We narrowed to 814 results for: org

...Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski,...antigen. J Gen Virol., 73 (Pt 3), 653–660. https://doi.org/10.1099/0022-1317-73-3-653. PMID 1372038. Randall.... J Gen Virol., 68 (Pt 11), 2769–2780. https://doi.org/10.1099/0022-1317-68-11-2769. PMID 2445904.   ...protein degraders. Nat Commun., 15(1), 8885. https://doi.org/10.1038/s41467-024-52871-9. PMID 39406745. Optimizing...Caenorhabditis elegans. Genetics, 228(2), iyae126. https://doi.org/10.1093/genetics/iyae126.   Gene disruption in...abscessus. J Bacteriol., 206(3), e0033523. https://doi.org/10.1128/jb.00335-23.  Bioluminescent reporting...Systems. ACS Sensors, 9(7), 3489–3495. https://doi.org/10.1021/acssensors.3c02664. ...

...asks for strong organizational skills. You might think, “I’d be good at that! I organized my lab data so...important part of the application. Even within an organization, there can be variance. At Addgene, our content...explain why you are interested in working for that organization or group. This is particularly important for...want to talk about! You could describe your organizational system, your rationale for designing it the...title of the position] at [name of the company/organization]. I am a [adjective that describes you] scientist...that particular job, your commitment to the organization's mission, or your deep interest in the lab's...

...efforts and information provided by external organizations like the Human Cell Differentiation Molecules...research applications. eLife, 12, RP91645. https://doi.org/10.7554/eLife.91645 Biddle, M. S., Alende, C., ...flow cytometry. F1000Research, 13, 817. https://doi.org/10.12688/f1000research.154034.1 Laflamme, C., McKeever...disease gene C9ORF72. eLife, 8, e48363. https://doi.org/10.7554/eLife.48363 Uhlen, M., Bandrowski, A., ...antibodies. Nature Methods, 13(10), 823–827. https://doi.org/10.1038/nmeth.3995 Virk, H. S., Biddle, M. S., ...Journal of Pharmacology, 178(15), 2948–2962. https://doi.org/10.1111/bph.15467 Virk, H. S., Rekas, M. Z., Biddle...TRPA1. Scientific Reports, 9(1), 18500. https://doi.org/10.1038/s41598-019-55133-7 More resources from ...

...detection and identification using FnCas9. https://doi.org/10.1101/2020.04.07.028167 Broughton JP, Deng X,...detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Kellner MJ, Koob JG, ...nucleases. Nature Protocols 14:2986–3012 . https://doi.org/10.1038/s41596-019-0210-2 Lucia, C., Federico, ...-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch, J. N., Valois,...Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang, F., Abudayyeh,...COVID-19 using CRISPR diagnostics. https://www.broadinstitute.org/files/publications/special/COVID-19%20detection...available for COVID-19 research Resources on Addgene.org Get the basics about CRISPR in our CRISPR guide...

...samples. bioRxiv 2024.01.21.576499; doi: https://doi.org/10.1101/2024.01.21.576499.    LEA proteins protect...freezing. bioRxiv 2024.02.06.579238. doi: https://doi.org/10.1101/2024.02.06.579238.    Engineered and evolved...Biotechnology, 10.1038/s41587-023-02078-y. doi: https://doi.org/10.1038/s41587-023-02078-y.  Anzalone, A. V., Gao...Nature Biotechnology, 40(5), 731–740. doi: https://doi.org/10.1038/s41587-021-01133-w Pandey, S., Gao, X. ...Engineering, 10.1038/s41551-024-01227-1. doi:  https://doi.org/10.1038/s41551-024-01227-1   CHARM: A compact ...editor. Science, 384(6703), ado7082. doi: https://doi.org/10.1126/science.ado7082.  Nuñez, J. K., Chen, J...editing. Cell, 184(9), 2503–2519.e17. doi: https://doi.org/10.1016/j.cell.2021.03.025.    Recombinant antibodies...

