We narrowed to 851 results for: nts

1. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

   Type

   Blog Post

   Published

   Sept. 13, 2018, 1:55 p.m.

   ...due to both on- and off-target effects, but also presents a strategy to control for these effects. By testing...

2. Hot Plasmids: Fall 2024

   Type

   Blog Post

   Published

   Nov. 21, 2024, 1:30 p.m.

   ...using mCherry for selection of sgRNA plasmid transformants, they avoid M. abscessus’ high rate of spontaneous...

3. Twenty Years of Sharing: Addgene's Viral Vector Service

   Type

   Blog Post

   Published

   July 30, 2024, 1:15 p.m.

   ...could be a matter of the size of the incremental improvements between generations: jGCaMP8 allows for the ...

4. Viral Vectors 101: Pseudotyping

   Type

   Blog Post

   Published

   April 20, 2021, 2:30 p.m.

   ...King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV...

5. Plasmids 101: E. coli Strains for Protein Expression

   Type

   Blog Post

   Published

   Feb. 10, 2015, 3:06 p.m.

   ...Although the DE3/T7 RNAP system works well for most experiments, the lac promoter can “leak,” meaning that a ...

6. Five Popular Model Organisms

   Type

   Blog Post

   Published

   April 11, 2019, 12:39 p.m.

   ...hermaphrodites making it easy to conduct large experiments over multiple generations. Similarly to other...

7. Quick Guide to Near-Infrared Fluorescent Proteins

   Type

   Blog Post

   Published

   Oct. 5, 2021, 1:15 p.m.

   ...biosensors NIR reporters can tell us about cellular events via  fluorescence changes occurring due to protein...

8. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   Published

   June 27, 2024, 1:15 p.m.

   ...surface of the CD8+ T cells and gate on the positive events for your flow analysis. However, certain downstream...

9. Plasmids 101: Common Lab E. coli Strains

   Type

   Blog Post

   Published

   Nov. 7, 2014, 2:56 p.m.

   ...strains, especially mutations that make major improvements such as those that increase plasmid yield and...

10. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    Published

    Jan. 16, 2025, 2:15 p.m.

    ... cell types. Figure 1: Diagram of AAV components. Created with BioRender.com. AAV vectors ...

11. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    Published

    Nov. 6, 2018, 1:12 p.m.

    ...directed differentiation.” Specific cell media, supplements, bioactive small molecules, and growth factors...