We narrowed to 363 results for: his
-
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....express and purify His-tagged Cas9 from E. coli using a plasmid such as pET-28b-Cas9-His from Alex Schier...
-
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m....member of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing...the creation of innovative technologies. To date his group has develop several popular CRISPR-based tools... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m.... extreme sports enthusiast. He recently obtained his Ph.D. in neuroscience from the University of Rochester...inflammation and neurodegeneration. He now spends his time learning and writing about science whenever ... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....University. Max Shen is a Ph.D. candidate at MIT. His research uses applied machine learning and statistical...synthesis are four examples of technologies pioneered in his laboratory. Learn more here. Additional resources...effect on plasmid requests Before we began any sophisticated analyses, we first visualized our data in various... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog PostPublishedJuly 2, 2020, 12:40 p.m....conformation capture (3C) based methods, such as 5C or Hi-C (Han et al., 2018). Think of 3C methods as taking...outperformed other 3D chromatin methods such as ChIA-PET or Hi-C, by increasing both the number of unique chromatin... -
Save Time with Transient Plant Leaf Transformations
TypeBlog PostPublishedJuly 25, 2019, 12:03 p.m....Northeastern University in Boston, MA. He obtained his BSc and MSc in Plant Biotechnology from Leibniz Universität... Universität Hannover (Germany), before starting his work in the Lee-Parsons Lab. References D'Aoust...below. The GUS gene (β-glucuronidase) with histochemical GUS-staining is a very useful visual reporter... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog PostPublishedJune 25, 2024, 1:15 p.m....editing experiments come into play. Nicolas Mathis received his PhD from Gerald Schwank's lab at the University...developed a strong passion for computational biology. His expertise includes applying machine learning techniques...efficiencies, we could train our prediction model PRIDICT (Mathis & Allam et al., 2023). We trained our models in...training led to the recent development of PRIDICT2.0 (Mathis et al., 2024). The additional datasets used in ...pegRNA editing efficiencies for specific targets (Mathis & Allam et al., 2023, 2024; Yu et al., 2023; Koeppel...variations in chromatin context. Our recent studies (Mathis et al., 2024) and concurrent research by the Shendure...Cell. https://doi.org/10.1016/j.cell.2024.03.020 Mathis, N., Allam, A., Kissling, L., Marquart, K. F., ... -
New Podcast Segment: Hot Plasmids
TypeBlog PostPublishedMay 17, 2017, 3:37 p.m....from John Chodera's Lab - Get high expression of His-tagged human kinases in bactera. Listen to Our Segment... -
Streaking for Single Colonies: The Streak Plate Challenge
TypeBlog PostPublishedMarch 2, 2021, 2:15 p.m..... So when Dave Westenberg taught this concept in his microbiology lab course, he decided to add a bit ... -
Congratulations, Deck The Lab winners!
TypeBlog PostPublishedJan. 14, 2021, 1:30 p.m....Matthijs Dorst’s Deck the Lab Nativity scene below his Christmas tree! It is that time of the year again... -
A Scientist's Guide to Ebola
TypeBlog PostPublishedOct. 16, 2014, 3:01 p.m....coverage on the subject in the New York Times and on his blog, where he has offered to answer readers' questions... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m....in Molecular Plant Sciences since December 2020. His research focuses on the efficiency of transposon-... with low efficiency. Sheng Yang obtained his Ph.D. from Shanghai Institute of Biochemistry, Chinese... -
CRISPR Activation: A Practical Guide
TypeBlog PostPublishedAug. 18, 2016, 2:30 p.m....member of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing...the creation of innovative technologies. To date his group has develop several popular CRISPR-based tools... -
MassBioEd Career Exploration Day at Addgene
TypeBlog PostPublishedNov. 13, 2015, 3:30 p.m....Scientist Jessica Welch showed some highlights of his travels from 2015, including a great European photo... -
Science Communication for Everyone
TypeBlog PostPublishedApril 16, 2019, 1:05 p.m....minorities to study STEM subjects. Here, Sadangi and his team answer a few questions about The Addictive Brain... -
Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies
TypeBlog PostPublishedFeb. 26, 2019, 1:40 p.m....school, I came across Joseph Campbell, and read about his "follow your bliss' mantra and never forgot it. Usually... -
Microbes: Look, Listen, and Tell
TypeBlog PostPublishedAug. 28, 2018, 12:04 p.m....methods for cellular imaging with ultrasound. In his research he applies naturally occurring bacterial... -
Editor's Choice, September 2016
TypeBlog PostPublishedOct. 7, 2016, 4 p.m....out our interview with Michael Koeris to learn how his company uses phage to test for Listeria in food products...surpassed 60,000 views for the month of September! Historically we do better in September than in the summer... -
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats
TypeBlog PostPublishedSept. 9, 2016, 2:43 p.m.... the Genetic Perturbation Platform at the Broad. His mom always told him that he has the best words and... -
Editor's Choice, August 2016
TypeBlog PostPublishedSept. 2, 2016, 1:32 p.m....techniques researchers are using. The detailed results of his survey can be found in the full post but TLDR: many...