...Popular Model Organisms by Alyssa Cecchetelli and Lukas Morgan There are many model organisms people are...interview including defining your research vision, organizing your vision into a chalk talk, and tips for practicing.... In this blog post, we’ve covered five model organisms: mouse, fruit fly, yeast, zebrafish, and worm.... by leaving a comment or emailing us at blog@addgene.org.  Thank you again for a fantastic year and happy...

...to me… The problem is that scientists sometimes forget how to communicate about their work with non-academic... is essential to stay in touch with the living organism I study, to be able to see it all scales. Most... a way to better understand and appreciate the organism, for both scientist and non-scientist alike. “...inner structures of delicate plants and other organisms without destroying the original, which could provide...Do you look for the beauty in the microscopic organisms on your lab bench? Let us know in the comments...Experimenting in an Open Source Lab Resources on Addgene.org Try the Plasmid Land Game ...

...ACS Chemical Biology, 7(11), 1848–1857. https://doi.org/10.1021/cb3002478 Kamada, Y., Tateishi, H., Nakayamada...of Misfolded CFTR. Cells, 12(23), 2741. https://doi.org/10.3390/cells12232741  Kamio, T., Kono, Y., Hirosuna...Gastric Cancer. Cancer Medicine, 14(8). https://doi.org/10.1002/cam4.70793 Laissue, P. P., Alghamdi, R....imaging. Nature Methods, 14(7), 657–661. https://doi.org/10.1038/nmeth.4344  Peach, C. J., Kilpatrick, L...Journal of Pharmacology, 178(12), 2393–2411. https://doi.org/10.1111/bph.15426 Promega Corporation (2025). Illuminating...and in vitro. Oncology Reports, 50(5). https://doi.org/10.3892/or.2023.8631 Tsien R. Y. (1998). The green...Annual Review of Biochemistry, 67, 509–544. https://doi.org/10.1146/annurev.biochem.67.1.509  More resources...

...analyses using ELISA. Peptides 72:4–15 . https://doi.org/10.1016/j.peptides.2015.04.012 Burnette WN (1981... Analytical Biochemistry 112:195–203 . https://doi.org/10.1016/0003-2697(81)90281-5 Pillai-Kastoori L,...for the user. J Biol Chem 295:926–939 . https://doi.org/10.1074/jbc.ra119.010472 Renart J, Reiser J, Stark...National Academy of Sciences 76:3116–3120 . https://doi.org/10.1073/pnas.76.7.3116 Towbin H, Staehelin T, Gordon...National Academy of Sciences 76:4350–4354 . https://doi.org/10.1073/pnas.76.9.4350 Yang P-C, Mahmood T (2012...trouble shooting. North Am J Med Sci 4:429 . https://doi.org/10.4103/1947-2714.100998 Additional resources on...articles related to antibodies Resources on Addgene.org Find written and video protocols Visit Addgene's...

...here yet? A quick look at your order status on addgene.org identifies the hold-up. Your Material Transfer...possible Tell them about the Golden Stopwatch! Any organization that deposits with and/or requests materials...to Addgene’s Tech Transfer Team: techtransfer@addgene.org Congratulations to the Golden Stopwatch awardees...to all of the winners so far! Don’t see your organization below? Don’t worry--we check periodically for...transfer offices. Drop us a line at techtransfer@addgene.org. Albert Einstein College of Medicine  Baylor...of commercialization Additional resources on Addgene.org Addgene Help Center: Tech Transfer Technology...

