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Addgene

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We narrowed to 842 results for: NTS

Showing: 571 - 580 of 842 results

  1. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...they successfully imaged multiple subcellular compartments over several minutes using Nano-lantern fusion...single molecule imaging. Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job...Addgene. Suzuki, Kazushi, et al. “Five colour variants of bright luminescent protein for real-time multicolour...

  2. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ... and you’ll need to track it throughout your experiments.  Now that that’s out of the way, you can put... reaction to understand if any of your buffer components could affect the reaction. For instance, sodium... - antibodies that are a good match for your experiments, and avoid the awkwardness of getting the right...

  3. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...idea that would evolve into Sample6 as graduate students in Dr. Jim Collin’s Lab in 2009. From 2009 to ... an idea or product? Please let us know in the comments section! And read on for more great information...contributed by Julia Phillips and Fiona Beckman Acknowledgments Thank you to Dr. Mike Koeris (Co-founder and...

  4. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...reactions on tissues can be cumbersome Tyramide reagents can be expensive Can only stain with one HRP-RANbody...consider leaving a link to its sequence in the comments below. References Sanes, J.R., & Yamagata, M....versatile, small, sensitive immunohistochemical reagents. PNAS. PubMed PMID: 29440485. Additional Resources...

  5. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...It’s also a great way to keep track of past accomplishments. Follow all the rules of good resume writing...writing. Include examples of concrete accomplishments in both the technical and “plus skills” areas. Include...’s news and updates. Share your own good ideas, events and favorite resources. Respect the network and...

  6. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    Published
    Feb. 28, 2019, 1:34 p.m.
    ...about it. Building a diverse team with different talents and expertise Fortunately, the eLife ambassadors... had previous experience organizing open access events and grassroots efforts. After thoughtful conversations...Twitter (@repro4everyone), or email the team. Acknowledgements One of the highlights of 2018 for me was to...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...such as RNaseZAP. When harvesting tissues, cells, plants, fungi, or bacteria, keep samples cold and work...the homogenate was loaded onto cesium-chloride gradients and ultracentrifuged for up to 20 hours to separate...instead of RNA separation using cesium-chloride gradients, water-saturated phenol, sodium acetate, and chloroform...

  8. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...restriction sites to easily assemble multiple DNA fragments. This system has been used to construct plasmids...McCormick lab generated knock-out and knock-in mutants, determined protein expression levels from different...critical for reducing Cre toxicity in long-term experiments.  Figure 4: The C-terminus of Cre (CreC...

  9. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ... 4B and C in Zhou et al for details on these experiments. Photoswitchable kinases could possibly be used...can I try using photoswitchable kinases in my experiments? You can find the plasmids from Zhou et al here...Carrasco, and Markus W. Covert. "High-Sensitivity Measurements of Multiple Kinase Activities in Live Single...

  10. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ... aggregate. The basics and pitfalls of these experiments are discussed here. In vitro tests for aggregation...your cell type of interest before starting your experiments.  Selecting a non-oligomerizing protein A fluorescent... Of course, you want the ‘latest and greatest’ variants with the best properties for your research. But...
Showing: 571 - 580 of 842 results