We narrowed to 825 results for: org

...number of factors, including the age of the model organism, injection site, and titer. Addgene’s Viral Vector...production. Neuromethods, 195, 77–101. https://doi.org/10.1007/978-1-0716-2918-5_5 Haery, L., Deverman.... Frontiers in Neuroanatomy, 13. https://www.frontiersin.org/articles/10.3389/fnana.2019.00093 McDade,...Molecular Biology, 115, 31.5.1-31.5.13. https://doi.org/10.1002/cpmb.8...

...Saudi Arabia. Bioinformation, 14, 53–59. https://doi.org/10.6026/97320630014053 Barlow-Anacker, A., Bochkov...cell activation. PLOS ONE, 12, e0180664. https://doi.org/10.1371/journal.pone.0180664 Boix, F., Llorente... Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23 Jhunjhunwala, S., Aresta-Dasilva...Implant Materials. PloS One, 10, e0137550. https://doi.org/10.1371/journal.pone.0137550 More resources on ...

... that the drug prevents the growth of a living organism. As for multi-drug resistance: definitions vary...vary in the literature, but generally organisms with multi-drug resistance are resistant to three or more...is an intense amount of competition between microorganisms for available resources. This competition drives...Our Microbiology Pages Additional Resouces on Addgene.org Check Out Our Microbiology Pages ...

...Nonetheless, the initial release caused a storm as the organization left open the question of how much data was ...to make the underlying data easily available, organized in a form that can be understood by others, balanced...each individual video. To PLOS’s credit, the organization subsequently clarified what the policy intent...researchers. Yet accurate and comprehensible organization of data is essential for efficient science. ...

...your host organism. Because transcription machinery differs between cell types or organisms, promoters...GFP Resources on Addgene.org Find Plasmids for Your Research     ...  Kendall Morgan...

...functional, fluorescent protein in almost all organisms and cell types, (ii) no need to add a co-factor...protein are available (Cranfill et al., 2016 and FPbase.org). A number of variants remain fluorescent under...mTurquoise2, not shown) remains fluorescent in acidic organelles (Figure 1), showing that its acid tolerance is...Fluorescent Proteins 101 eBook Resources on Addgene.org Browse the Fluorescent Protein Collection Find...

...Guest blogger Todd Waldman, Professor at Georgetown University, contributed to this post. Adeno-associated...recombination pathway. The Waldman lab at the Georgetown University School of Medicine has used this gene... at the Lombardi Comprehensive Cancer Center, Georgetown School of Medicine. His research currently focuses...Made in Heaven: CRISPR and AAV Resources on Addgene.org Check out our Viral Service Find AAV Vectors...

...Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016/j.jneumeth.2008.05.003 Ghosh, R., Gilda... Review of Proteomics, 11(5), 549–560. https://doi.org/10.1586/14789450.2014.939635 Gilda, J. E., & Gomes...Analytical Biochemistry, 440(2), 186–188. https://doi.org/10.1016/j.ab.2013.05.027 Additional Resources on... and Optimizing a Western Blot Resources on Addgene.org Addgene's Western Blot Protocol  ...

...Research Volume 42, Issue 22, Page e168. https://doi.org/10.1093/nar/gku936 Eva K. Brinkman, Arne N. Kousholt...Research Volume 46, Issue 10, Page e58. https://doi.org/10.1093/nar/gky164 Cancellieri S, Canver MC, Bombieri...editing. (2019) Bioinformatics 36:2001–2008. https://doi.org/10.1093/bioinformatics/btz867 Haeussler M, Schönig...selection tool CRISPOR. (2016) Genome Biol 17: https://doi.org/10.1186/s13059-016-1012-2 Find more CRISPR Resources...

...rights (2). Less formally, governments are the organizations that make and enforce the rules of the lands...climate change, the safety of genetically modified organisms (GMOs), the absence of a link between vaccines... while their website lists over 200 partner organizations, and 514 satellite marches are scheduled to ...Chronicle of Higher Education: “March for Science” Organizer Says It’s About the Public, Not the Scientists...

...the more detailed questions the organizers wanted to touch on in organizer David Riglar’s blog post. Essentially...Symposium On the FOR website, the FOR Boston 2015 organizers indicate that the main question they wanted to...panel was that there are many companies and organizations (including Addgene), that need people who are...

