We narrowed to 825 results for: org

... transposons are delivered to cells or a model organism, they insert into the host’s genome. Then, the...Eukaryotic Genomes. Annu Rev Genet 41:331–368. https://doi.org/10.1146/annurev.genet.40.110405.090448 Kumar A ...out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research.21018.1 Munoz-Lopez M, Garcia-Perez...Applications in Genomics. CG 11:115–128. https://doi.org/10.2174/138920210790886871 Pray, L. (2008) Transposons... for Genome Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084 Additional resources on the...

... Volunteering at a Professional Organization Resources on Addgene.org Check out Career's at Addgene ...contribute to the Management Team that steers the organization. As an Addgene vet familiar with the expertise...like-minded group of co-workers who want to make our organization as environmentally friendly as possible. We ...make Addgene the most environmentally friendly organization on the planet, in which case whoa! Mission accomplished...

...untagged counterparts. These findings motivated Georgieva et al. to develop new tags with defined 3D structures...aggregates. Using budding yeast as a model system, Georgieva et al. examined the effects of GFP fusions to .... To prove that inntags are indeed innocuous, Georgieva et al. created tagged versions of the cyclin Cdc28...epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small self-structured...

...planning meetings, and finding the best way to organize your time. At a conference, you could be honing...find yourself asking, "Why doesn't this product/organization/position exist yet?", you are in the early stages... about his "follow your bliss' mantra and never forgot it. Usually when I see inspiring artists who are... our transferable skills guide Resources on Addgene.org Find job listings at Addgene Get more career...

.... Nature Immunology, 25(1), Article 1. https://doi.org/10.1038/s41590-023-01689-6 LaFleur, M.W., Nguyen...immunity. Nature Immunology 20, 1335–1347. https://doi.org/10.1038/s41590-019-0480-4 LaFleur, M. W., Nguyen...system. Nature Communications, 10(1), 1668. https://doi.org/10.1038/s41467-019-09656-2 More resources on the...Genome Editing CRISPR Cheat Sheat Resources from Addgene.org CRISPR Guide CRISPR: Protocol for Genome Deletions...

...Norepinephrine. Neuron, 102(4), 745-761.e8. https://doi.org/10.1016/j.neuron.2019.02.037 Kagiampaki, Z., Rohner...vivo. Nature Methods, 20(9), Article 9. https://doi.org/10.1038/s41592-023-01959-z Mirdita, M., Schütze...all. Nature Methods, 19(6), Article 6. https://doi.org/10.1038/s41592-022-01488-1 More resources on the...Skinny on In Vivo Imaging Tools Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids...

... the organization of the nucleus in space and time, and to understanding how nuclear organization varies...varies across the life of a cell, or how organization may be altered in disease states. dCas9 gets a colorful...work to increase our understanding of chromatin organization. This fluorescent CRISPR/Cas9-based method is...

...for Gene Therapy. BioDrugs 31:317–334 . https://doi.org/10.1007/s40259-017-0234-5  Büning H, Srivastava...Methods & Clinical Development 12:248–265 . https://doi.org/10.1016/j.omtm.2019.01.008  Maurer AC, Weitzman...Transduction. Human Gene Therapy 31:499–511 . https://doi.org/10.1089/hum.2020.069  Additional resources on ...parts of the AAV transfer plasmid  Resources on Addgene.org Learn about AAV Find AAVs for your experiment...

...brain. One way to address this is by working with organisms with light accessible brains, such as zebrafish...Pharmacology and Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-010814-124803 Vlasov, ...In Methods in Enzymology (pp. 181–196). https://doi.org/10.1016/bs.mie.2018.01.022 Additional resources... precise targeted manipulation Resources on Addgene.org Browse all viral vectors available at Addgene...

...https://doi.org/10.1038/s41593-022-01094-6. (This work was also shared as a preprint: https://doi.org/10.1101...microscopy. Cell, 18, 3408 - 3425.e29. (2022). https://doi.org/10.1016/j.cell.2022.07.013.   Novel class of...Cellular Biology, 5(12), 3610–3616. doi: https://doi.org/10.1128/mcb.5.12.3610-3616.1985.       ...

...interaction, thereby changing the FP’s structural organization and activating the fluorescent signal. Blue ...extracellular molecule? By fusing a biosensor to an organelle-specific targeting signal or a transmembrane domain...pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end of the biosensor: which fluorescent...CRISPRainbow for Genome Visualization Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids...

... Future Outlook. Front Immunol 8:1589. https://doi.org/10.3389/fimmu.2017.01589 Jin B, Odongo S, Radwanska...and Therapeutics. Int J Mol Sci 24:5994. https://doi.org/10.3390/ijms24065994 Xu J, Xu K, Jung S, et al ...SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586-021-03676-z  More resources on the...Against Membrane Proteins More resources from addgene.org  Plasmids encoding nanobodies at Addgene Sybody...