...Mutagenic Potential. PLoS Genet 5:e1000683 . https://doi.org/10.1371/journal.pgen.1000683 Bétermier M, Bertrand...Error-Prone Process? PLoS Genet 10:e1004086 . https://doi.org/10.1371/journal.pgen.1004086 Ghezraoui H, Piganeau...End-Joining. Molecular Cell 55:829–842 . https://doi.org/10.1016/j.molcel.2014.08.002 Ran FA, Hsu PD, Lin...Editing Specificity. Cell 154:1380–1389 . https://doi.org/10.1016/j.cell.2013.08.021 Waters CA, Strande NT...nonhomologous end joining. Nat Commun 5: . https://doi.org/10.1038/ncomms5286 Yan M-Y, Li S-S, Ding X-Y, Guo...Mycobacterium tuberculosis. mBio 11: . https://doi.org/10.1128/mbio.02364-19 More CRISPR Blog Posts from...

...Cell Explorer at allencell.org and the Allen Institute website at alleninstitute.org. A classic challenge...Cell Explorer at allencell.org and the Allen Institute website at alleninstitute.org. References 1. Roberts.../Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional Resources... Our Fluorescent Protein Posts Resources on Addgene.org Browse CRISPR Tagging Plasmids Browse the Fluorescent...

...donor DNA. Nature, 576(7785), 149–157. https://doi.org/10.1038/s41586-019-1711-4 Chen, P. J., Hussmann...outcomes. Cell, 184(22), 5635-5652.e29. https://doi.org/10.1016/j.cell.2021.09.018 Nelson, J. W., Randolph... Nature Biotechnology, 40(3), 402–410. https://doi.org/10.1038/s41587-021-01039-7 Nishimasu, H., Ran, ...and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Yan, J., Oyler-Castrillo...RNA-binding protein. Nature, 628(8008), 639–647. https://doi.org/10.1038/s41586-024-07259-6 Additional Resources... Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by...

...modulating SARS-CoV-2 infection. bioRxiv. https://doi.org/10.1101/2021.04.22.440848 Crawford KHD, Eguia R...Neutralization Assays. Viruses 12:513. https://doi.org/10.3390/v12050513 Hsieh C-L, Goldsmith JA, Schaub...SARS-CoV-2 spikes. Science 369:1501–1505. https://doi.org/10.1126/science.abd0826 Tong P, Gautam A, Windsor... of evolving SARS-CoV-2 spike bioRxiv. https://doi.org/10.1101/2021.03.10.434840 Tsang J, LaManna CM (...Detection Tools. The CRISPR Journal 3:142–145. https://doi.org/10.1089/crispr.2020.0030 Additional resources on...Browse all COVID-19 blog posts Resources on Addgene.org Find COVID-19 resources on our COVID-19 and ...

... https://wiki.hackerspaces.org/Hackerspaces OpenWorm Project Website: http://www.openworm.org/ Cat Microbiome...1,000 active makerspaces around the world. Hackerspaces.org maintains a list of active spaces near you...While C. elegans are often thought of as simple organisms , they have many levels of complexity, especially...collection of samples and sequencing is being organized and funded by someone else (KittyBiome has been...University at NASA Ames, and an instructor at Biocurious.org, Derek is bringing cutting edge technologies...

...result of the content. Here are some of my tips to organizing and running a memorable panel discussion. Check...make it look effortless to the audience, but the organizers and moderator should have a well-planned approach...panelists to the moderator. The moderator and organizers should develop a list of 4-6 pre-event interview...interview questions. The moderator (possibly with an organizer) should set up a 30 minute call with each panelist...example, at a recent event on nonprofit science organizations, I asked audience members to stand and describe...networking time. Both the moderator and program organizers can help make sure the event stays on schedule...

...e-mail us at  deposit@addgene.org Special cases Please contact us at deposit@addgene.org or +1 (617)-ADDGENE...plasmids, please let us know by contacting deposit@addgene.org. These deposits are considered large deposits.... Email the completed spreadsheet to deposit@addgene.org along with your GenBank files, plasmid sequences...library with Addgene?" or contact us at deposit@addgene.org or +1 (617)-ADDGENE (233-4363) with additional...to your strain. Please contact us at deposit@addgene.org or +1 (617)-ADDGENE (233-4363) if you’d like ...after submission, please email us at deposit@addgene.org. Include your deposit number and which data needs...

