Post

    Published

    Aug. 22, 2019, 12:43 p.m.

    ...complements the auxotrophy (i.e., synthesizes the critical amino acid). Many common E. coli strains used ...

12. Some Like it Hot: Thermostable GeoCas9

    Type

    Blog Post

    Published

    Sept. 14, 2017, 12:40 p.m.

    ...could also be used with in vitro molecular bio applications that require high temperature reaction conditions...

13. Management for Scientists: Giving Feedback

    Type

    Blog Post

    Published

    Feb. 6, 2015, 2:46 p.m.

    ... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...

14. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type

    Blog Post

    Published

    June 22, 2021, 1:15 p.m.

    ...coronavirus and SARS-CoV. These two viruses are quite genetically distinct from SARS-CoV-2 and its variants. Yet...

15. New and Upcoming Viral Vectors - December 2019

    Type

    Blog Post

    Published

    Dec. 19, 2019, 2:40 p.m.

    ...need to determine these transduction patterns empirically for their own experiments. To this end, we offer...

16. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type

    Blog Post

    Published

    Sept. 17, 2020, 1:15 p.m.

    ...requests for scientists doing almost every type of biomedical science research. Our work accelerates and enhances...

17. Components of CRISPR/Cas9

    Type

    Blog Post

    Published

    Feb. 2, 2016, 5 p.m.

    ... to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist...

18. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type

    Blog Post

    Published

    Oct. 9, 2014, 3 p.m.

    ...month, deciding on the best protein for a given application is a challenge. To help keep you abreast of the...

19. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type

    Blog Post

    Published

    Sept. 26, 2024, 1:15 p.m.

    ... this limitation, one option is to perform a hierarchical assembly, assembling fragments in multiple steps...

20. Multiplex Genome Editing with CRISPR-Cpf1

    Type

    Blog Post

    Published

    May 9, 2017, 2:12 p.m.

    ... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...