...in the comments or shoot us an e-mail at blog@addgene.org and maybe we can write a blog post about it! ...including subcellular visualization of 6 different organelles, a cell cycle indicator for mouse embryonic stem...mitochondrial fusion with a new tri-chromatic assay Georg Ramm’s lab at Monash University has developed a ...viability. In their recent work in Nature Methods, Georgieva et al describe the rational production of a new...have fewer effects when fused to your POI. Georgieva et al developed the inntags via a bioinformatics...information on inntags, read our recent blog post. Georgieva, et al. Nature Methods. 2015 Aug 31. CRISPR-Display...monitoring cargo trafficking, and for discovering organelle contact sites. This suite of deposited fluorescent...

...has also been implemented in a variety of other organisms, such as sphingomonads and bacillus.  ADH1 The...mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750-6-43Kaczmarczyk A, Vorholt JA...overexpression transgenes. Sci Rep 12, 3835 (2022). https://doi.org/10.1038/s41598-022-07852-7 De Boer, Herman A., ...and PolyA Signals FLEx Vectors Resources at Addgene.org Tetracycline Collection ...

...Cell Culture. Anal Chem 84:4227–4232 . https://doi.org/10.1021/ac2033112 Ligasová A, Vydržalová M, Buriánová...Fluorescence Microscopy. Cells 8:1510 . https://doi.org/10.3390/cells8121510 Sung H, Kang SH, Bae YJ, Hong...Lines. In Vitro Cell Dev Biol Anim 38:86 . https://doi.org/10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2...for working with viral vectors Resources on Addgene.org Check out Addgene's written and video protocols...

...Psychological and Personality Science 11:647–657 . https://doi.org/10.1177/1948550619882032 Chrousos GP, Mentis A-...threatens diversity. Science 367:749.2-750 . https://doi.org/10.1126/science.aba8039 Cordova FA (2016) Embrace...Embrace uncertainty. Science 351:994–994 . https://doi.org/10.1126/science.351.6276.994 Moore E (2021) One...I built a network of mentors. Science. https://doi.org/10.1126/science.caredit.abi6939 Additional resources...

...of Scientific Instruments 43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow Cytometry...Current Protocols in Immunology 120: . https://doi.org/10.1002/cpim.40 Picot J, Guerin CL, Le Van Kim ...instrumentation. Cytotechnology 64:109–130 . https://doi.org/10.1007/s10616-011-9415-0 Additional resources ...proteins Learn more about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody...

... blood stream to commute to and invade multiple organs, and most importantly escape the immune mechanisms...environments for tumor cells spreading to other organs during metastasis. Evading Recognition Tumor cells...2002): 991-998. PubMed PMID: 12407406. 4. Weiner, George J. "Building better monoclonal antibody-based therapeutics...Ghana Modulate Cancer Signalling Resources on Addgene.org Check out Our Cancer Collection Pages Use the...

...biology world, many of scientists’ favorite model organisms belong to a group called dicots — think Arabidopsis...-Gamboa, L., Saleh, L., Pathak, S., Swyers, N., Morgan, A., Meerdink, S., Kuzio, C., Calderon, S., Sheng... Grasses. Plant Biotechnology Journal. https://doi.org/10.1111/pbi.70480 Additional Resources Browse...

...of a lawyer can bring a new perspective to an organization. I'm used to thinking about the downstream results...how to manage them without them paralyzing an organization. I think these are things that I can bring to...that business experience and judgement to the organization and see how I can help. Q: What do you enjoy...

...research with the community, so email us at blog@addgene.org if you are interested. Addgene's Most Popular...Plasmids 101: Origin of Replication By Kendall Morgan Save Time and Money by Making Your Own Competent...101: The Promoter Region – Let's Go! By Kendall Morgan Plasmids 101: Mammalian Vectors By Marcy Patrick...

...scientists to edit the genome of virtually any organisms on this planet, CRISPR-Cas systems were merely... to quickly detect the presence of infectious organisms (ex: SARS-CoV-2) and genetic mutations. By providing...Download our CRISPR cheat sheet Resources on Addgene.org Find the Cas14 plasmids here Checkout our CRISPR...

...tissue. “The brain is such an amazing and dynamic organ,” Konermann explained. “It helps us adapt to influences...use is by no means limited to particular model organisms or cell types. “This tool is really flexible and... Kendall Morgan...

