We narrowed to 427 results for: RAP

...fragment with FKBP rapamycin binding domain of mTor (Cas9(N)-FRB-NES) resulting in a rapamycin-inducible Cas9...Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible...Cas9 for genome editing. Without rapamycin treatment, the Cas9(N)-FRB-NES fragment is actively shuttled...to the nuclear export sequence. Treatment with rapamycin induces Cas9(N)-FRB-NES and Cas9(C)-FKBP-2xNLS...splicing   The Gideon Dreyfuss lab has developed rapid-response luciferase (firefly P. pyralis) reporter...e.g. fluorescence recovery after photobleaching [FRAP]) for studying membrane dynamics, for monitoring...

... Figure 2: A bacteriograph of Michelangelo's "The Creation of Adam." This bacteriograph was created using...Mustafa Khammash's lab at ETH Zürich. “Instead of a graphical abstract, it’s a musical abstract,” says Di Ventura...). These images are based on the concept of bacteriographs created about a decade ago. To create these...

...your writing habit into beautiful graphs! I like to look at these graphs when I need that extra push to ...to stick with my writing routine. The graph of which days of the week you most often completed your habit...flagging passive voice, overuse of adverbs, and paragraphs with many hard-to-read sentences.  Grammarly ...

...advancement of SARS-CoV-2 research because they provide rapid access to validated reagents, especially during ...nonprofit research institution, has been able to rapidly propagate this strain, making it available to scientists...provides. Repositories around the world facilitate the rapid pace of SARS-CoV-2 research by removing logistic...hurdles to continue making materials available rapidly to scientists (Nature Biotechnology 2020). The ...

...advantages over pharmacological inhibitors including rapidly depleting a protein-of-interest, increasing target...system as we can use it to evaluate the effects of rapid and selective degradation of protein targets in ...machinery through binding with cereblon, leading to rapid target degradation (Figure 1). Plasmids: We generated...tagged, the dTAG lentiviral plasmids can serve as a rapid means of comparing functionality of N- and C-terminal...

...levels? Until recently, no such tool existed for rapid, customizable protein degradation. Enter TRIM-Away...antibody targeting the protein of interest led to rapid, targeted degradation that is dependent on proteasome...targeting of mTOR producing similar results to rapamycin inhibition. To assess TRIM21 toxicity, Clift et...FIRE–Cas9), combines these approaches to enable rapid and reversible recruitment of chromatin complexes...

...mRNA. These RNA tools have been used in clinical therapy to target pathogenic proteins, as well as in the... In the same vein, genotoxic stress, such as chemotherapy treatment or ionizing radiation, can also result...responses are also a concern when it comes to RNAi therapeutic delivery. Thus, balancing the amount of siRNA...just for DNA. Untwisting RNA is important for therapeutic delivery, viruses, human disease, and much more...

...contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism, but their small packaging capacity...tested the base editors in vivo and achieved therapeutically relevant editing efficiencies in the mouse ...comparable to those currently used in human gene therapy trials. They further tested the base editors by...Niemann-Pick disease type C, directly demonstrating the therapeutic potential for the split base editors. Find the...

... only for translational research, such as gene therapy, but also basic research. To improve cell-type ... with high rates of labeling are ideal of gene therapy applications, promoters that produce sparse labeling...basic as well as translational research for gene therapy. The Roska lab hopes to design improved versions...blog Read more about using AAV for retinal gene therapy Find more about precise neuronal targeting using...

...perfectly reasonable paragraph unintelligible to a lay audience. My lay paragraph typically includes a...the best place in the world for your research to rapidly progress. Need Help Building Your Scientific Network...kindly friends and family to read my non-expert paragraph and point out words or sentences that are unclear...

...article ultimately reported the development of a rapid design and prototyping pipeline that can take pathogen...the Zika virus genome with Addgene to facilitate rapid sharing of these tools with the Zika virus research...other critical needs and to further develop our rapid prototyping and sensor assembly pipeline for future...sustainability.   References 1. Pardee, Keith, et al. "Rapid, Low-Cost Detection of Zika Virus Using Programmable...