... 5’ of the gene they control. Promoters can be organism or tissue type-specific, which can help you restrict...Neuronal Manipulation. Front Neuroanat 13: . https://doi.org/10.3389/fnana.2019.00093 Wilmott P, Lisowski L,...Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb.2019.276 Additional resources on the...cell-type expression in retinal cells Resources on Addgene.org Find AAVs for your experiment Browse our AAV...

...Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Maynard LH, Smith O, Tilmans...Human Gene Therapy Methods 30:195–205 . https://doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha M, Shelke...Preparations. Molecular Therapy 25:1375–1386 . https://doi.org/10.1016/j.ymthe.2017.04.001 Additional resources...viral vectors protocols and tips Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's...

...process by which cytoplasmic material, including organelles, are targeted to lysosomes for degradation. Autophagy...provides limited information about what materials or organelles are being targeted for autophagy. The development...for reporting vacuolar turnover of cytosol and organelles in yeast." Autophagy 4.2 (2008): 205-213. PubMed... sheet fluorescence microscopy Resources on Addgene.org Fluorescent protein plasmids and resources Fluorescent...

...Staining. J Histochem Cytochem 62:197–204. https://doi.org/10.1369/0022155413511620  Lu QL, Partridge TA (...Sections. J Histochem Cytochem 46:977–983. https://doi.org/10.1177/002215549804600813  Tuson JR, Pascoe EW...Cytochem : Off J Histochem Soc 38:923–926. https://doi.org/10.1177/38.7.2355173  Additional resources on the...Single-Chain Variable Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene   ...

...convalescent plasma. Nature 593:142–146. https://doi.org/10.1038/s41586-021-03471-w Garcia-Beltran WF, Lam...severity and survival. Cell 184:476-488.e11. https://doi.org/10.1016/j.cell.2020.12.015 Garcia-Beltran WF, Lam...humoral immunity. Cell 184:2372-2383.e9. https://doi.org/10.1016/j.cell.2021.03.013 Additional resources... Learn more about pseudotyping Resources on Addgene.org Find plasmids in Addgene's COVID-19 collection...

...antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a Bradbury AM, Plückthun A (2015)...recombinants once. Nature 520:295–295 . https://doi.org/10.1038/520295b Bradbury ARM, Trinklein ND, Thie...functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080/19420862.2018.1445456 Additional resources...recombinant monoclonal antibodies Resources on Addgene.org Find recombinant antibody plasmids at Addgene...

...the blog and pages on the Addgene website. blog.addgene.org The blog provides tips for working with viruses...please email us and we’ll get you writing!     www.addgene.org On the Addgene website you’ll find our updated... we distribute, please send an email to help@addgene.org. Your questions help us expand and improve our...Expression in Mammals Additional Resources on Addgene.org Overview of Viral Production at Addgene Find...

...Everyone workshop at TAGC with the three other organizers. We asked them about their experiences as they...Reproducibility for Everyone Workshop, Abitua and the other organizers found several avenues to keep the audience engaged...conference was over. Depending on the conference, organizers may make the poster available after the conference...actively reach out. It’s more proactive versus organic networking,” she says.Depending on the meeting,...

...reporter challenge, and code.org has coding tutorials for all ages. Organize your data and prepare publications...publications or your dissertation Organize data that you already collected into figures for papers or...begin working on these drafts and for a few model organisms, Micropublication will accept negative data or...

...also another free citation manager that lets you organize and cite papers. With the Zotero Connector, papers...managers. If you want more tips on finding and organizing papers, check out this related blog post: Early...Resources You Can Grad School podcast episode 2 "Organizing Literature" : I found out about Zotero here Dear...manuscripts: literature searching, reading, and organizing Tips for writing a scientific review article   ...

... number of CRISPR plasmids for that particular organism available at Addgene. Percentages represent the...used to deliver CRISPR components to cells or organisms under study. In the past we’ve left the arduous...Please send any questions or comments to help@addgene.org. Finally we’d like to thank all those who are...Viral Transductions Additional Resources on Addgene.org Visit the Viral Service Page Check Out Our CRISPR...

...me with my research in terms of preparedness, organization, and sharing data. Here are five FREE science...Archives will help both you and your lab stay organized allowing you to upload anything from protocols...came across the Gibco Cell Culture app. Gibco organizes its app into four categories: tools, analytics...Develops Lab Management Software Resources on Addgene.org Find Protoctols for Your Research Catch Up On...

... can function to break membranes of different organisms. And finally, we demonstrated that R bodies can...speculatively, R bodies might endow divergent organisms with new functions. For inspiration, we can look...Kanabrocki, J. A., Quackenbush, R. L. & Pond, F. R. Organization and expression of genetic determinants for synthesis...Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids...

