...image to the right). Once on the “Filters” page, select the category “LinkOut” and search for “Addgene”...Addgene Nonprofit plasmid repository” choice. This selection should populate your “PubMed filter list” to the...

...negative data. We want to make sure people are selecting AAVs that will work for their experiments, and...viral prep. And if you've used the Data Hub to help select an AAV for your experiments, let us know (datahub...

...in the same place, making it easier to go from selection to viral vector. To start the AAV packaged on ...a banner below the ordering table. Click on it, select your desired serotype and volume, and submit your...

...can help researchers save time and money when selecting an antibody! We encourage everyone interested ...Hub Antibodies 101: Validation Antibodies 101: Selecting the Right Antibody...

...function. Using bioinformatic analyses, they also selected for other key properties, notably high solubility...antibody. From their initial pool of candidates, they selected 12 candidate protein domains from non-vertebrate...

...type. Find some transduction characteristics of select AAV serotypes in Table 2 from the paper. Delivery... given in Tables 3-7. 3. Making use of AAV’s selectivity for neurons: targeting defined neuronal populations...

...and Linux. Inkscape is versatile and has a wide selection of tools for drawing and editing shapes and like...to particular types of images. For example, by selecting “Tools” and then clicking the “Usage rights” dropdown...

...recent months: Our selection of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use...

...fluorescent protein or something species-specific) or a selectable marker. Each insertion line can then be isolated...Transposons in the Lab Plasmids 101: Screens vs. Selections Plasmids 101: Knockout /Knock-In Plasmids ...

...were developed into 16 variants with different selection markers (blasticidin, neomycin, puromycin, hygromycin...fused to a region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool...

...Curie in Paris, has developed the retention using selecting hooks (RUSH) system. How does it work? RUSH is...PEST2D sequences. Several pre-constructed promoter-selection marker or promoter-inducible expression related...proteins (FPs). A subpopulation of mitochondria is selectively labeled by irradiating the photoactivatable FP...PAGE and then staining the gel with DHFBI to selectively visualize the Broccoli-tagged RNA. This technique...

...have different epitopes available if it is being selected out of a complex mix of proteins that have been...condition or configuration.  Validation  Once you’ve selected an antibody, or multiple antibodies, you’ll need...

... the lentiviral backbone to encode the CD90.1 selection marker, Thy1.1, in addition to puromycin resistance...New GRAB-DA and 5HT sensors New AAV tools to selectively control parvalbumin interneurons from the Dimidschstein...

... genome) and the other contains the bacterial selection marker and origin of replication. During bacterial...saving a glycerol stock to ensure the colony you selected contains the full plasmid. A diagnostic digest...

...sequence to be amplified (usually 18-21bp) When selecting restriction sites, you should use a DNA analysis... longer the expected PCR product is. You should select an annealing temperature based on the melting temperature...

...multiplexing. It is imperative that you use narrowly selective isotype-specific secondary antibodies when multiplexing...Antibodies 101: Polyclonal Antibodies Antibodies 101: Selecting the Right Antibody  ...

...of spending time and money producing virus from select vectors in the repository yourself, you can now...Cas9 nickase, Cas9 activators, Cas9 repressors, select gRNAs, and CRISPR pooled libraries. Whether you...

...more selective for different ions. Right now, we can modulate electricity. If you could selectively let...

...markers into a target genome. They did this by selecting the important elements from existing transposon...  A new tool for CRISPR gRNA validation Selecting a gRNA sequence that effectively targets your ...sequence or gene of interest into the pCoofy vectors, select the appropriate pair of vector and gene primers...

...terms in a search bar, by ticking some boxes, by selecting a suggestion — how should these be supported and...viral prep filter for promoter “Syn”, that, when selected, will match variations present in the data such...

...2018 Conference. I don’t exactly know how I was selected to participate on this panel, but my consistent...social media to their professional advantage, I’ve selected five resources that have tips for using social...

...difficult for individual labs to make efficiently. For select viral vectors in the repository, you can instead...in the brain, mapping neuronal networks, and selectively activating signaling pathways. Many scientists...

... contain the RP4 transfer origin as well as a selectable marker and a desired genetic payload (ex: GFP...sorted using FACS and isolated with antibiotic selection. The team tested this system in vitro and in vivo...

...Chemical Biology Find the AsCas12f1 plasmids   Selective endogenous encapsidation for cellular delivery...sequences in the capsid, in an approach called Selective Endogenous eNcapsidation for cellular Delivery...

...production of monoclonal antibodies starts with selecting just one of these B cell clones for further antibody...vials of the same lot of polyclonal antibody, or select a monoclonal or recombinant antibody instead. Otherwise...

... or Cre dependent gene switches, and serotype selection. Promoters and serotype control, respectively,...AAVs down, you’re ready to take the next step – selecting your AAV and AAV components (serotype and promoter...

...substrates such as Ni2+ and are therefore more selective. When using systems with higher levels of histidine-containing...production systems, consider using these more selective substrates along with stringent wash conditions...

... gRNA. These gRNAs can be cleaved and rendered useless after less than a minute of exposure to the appropriate...CRISPR resources Request ready-to-use viral preps of select CRISPR lentiviral plasmids Additional resources...

...2018). 4. Selection marker while engineering viruses Acr genes can be used as positive selectable markers...

... positive selection marker such as an antibiotic resistance gene is also included to select for successfully...