...models for developmental processes and promising therapeutic tools. The two unique characteristics that define...cells can be used for different research and therapeutic purposes. Both types have their advantages and...of post-transplantation rejection when used therapeutically High Low (if ASCs are isolated from the patient...directly from a patient’s adult cells when used therapeutically, the chances of post-transplantation rejection...

...sensitization and might open new ways for a novel therapeutic approach against drug addiction. Anxiety tests...pro-inflammatory cytokine interleukin IL-1 beta in the dorsal raphe nucleus (DRN), a region thought to be involved in... medial prefrontal cortex Drd1 neurons produces rapid and long-lasting antidepressant effects." Nature...., Alison V. Roland, and Tracy L. Bale. "Dorsal raphe neuroinflammation promotes dramatic behavioral stress...

...One of the biggest barriers to gene therapy treatment of central nervous system (CNS) diseases is the...Transport across the Blood-Brain Barrier." Molecular Therapy 27.5 (2019): 912-921. PubMed PMID: 30819613. Hordeaux...AAV-PHP. B are limited to C57BL/6J mice." Molecular Therapy 26.3 (2018): 664-668. PubMed PMID: 29428298 PubMed...dose–response and comparison to AAV PHP. B." Gene therapy 25.5 (2018): 392. PubMed PMID: 30013186. Matsuzaki...

...toxins to facilitate efficient recombineering (Khetrapal et al., 2015). They created vectors for Lambda...sacB cassette with one of three toxins from the Khetrapal paper. We selected the three toxins so that at...control of a rhamnose-inducible promoter, though Khetrapal et al. also demonstrated that the arabinose promoter... 23360965. PubMed Central PMCID: PMC3748948.  Khetrapal, Varnica, et al. "A set of powerful negative selection...

...they circulate in the blood, rather than being geographically constrained to the brain. In cases where it...underexplored sites on the AAV capsid. Molecular Therapy - Methods & Clinical Development, 26, 331–342. ...for Robust CNS Transgene Expression. Molecular Therapy - Methods & Clinical Development, 15, 320–332. ...-PHP.B Are Limited to C57BL/6J Mice. Molecular Therapy, 26(3), 664–668. https://doi.org/10.1016/j.ymthe...Transport across the Blood-Brain Barrier. Molecular Therapy, 27(5), 912–921. https://doi.org/10.1016/j.ymthe...Non-human Primates Compared with Mice. Molecular Therapy, 27(11), 2018–2037. https://doi.org/10.1016/j.ymthe...

...are released in each synaptic event and they are rapidly taken up by postsynaptic receptors and transporters...sensitivity. iGluSnFR4f is well-suited for imaging rapid synaptic events such as in the somatosensory cortex...much to learn about their myriad functions and therapeutic potential. Our partners at the Institute for ...critical — factor for successful experiments or therapies. Using AAV vectors with enhancers that drive cell-type-specific...

...of the DA neurons produce behavioral and electroencephalography evidences of arousal in mice previously...enabled the drawing of a detailed and functional topography of the neural circuit architecture of the brain...exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological Disorders. Humana Press, New York...26135556. Hudry, Eloise, and Luk H. Vandenberghe. "Therapeutic AAV gene transfer to the nervous system: a clinical...

...software could also be used as a simple tool to rapidly find one or a few gRNAs suggested to be effective...appropriate phenotypic readout, similar mapping could be rapidly performed by targeting all potential components...identified for each gene. This file can be used to rapidly get an overview of the list of gRNAs generated....screen identifies genetic vulnerabilities and therapeutic targets in acute myeloid leukemia." Cell reports...

...Assembly Standard and New E. Coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional....2020.100135. Fonseca, João Pedro, et al. “A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian...Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors...Abby V., et al. Modular Vector Assembly Enables Rapid Assessment of Emerging CRISPR Technologies. Cold...