...detract from the process being depicted, we can’t forget that those other components are still there in ... 2018 Chromosome Architecture and Chromosome Organization Keystone Symposium. As it happens, there are...Keystone Chromosome Architecture and Chromosome Organization. These aren’t the only investigators in the ...In-exhaustive Chromatin-related genes with plasmids on Addgene.org AGO1 BRCA1 CREBBP CUL1 H2AFX H2AFY H3F3A ...

...18(2), 67–83. https://doi.org/10.1038/s41579-019-0299-x Resources on Addgene.org CRISPR software and...information on Class 2 Cas enzymes. The information is organized in wiki format and includes biochemical properties...Biochemistry, Biophysics, and Structural Biology. https://doi.org/10.32942/X2C31F     Makarova, K. S., Wolf, Y. I...

...mutagenizing a population (typically cells or small organisms) and then assaying for a functional or phenotypical... events. To put it another way, only cells or organisms with the desired phenotype will survive and be...Science, 343(6166): 80-84 (2014). doi: https://doi.org/10.1126/science.1246981. Leemhuis, H., Kelly, R.M.... IUBMB Life, 61: 222-228 (2009). doi: https://doi.org/10.1002/iub.165.  Additional resources on the Addgene...

.... 2021. https://doi.org/10.1111/gtc.12893, Zhao et al. Nat Comm. 2019. https://doi.org/10.1038/s41467-...Santilli, R.T., et al. Sci Rep (2021). https://doi.org/10.1038/s41598-021-96051-x Engineered miniature...Xiaoshu X, et al. Molecular Cell. 2021. https://doi.org/10.1016/j.molcel.2021.08.008. ...

... be found on its website (biobuilder.org) or by emailing info@biobuilder.org. Of particular interest this...found through experiential learning. “Students may forget the technical details they hear in their classes...All Synthetic Biology Blog Posts Resources on Addgene.org Guide to SynBio Assembly Standards Find Plasmids...

... work with Drosophila as an experimental model organism. I then described the Gal4/UAS system used by ... survival. For those interested in development, organs of interest, such as the intestines, can be dissected...have!) (4). The power of Drosophila as a model organism is in its amenability to genetic manipulation....for Site-Specific Recombination Resources at Addgene.org Browse Drosophila Plasmids ...

...your current organization but also important to build relationships outside your organization. The mentor...what makes a good mentoring program. If your organization has a mentoring program, consider tapping into...upcoming mentoring post in which I describe how to organize a successful peer mentoring circle. Asking someone...

...integrins (cell-adhesion proteins) targeting different organs. This means that exosomes not only promote microenvironment...microenvironment’s malignancy but also incite different organs to become malignant. Exosomes also contain miRNA..., et al. "Tumour exosome integrins determine organotropic metastasis." Nature 527.7578 (2015): 329. PubMed...about the tumor microenvironment Resources on Addgene.org Visit our cancer research plasmids and resources...

...Antibody Fragments. Antibodies 8(2):28. https://doi.org/10.3390/antib8020028    Wessel GM, McClay DR (1986...Cytochem : Off J Histochem Soc 34(6):703–706. https://doi.org/10.1177/34.6.3084626  Zhang Q, Miyamoto A, Watanabe...microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016/j.crmeth.2022.100301  Additional resources... the Mouse on Mouse Mess in IHC Resources on Addgene.org  Browse ready-to-use antibodies at Addgene...

...can take to verify your own plasmids. Staying organized With hundreds of samples coming through our doors...first step to ensuring plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid...selection markers. You don’t need a fancy database to organize your own plasmid collection, although you will...found here. Finally, you will want to have a well-organized database of expected sequences, either for the...

...guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses...and for registration please go to http://futureofresearch.org/boston/. The Future of Research Symposium...at the Andrew Young School of Policy Studies at Georgia State University, is an expert on the academic ...experience from the perspective of both grassroots organisations that represent postdocs and institutes that ...

... time to read them. Organizations: Accounts for nonprofits, research organizations, and universities often...hashtags to library entries, which can help you organize and search for papers later. Pro tip Learn more...search terms will show up in your feed. Feeds are organized by papers published each day, week, or month. ...

...functional correction of the gene in clonally expanded organoids. This work, and many others, pushed the field ... post was originally written in 2014 by Kendall Morgan and updated in 2023 by Susanna Stroik.  References...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...1563. doi: 10.3390/ijms24021563 Resources on Addgene.org CRISPR Plasmids and Resources CRISPR 101 eBook...

