...provided by the Rinehart lab. Steinfeld et al., ACS Chem Biol 2014 May 16;9(5):1104-12. Heinemann et... your own designer bacteria. St-Pierre et al., ACS Synth Biol 2013 Sep 20;2(9):537-41. DREADD-based... One. 2011. Feb 18;6(2):e16765. Engler et al., ACS Synth Biol 2014. Feb 5 (Web); DOI: 10.1021/sb4001504...endogenous protein of interest and co-expressing its replacement recombinant protein with a FKBP domain and a ...tyrosine kinases (RTKs) are a large class of cell-surface receptors which play a critical role in development...dimerize on exposure to light, bringing the intracellular kinase domains into contact and initiating signaling...optimal scaffold can depend on the length of the spacer region between the target sequences. These updates...

...plasmids from Aranda-Díaz et al. Aranda-Díaz, et al. ACS Synth Biol. 2017. Pubmed PMID: 27930885. ...or noise, in reporter expression. To do so, one places reporter expression under the control of two separate...

...Wingler L, Pascolutti R, Paulo JA, Gygi SP, Kruse AC (2017) Multidimensional Tracking of GPCR Signaling...is initiated when a ligand binds to the extracellular surface of the GPCR. This results in a conformational...induce or inhibit intracellular signaling cascades as a response to the extracellular stimuli. Ligand dissociation...coupled with heterotrimeric G proteins on the intracellular side of the cell membrane. Heterotrimeric G ...relatively insensitive to their endogenous ligand, acetylcholine, and are only activated upon binding of CNO ...

... the decaffeination plasmid here. Quandt et al. ACS Synth Biol. 2013. PubMed PMID: 23654268. FLINC...OptoSTIM1), caused two-fold faster changes in intracellular Ca2+ levels than the original version containing...substrate, inducing a conformational change that placesTagRFP-T and Dronpa in close proximity. By monitoring...

...821. doi:10.1126/science.1225829  Hazan J, Bester AC. CRISPR-Based Approaches for the High-Throughput ...with multiple gRNAs poses the risk of disrupting adjacent genes, especially those regulated by or overlapping...al. 2019). The interactions between ncRNAs and adjacent genes are complex and not yet fully understood...

...Receptors Exclusively Activated by Designer Drugs. ACS Chemical Neuroscience 6:476–484 . https://doi.org...nonnative ligands. However, they retain their intracellular interactions and still set off the same pathways...

...editing toolbox.  References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR...in the genomic DNA to contain a specific protospacer adjacent motif (PAM) made up of 5′-NGG-3′ (“N” can...eighth round of ABE evolution and the “x” is the placeholder for the number that identifies the mutation in...to ABE7.10. Depending on the position in the protospacer, the editing capacity of the ABE8s ranged from...To expand the targeting scope of ABE8, the team replaced S. pyogenes Cas9 in the ABE complex with PAM-variant...

...retain some ability to induce Cas9 to make edits (AC, WLC, and JQ, unpublished results). An additional...that is complementary to the target site, or protospacer, in the genome. Native Cas9 has two catalytic...which cleaves one strand of DNA upon binding the protospacer. The resulting double strand break (DSB) stimulates...

...Jones, Nicola J. Patron, and Sylvestre Marillonnet. ACS Synth. Biol., Just Accepted Manuscript, DOI: 10.1021...

...References and resources References Bertiaux E, Balestra AC, Bournonville L, et al (2021) Expansion microscopy...?  ExM provides a straightforward answer to the space problem. Instead of imaging smaller, what if we ...