We narrowed to 855 results for: NTS

...candidate transcript targets analyzed. CRISPR-Csm presents an attractive alternative for nuclear-RNA targeting...hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you’re looking for... temperatures, and in the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods...

...Once you’ve identified some fly stocks and other reagents of interest, the next question to ask is what ...background. In other cases, YFG or one of its mutant variants will need to be overexpressed or knocked down....Although it’s often very easy to get GAL4 UAS experiments up and running with fly lines that are already...

...high-security facility needed to perform the experiments to demonstrate that this approach was useful ...diverse. Some of them are undergraduates or PhD students who are just starting their journey into science...stops these one-time favors from becoming starting points for long-lasting partnerships Join us! We are a...

...a degron, a domain that induces degradation. In plants, auxin binds to TIR1 of the SCF-TIR1 ubiquitin ...versatility of the engineered SpCas9-NG and its variants can be used to engineer specific sites that were... factors developed thus far for these latter experiments, such as transcription factors tethered to dCas9...

...cells (as demonstrated in different cellular environments such as retina or glioblastoma). They also found...classical purification protocol involving iodixanol gradients. The current purification protocols for isolating...decipher the mechanistic aspects behind these improvements. What have exosome AAVs achieved so far? Exo-AAVs...

...measured through their triglyceride or cholesterol contents (what is commonly called bad cholesterol). However... and localization in vivo. The LipoGlo system represents a new, intriguing approach to expand our understanding... and offers many advantages over traditional measurements such as patch clamp recordings. However, technical...

...relationships between microbes and insects, mammals, and plants, among others. I particularly enjoyed the emphasis... their paths cross with each other and with the events of the times."     - Aliyah Weinstein, Marketing...community know what your favorite book is in the comments! Additional resources on the Addgene blog Learn...

...Recombinases And more! Control AAV For many AAV experiments, we recommend validating the serotype in your...vectors to more serotypes. We currently package four variants of these biosensors, which are optimized for specific... should improve the specificity of influence measurements by reducing photostimulation of the axons and...

...Darrell Kotton 2. Stem cell scientists care about patients As any grad student who has spent years studying...: Dr. Yamanaka talked about his clinical trial patients with advanced macular degeneration; Laurenz Studer...focused on translational research is encouraging. Patients are key to the research, but they are also the...

...) and are ready to dive into your functional experiments! Whether you’ve cloned the plasmid yourself or...determine the relative sizes of the resulting DNA fragments. Restriction digests are commonly used to confirm...longer to achieve good separation of larger DNA fragments. I hope these tips demonstrate that plasmid ...