...concentrations and transients in living cells and organisms. Unlike chemical indicators, these DNA constructs...constructs can be delivered to intact tissues and organs noninvasively and target a defined population of cells...Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep...protocols. 2006 Aug;1(3):1057-65. Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: ...

...lac operon assembly deposited by NEB – and don’t forget to ask DAD for some help! References Potapov,...to DNA Assembly. ACS Synthetic Biology. https://doi.org/10.1021/acssynbio.8b00333 Pryor, J. M., Potapov...ACS Synthetic Biology, 11(6), 2036–2042. https://doi.org/10.1021/acssynbio.1c00525 Pryor, J. M., Potapov...assembly design. PLOS ONE, 15(9), e0238592. https://doi.org/10.1371/journal.pone.0238592    ...

... 27649177. PubMed Central PMCID: PMC5038762. 3. George Abraham, Bobin., et al. "Fluorescent protein based...physical stresses or even apply forces (Gayrard and Borghi 2016) to a selected protein. Overcoming current...PubMed PMID: 27475975. 6. Gayrard, Charlène, and Borghi, Nicolas. "FRET-based molecular tension microscopy...FRET Based Biosensors Additional Resources on Addgene.org Find FRET Pairs Check Out Fluorescent Protein...

...or myc-tag fusions. protocols.io, doi: https://dx.doi.org/10.17504/protocols.io.261ged7xov47/v2.    Rabbit...from Morano et al. 2026. Addgene Report https://doi.org/10.57733/addgene.6tylmq reproduced under CC BY ...norepinephrine. Nature Methods, 23(3), 636–652. https://doi.org/10.1038/s41592-026-03006-z.   TCRAFT: A new approach...vitiligo. Immunity, 59(2), 477-493.e9. https://doi.org/10.1016/j.immuni.2026.01.001.    That's all for...

...imaging. Nature Communications, 16(1), 3241. https://doi.org/10.1038/s41467-025-58223-5.   TnpB, so hot right...Arabidopsis. Nature Plants, 11(5), 967–976. https://doi.org/10.1038/s41477-025-01989-9.   Make a splash with...mutations. Cell Stem Cell, 32(4), 564–580.e6. https://doi.org/10.1016/j.stem.2025.01.012.  New AAV6 and AAV11...tracer. Nature Methods, 22(5), 1101–1111. https://doi.org/10.1038/s41592-025-02670-x.  ...

...2020. https://doi.org/10.1016/j.cell.2020.08.012  Starr et al Science. 2021. https://doi.org/10.1126/science.abf9302... Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289     Novel protein cage...pathways.   Cheah et al., bioRxiv 2021. https://doi.org/10.1101/2021.01.30.428974   The CRISPR corner...

...in 720°, i.e. they are on a membrane or in an organelle, then their effective concentration will be much...the membranes into stacked structures including organized smooth endoplasmic reticulum (OSER) whorls. 2)...distorted into dense stacked membranes termed Organized Smooth Endoplasmic Reticulum or OSER if a dimerizing...in Your Experiments Additional Resources on Addgene.org Browse our Fluorescent Protein Resources Find...

...of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a “perfect repair” like...researchers can exploit for CRISPR gene editing. In organisms where HDR is downregulated, MMEJ represents another...for Your Experiment Additional Resources on Addgene.org Learn All about CRISPR on Our CRISPR Guide Pages...

...Resources: http://openspim.org/Welcome_to_the_OpenSPIM_Wiki http://www.janelia.org/lab/keller-lab ...specimens and for in vivo imaging of transparent organisms. Advances in tissue clearing Similar to light ...3DISCO (3-dimensional imaging of solvent cleared organs) method of imaging rodent brains and spinal cords...

...a co-founder of the BioBricks Foundation, an organization working to advance biological engineering openly... technical challenge. We have a strategy that organizes how we start the work, and it begins with the ...which we know and love. Boolean is in reference to George Boole who in 1854 was thinking about logic and ... Kendall Morgan...

... Sanchez MI et al., Nat methods. 2019. https://doi.org/10.1038/s41592-019-0665-7 Optimizing fluorescent... Chertkova, AO, et al., bioRxiv. 2020. https://doi.org/10.1101/160374 Nanobodies- there's a simple method...Iwaki et al., Protein Expr Purif, 2020. https://doi.org/10.1016/j.pep.2020.105607 A new multiplexed CRISPRi... Hazelbaker et al., Sci Reports. 2020. https://doi.org/10.1038/s41598-020-57500-1 The CRISPR corner By...

... number of career paths--industry, nonprofit organization, law office, journal editor, etc.--so everyone...around the world, such as BosLab here in Boston. Organizations like this one are always looking for scientists... own independent research!). There are also organizations like Science from Scientists, which try to bring...blog posts about career readiness Resources at Addgene.org Find careers at Addgene Watch out career videos...