... spatial genome organization deciphering. Molecular Cytogenetics, 11. https://doi.org/10.1186/s13039-018... 2. Determining the spatial and hierarchical organization of chromatin interaction networks  A second ...Biotinylated dCas9. Cell 170:1028–1043.e19 . https://doi.org/10.1016/j.cell.2017.08.003 Liu, X., Chen, Y., Zhang...biotinylated dCas9. Genome Biology, 21. https://doi.org/10.1186/s13059-020-01973-w Additional Resources... Favorite CREs Here Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic...

...Molecular Biology. Humana Press, pp 133–148. https://doi.org/10.1007/978-1-62703-293-3_10 Walter J et al. (2020...SARS-CoV-2 receptor-binding domain. BioRxiv.  https://doi.org/10.1101/2020.04.16.045419.  Wrapp D, De Vlieger...Camelid Antibodies. Cell 181:1004–1015.e15 . https://doi.org/10.1016/j.cell.2020.04.031 Zimmermann I, Egloff...proteins. Nature Protocols 15:1707–1741. https://doi.org/10.1038/s41596-020-0304-x Zimmermann I, Egloff ...trapping of membrane proteins. eLife 7: . https://doi.org/10.7554/elife.34317 Additional resources on the...101: Secondary Nanobody Toolbox Resources on Addgene.org View the Antibody plasmid collection Browse ...

...types, spanning both prokaryotic and eukaryotic organisms. Typically, what system is used is dictated by...Titering Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols Viral Plasmids and ...cells. The FASEB Journal, 16(8), 869–871. https://doi.org/10.1096/fj.01-0995fje. Tan, E., Chin, C. S. H.,...in Bioengineering and Biotechnology, 9. https://doi.org/10.3389/fbioe.2021.796991. Urabe, M., Ding, C.,.... Human Gene Therapy, 13(16), 1935–43. https://doi.org/10.1089/10430340260355347. Vaughn, J. L., Goodwin... noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077....

...1513. doi: https://doi.org/10.1038/s41477-024-01786-w. (bioRxiv preprint: https://doi.org/10.1101/2024.02.11.579803...cargo. bioRxiv 2024.12.20.629623; doi: https://doi.org/10.1101/2024.12.20.629623.  A fast and responsive...events. Neuron, 112(22), 3680–3696.e8. doi: https://doi.org/10.1016/j.neuron.2024.08.019.  ONE-GO biosensors...platform. Cell, 187(6), 1527–1546.e25. doi: https://doi.org/10.1016/j.cell.2024.01.028. New recombinant antibodies...Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...

...proto-oncogene product. Mol Cell Biol 5:3610–3616. https://doi.org/10.1128/mcb.5.12.3610-3616.1985 Munro S, Pelham...Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002/j.1460-2075.1984.tb02263.x  Munro S, Pelham...chain binding protein. Cell 46:291–300. https://doi.org/10.1016/0092-8674(86)90746-4 Munro S, Pelham HRB... luminal ER proteins. Cell 48:899–907. https://doi.org/10.1016/0092-8674(87)90086-9  Schüchner S, Behm...neighboring sequence context. Sci Signal 13. https://doi.org/10.1126/scisignal.aax9730  Additional resources...Plasmid-based Recombinant Antibody Resources on Addgene.org  Find recombinant anti-c-Myc [9E10] at Addgene...

...variety of model organisms. Use the links below to find Gateway plasmids for your organisms of interest: ... will depend on a number of factors, like your organism, desired expression level, and experimental purpose... Genomic DNA and is available for all kind of organisms from mammals to E. coli. Glossary of Gateway cloning.... A Genetic Switch: Phage (Lambda) and Higher Organisms (Cambridge, MA: Cell Press). Additional Resources... to Quickly Verify Your Clones Resources on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic...

