We narrowed to 855 results for: NTS

...they successfully imaged multiple subcellular compartments over several minutes using Nano-lantern fusion...single molecule imaging. Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job...Addgene. Suzuki, Kazushi, et al. “Five colour variants of bright luminescent protein for real-time multicolour...

...the N and C terminal segments respectively of a GFP dependent Cre. The two segments come together in the...transduction patterns empirically for their own experiments. To this end, we offer control AAV in smaller...

...being depicted, we can’t forget that those other components are still there in real life.  Certainly referring...specifically study it. They might be researching enhancer elements that control the transcription of a gene a megabase...versatile, small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A...

... and you’ll need to track it throughout your experiments.  Now that that’s out of the way, you can put... reaction to understand if any of your buffer components could affect the reaction. For instance, sodium... - antibodies that are a good match for your experiments, and avoid the awkwardness of getting the right...

...skills, while also learning about new scientific developments and helping scientists is a win-win for me. ...shared in the post. And the blog writing tips are elements I try to include in each post I write for the ...your research or career? Share your story in the comments below!   Additional resources on the Addgene...

...idea that would evolve into Sample6 as graduate students in Dr. Jim Collin’s Lab in 2009. From 2009 to ... an idea or product? Please let us know in the comments section! And read on for more great information...contributed by Julia Phillips and Fiona Beckman Acknowledgments Thank you to Dr. Mike Koeris (Co-founder and...

...wash steps removes excess proteins and other contaminants. The protein of interest is then eluted using...polyhistidine-metal ion interaction is not disrupted by denaturants (Hochuli, 1988). In some cases, proteins can ...more stringent wash conditions and remove more contaminants without losing your protein of interest. But...

... 4B and C in Zhou et al for details on these experiments. Photoswitchable kinases could possibly be used...can I try using photoswitchable kinases in my experiments? You can find the plasmids from Zhou et al here...Carrasco, and Markus W. Covert. "High-Sensitivity Measurements of Multiple Kinase Activities in Live Single...

... aggregate. The basics and pitfalls of these experiments are discussed here. In vitro tests for aggregation...your cell type of interest before starting your experiments.  Selecting a non-oligomerizing protein A fluorescent... Of course, you want the ‘latest and greatest’ variants with the best properties for your research. But...

...such as RNaseZAP. When harvesting tissues, cells, plants, fungi, or bacteria, keep samples cold and work...the homogenate was loaded onto cesium-chloride gradients and ultracentrifuged for up to 20 hours to separate...instead of RNA separation using cesium-chloride gradients, water-saturated phenol, sodium acetate, and chloroform...