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We narrowed to 749 results for: SOM

Showing: 81 - 100 of 749 results

  1. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...protein. At some point during this interrogation process, they will almost invariably say something like “Oh...company is “…where molecules become real”.  We sometimes forget that the models we create are around 30... presented with a physical model of his large ribosomal subunit, he kept sliding his palm across the surface...determined electron density, have been known to say something silly like “See there, arginine 53 really is right...them to slow down and focus their attention on something tangible. Under the guidance of a trained educator...business – 3D Molecular Designs – to commercialize some of the best instructional materials that resulted...the education community that All models are wrong….Some models are useful. While a physical model of a protein...

  2. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...." At the end of the day, someone has to write the article and that someone is going to be you. So, just...word limit, formatting guidelines, etc.) you have some criteria to shape the document. Get and use a reference...short bursts (even twenty minutes was enough to make some progress). For most researchers, it’s not always...citing both because I used both. Curate and present some useful data Typically lists and pictures are the...active role in writing and editing, so I always had someone to send drafts back and forth with. That being ...This is the time to transform the document into something cohesive-- change the sentences, make it flow,...Get feedback After you’re done editing, send it to some actual experts in the field for their feedback on...

  3. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...and offering advice on areas where you might need some help. Today, we’re highlighting an oft-underlooked... for your brainstorm session is to start with something completely unrelated to the issue and consider...out-of-the-box concept and pare it down until you have something tangible and applicable to your situation.   Thinking...   Thinking Outside the Box: Team Approaches At some point or another, you’ll find yourself needing to brainstorm...opportunity to speak out and prevent groupthink? Here are some ideas that I have found helpful in my team brainstorming... We become so fixated with the idea of making something the best it can be that it seems impossible to...stuck on a problem is to step away from it. Find something unrelated to do (many Addgenies enjoy rock climbing...

  4. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...popular genomic editing systems in E. coli. However, some users pointed out that this system did not function...recently, we’ve developed a new system “Multicopy chromosomal integration using CRISPR-associated transposases... both plasmids into the cell. The edit in the chromosome can now take place. After editing, pTargetF can...great for experiments that require only single chromosomal integration (ex: genome editing), our MUCICAT...transposase system, INTEGRATE, is best for multicopy chromosomal integration (ex: for protein expression) (Zhang...Zhang et al., 2020). Multicopy chromosomal integration for gene expression is an alternative versus plasmid-based...advantages: Fast and site-specific multicopy chromosomal integration of cargo without using screening ...

  5. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...could induce seizures or some other unwanted phenomenon or it could reveal something fascinating. Behavior...in studying, but, in today’s post, we’ll discuss some of the important considerations you should think...to be fully summarized in a short blog post, but some examples I’m personally interested in include: intracranial...watch what happens. Who knows, you may observe something that could move your project onto the exciting—and... ways 1) your hypothesis is wrong or 2) there is some problem with the technical implementation of your...would be willing to teach you. An essential, yet sometimes overlooked, aspect of scientific research is that... of this; research benefits when the advances of some are shared with all. Due to the versatility and ...

  6. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...plasmid is in general, it's time to take a look at some of its parts. Elements such as the antibiotic resistance...having fewer parts than those in eukaryotic cells. Some promoters are constitutively active and on all the...boundary elements, insulators, and silencers; however, some "leakiness" of transcription may occur. This is ...created synthetic promoters, which typically include some combination of other promoter elements, and tend...have included two reference tables below listing some of the most common bacterial and mammalian promoters...May contain an enhancer region. Can be silenced in some cell types. EF1a General expression mRNA Strong...  Active in dicots, less active in monocots, with some activity in animal cells. Ubi General expression...

  7. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ...decisions. As someone who was not necessarily a fan of the sciences, I knew I would run into some challenges...to. Brandon Solis, marketing intern. Something that I struggle with as I’ve been thinking about...was helpful to have Addgenies reassure me that someone would always be there to help me. As the Marketing...cute and on-brand templates for these posts. Some of recent Meet an Addgenie posts on Instagram! ...actually make connections with people. These were some of the things that really made me feel welcomed....been given the opportunity by Addgene. I have met some of the most genuine people who have shared amazing...

  8. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...you’ve been able to check out some of the many applications of AAV in some of our other blog posts and ...AAVs) make fantastic gene delivery vehicles for episomal gene expression and are particularly useful for...editing system frequently over the past fifteen years. Some examples from their lab include using AAV to introduce...and have also been able to browse some of the ready to use AAV available through our viral service. In addition...Manu, et al. "Facile methods for generating human somatic cell gene knockouts using recombinant adeno‐associated...Jung-Sik, et al. "Intact cohesion, anaphase, and chromosome segregation in human cells harboring tumor-derived...engineering using adeno-associated virus (AAV)." Chromosomal Mutagenesis. Humana Press, New York, NY, 2015...

  9. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ... to give the audience something to think about, argue with, or learn from. Some of the best talks I have.... Two of my jobs have indirectly resulted from someone seeing me speak in a non-interview setting. There... resources on self-promotion (how hard it is for some people, especially women), visibility (how to get...comes. 5. Choose interesting content Talk about something you know a lot about and that you care about. ...company you are visiting works on them. Of course, if some of your work does relate, make sure to include that...with your confidence. It is best not to be asked something that you don't know the answer to, but if you ...speakers don’t have to work at it. You have to put in some effort to be the best speaker you can be. Seek out...

  10. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...protein domains to the appropriate locus has been cumbersome. To overcome many of the limitations intrinsic...regulation has roles in many cellular functions, from endosomal trafficking to signaling pathways to the cell ...codons from the antibiotic resistance cassette, a ribosome binding site, SacI restriction site and a linker...system utilizes the bulldozer-like quality of the ribosome during translation, which knocks off all mRNA ...mammalian cells. Multicolor CRISPR labeling chromosomal loci in genome Thoru Pederson’s lab at the University...dynamic interactions between intra- and inter-chromosomal domains. The authors anticipate that this tool..., genome rearrangements and cancer-associated chromosome shattering. This method is based on the recognition...