...but, ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum ...trolls. 3. The AMA process was simple and well organized. You can check out the r/Science AMA Submission...only small downside to the AMA from my end as an organizer/promoter was that I didn’t have the direct link... science talk here). Finally, my main content organizer is the amazing app Evernote. I have a paid account...which gives me all the features I need to save, organize and easily find the resources that I come across...do without it. So get yourself a good content organizer and build your own professional development etc...

...handbooks/Springer protocols (pp. 245–260). https://doi.org/10.1007/978-1-0716-4358-7_20  Karmakar, S., Panda...Biotechnology Journal, 22(10), 2950–2953. https://doi.org/10.1111/pbi.14416  Maher, M. F., Nasti, R. A.,...meristems. Nature Biotechnology, 38(1), 84–89. https://doi.org/10.1038/s41587-019-0337-2  Molla, K. A., Sretenovic...editors. Nature Plants, 7(9), 1166–1187. https://doi.org/10.1038/s41477-021-00991-1  Qiao, J., Zang, Y....virus-based delivery in wheat. Nature Plants. https://doi.org/10.1038/s41477-025-02023-8    Additional Resources... in Popular Culture Additional Resources on addgene.org Genome Engineering Guide Plant Plasmids and ...

...Analytical Chemistry, 83(12), 4327–4341. https://doi.org/10.1021/ac2010857  Sanger, F., Nicklen, S., & Coulson...Academy of Sciences, 74(12), 5463–5467. https://doi.org/10.1073/pnas.74.12.5463  Shendure, J., & Ji, H....Nature Biotechnology, 26(10), 1135–1145. https://doi.org/10.1038/nbt1486  Slatko, B. E., Gardner, A. F.,...Protocols in Molecular Biology, 122(1). https://doi.org/10.1002/cpmb.59  Wang, Y., Zhao, Y., Bollas, A....Nature Biotechnology, 39(11), 1348–1365. https://doi.org/10.1038/s41587-021-01108-x Additional Resources...Plasmid Quality Control Additional Resources on addgene.org Molecular Biology Reference Sequence Analyzer...

...an essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants...enzymes catalyze the transfer of carboxyl groups to organic acids to produce important cellular metabolites...post-translational protein modification. Most organisms have less than five enzymes in the entire proteome...for biotinylating only one lysine in the entire organism on the biotin carboxyl carrier protein (BCCP)....is unaffected by changes in pH, temperature or organic solvents. Uses of biotin in molecular biology Biotin...PubMed PMID: 10470036. 3. Said, H. M. “Biotin: The forgotten vitamin.” American Journal of Clinical Nutrition...

...intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based technique, validation...you are confident, especially if you work in an organism that is underrepresented in the biomedical research...Biology. Springer New York, pp 299–311. https://doi.org/10.1007/978-1-4939-8935-5_26 Stuurman N. (2012)...Fluorescence Microscopy. iBiology. https://www.ibiology.org/talks/fluorescence-microscopy/   Uhlen M, ...of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995 Additional Resources on the ...The Basics of Western Blotting Resources on Addgene.org Find plasmids encoding recombinant antibodies...

...through your organization in this field. Please also enter the billing address for your organization. We will...customer service line or sent an email to help@addgene.org, you’ve likely talked to one of us! Today we’...you time. Anyone affiliated with the recipient organization can create an Addgene account and place an order... entered during checkout. At this time, your organization will have net 30 days to complete payment for...

...conference here. The Future of Research conference is organized by postdocs for postdocs. I am delighted to see...importance of the topics to be discussed, the organizers are building valuable skills and meeting new ...Students also partner with independent career organizations to get the grants and programs they need such...offices in the last few years. However, some organizations are woefully behind and some just don't have...Symposium on the Future of Science: A postdoc-organized symposium on the future of the scientific endeavor...