  11. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ... parts of CRISPR. The bacterial chromosome encodes a tracrRNA (in some systems including Cas9), Cas proteins...DNA, whereas some mismatches can be tolerated outside this region. Conclusion Ready for some more details...DNA CRISPR array: The section of the bacterial chromosome that stores a genetic record of previously encountered...by the Cas9 protein from the appropriate species. Some Cas proteins, like LbCas12a or AsCas12a, do not ...system for laboratory applications. At Addgene, we sometimes refer to the programmable guide sequence as the...

  12. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...building strong connections with coworkers and doing something meaningful with my career. I can’t pinpoint...summer travel plans while eating lunch. There was something so special about her pure interest and curiosity...biweekly report of our social media analytics, something that had never been done at Addgene before. This... social media strategy. We ended up implementing some of these changes and seeing early results that suggest... and trampoline all at once. I was able to do something that was challenging while helping us share our... at Addgene have become my friends, mentors, and some of my favorite people to talk to. This six month...confident in myself, my abilities, and my future - something I didn’t expect from a co-op experience.  Sarah...

  13. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...better with some variety in their job. Good relationships with clients and colleagues Sometimes this means...skills. Managing a team is hard and most people have some learning they need to do before they can be good...type of bad manager have you had?). You can avoid some of these common pitfalls of management and still...when making decisions, assignments and polices. Sometimes the boss has to be the boss and make difficult...because, let’s face it, we are special. Below are some special characteristics of scientists in the work...deadlines (there’s that need for flexibility, sometimes taken to an extreme by scientists) Are highly ...

  14. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...recommendations on some ways to get started sharing your science with the community. Some of them you can...ask and answer questions and hopefully discover something new. Science involves success and, more often,...not just the details of your science but also something about how science really works. 2)   Read science...your own twitter feed or talking about and sharing some science on the one you already have. Read my earlier...SciFund Challenge. While the goal is ostensibly to get some funding for your research, SciFund is really motivated...finally understood why he cared to study mice on some distant island. He got a little money for his trouble...

  15. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...2017). With the right conditions and parameters, some people find it can detect even smaller differences...copy of a transcript. But this sensitivity can sometimes cause as many issues as it solves, so understanding... could easily be its own deep dive, but here are some general helpful principles (and I recommend Bustin... multiple reference genes whenever possible (Vandesompele, 2002), and it's possible to multiplex your ...documented in the literature, a geNorm study (Vandesompele, 2002) will help you select the appropriate ...produce reliable and reproducible data. Here are some tips to help you in your execution:  Accuracy is...Passive Reference Dyes Whatever your pipetting method, some variation will occur. A passive reference dye, such...

  16. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present...present on different cell types and sometimes vary between target species or even strains. Pro tip! Getting...serotype for Addgene’s Packaged on Request service. Some AAV1 tools are available through our in-stock viral...serotype for Addgene’s Packaged on Request service. Some AAV2 tools are available through our in-stock viral...serotype for Addgene’s Packaged on Request service. Some AAV5 tools are available through our in-stock viral...serotype for Addgene’s Packaged on Request service. Some AAV8 tools are available through our in-stock viral...available through our Packaged on Request service, but some AAV9 tools are available as in-stock preparations...

  17. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...sites on different chromosomes in living cells, the system requires high-copy chromosome-specific loci in...study the spatial and temporal arrangement of chromosomes and how these arrangements may affect nuclear...based multicolor labeling system to visualize chromosomal loci in human cells (Ma et al., 2015). To do ...inter-locus distances. They tested this system out on chromosome 19 by targeting 46 repetitive loci that contained... (PR1), the p-arm (T1), and the q-arm (T2) of chromosome 19. Image from Ma et al., 2018 with permission...Hanhui et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proceedings of the National...

  18. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...support your favorite local exercise spot or find something new. We usually have yoga class twice a week at... #SocialDistancing may send a wrong message for some people. We should recommend #PhysicalDistancing....those of you who have the desire and ability to, some communities are looking for bench scientists to ...foster a pet, for example. If you are looking for some resources specific to self-care during the COVID...networking you can do online. See if a scientist or someone you’re connected with would like to do an informational...more independently. For example, they could do something like puzzling or crafts, while listening to podcasts...productive as you would be in a normal situation. For some other resources on working from home, check out ...

  19. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...Relationships need nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have a...ways to strengthen your new relationships Here are some ways to go about strengthening a new relationship...there’s good news.  Send an email if you have something to ask or say. It can be as simple as “it was ...appreciation. Send a thank you note or gift to someone who helped you succeed in any way. Don’t believe...strawberries for helping with an introduction that helped someone get a job interview. Even just a thank you email...is having a mutual acquaintance connect you to someone who you need to speak to for informational or career...ask me about the different arms of this starfish sometime, but as of yesterday, I have 4,700 LinkedIn connections...

  20. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ... allows it to replicate episomally, essentially remaining an extrachromosomal part of the host genome ...single-stranded DNA parvovirus. AAV can infect humans and some other mammals but is not currently known to cause...This is large enough for many applications, but in some cases multiple AAVs (co-infection), an alternative...humans, many mammals, and even the avian population. Some adenoviruses are known to cause respiratory disease...Adenovirus generally has high immunogenicity, but some strains have been engineered to induce a lower inflammatory...AAV’s several years). But these viruses are roomy: some adenoviruses pack up to ~35 kb of space for cargo...
Showing: 81 - 100 of 749 results