.... Even more remarkably, 1,160 labs from 522 organizations in 41 countries enriched our repository with...antibody characterization. Nature Protocols. https://doi.org/10.1038/s41596-024-01095-8 Kahn, R. A., Virk, H...biomedical research. eLife, 13, e100211. https://doi.org/10.7554/eLife.100211...

...growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs/ Finding...Technologies from 2015 Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled... Kendall Morgan...

...sports, movies, and just about anything. As our organization grew, we continued this tradition. We now have...delicious) team building activity that is easy to organize. We’ll dedicate a post to these competitions in...Hack Days. During Hack Days, teams at Addgene will forgo their regular To-Do lists (other than emergency ...

...Set up your own Science by the Pint series and organize evens where scientists can grab a beer and chat...institutional knowledge and continuity - two lead organizers on each program, one junior and one senior.  ...For more info about starting your own SciComm organization and to learn about some of SITN's other initiatives...

...lab do for fun as a group? The Ellis Lab self-organizes cinema nights, game nights, and trips to eat out...flexibility and you aren’t locked into a schedule.” George Church comments on how he would go home in the ... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking the ...

... happy to have the support of the following organizations to share materials more broadly to accelerate...to industry? Over the past 8 years, several organizations have made their plasmids available beyond academia...to industry, please reach out at techtransfer@addgene.org and we will initiate a conversation with you ...

...reagent type, target species, target name, host organism, isotype, clone details, vendor, method, tissue...tissues. Nature Protocols, 17(2), Article 2. https://doi.org/10.1038/s41596-021-00644-9 Radtke, A. J., Kandov...Academy of Sciences, 117(52), 33455–33465. https://doi.org/10.1073/pnas.2018488117 More resources on the Addgene...

...collections pages, please shoot us an e-mail at help@addgene.org. We’d love to hear your feedback. Additional ...set of plasmids you’ve deposited with us. Don't forget to get yourself out there! Additional Resources...Inside Addgene Posts Additional Resources on Addgene.org Browse Our Special Collections Pages Suggest...

... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) who took the time...helps when the time comes to work with full force. George Church 1. “Initial and sustained motivation is ...Thank you again to Connie Cepko, Tom Ellis, and George Church for answering our burning questions about...

...peering right through living animals to see their organs – a liver or brain, say, or maybe a tumor. They...for optical labeling and tracking of proteins, organelles, and living cells in a spatiotemporal manner,...  Kendall Morgan...

...We're expanding our collections strategically and forging new partnerships that will bring more tools to ...Frontiers in plant science, 16, 1517321. https://doi.org/10.3389/fpls.2025.1517321 Singh, O. N., Berry, ... SARS-CoV-2. iScience, 28(10), 113498. https://doi.org/10.1016/j.isci.2025.113498...

...transducerome. Nat Chem Biol 16:841–849 . https://doi.org/10.1038/s41589-020-0535-8 Takeda S, Kadowaki S,...genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016/s0014-5793(02)02775-8 Additional resources...Find other fluorescent biosensors Resources on Addgene.org Browse the catalog of fluorescent protein plasmids...

...genome-wide, and has been widely applied in many model organisms and cell lines. Although ChIP-seq is a relatively...cells. However, when working on specific tissues or organs in whole animal models like Drosophila melanogaster...interactions with CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids...

...and space. Zebrafish have become a popular model organism because their larval stage lends itself well to...toolbox and the versatility of the zebrafish model organism, the Isacoff lab was able to link these cells ... Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use viral service Find...

..., 641–656. https://doi.org/10.1038/s41579-022-00739-4 More resources on addgene.org  Addgene's CRISPR ...distinguishing the foreign sequences and keeping the array organized. Target strand: The strand of foreign DNA that...

...Humana Press. https://doi.org/10.1385/1-59259-301-1:99 Resources on Addgene.org Addgene antibodies ...Protein Science. John Wiley & Sons, Inc. https://doi.org/10.1002/0471140864 Fox, J. D., & Waugh, D. S. (...

... responses. Cell Res 20, 34–50 (2010). https://doi.org/10.1038/cr.2009.139 Janda, A., Bowen, A., Greenspan...Function. Frontiers in Microbiology, 7. https://www.frontiersin.org/articles/10.3389/fmicb.2016.00022 Torres,...specificity. Trends in Immunology, 29(2), 91–97. https://doi.org/10.1016/j.it.2007.11.004 Xu Cao, et al. Promoting...

...extension and visit ReFigure.org. You can reach the ReFigure team at refigure@refigure.org Please support ... Learn more about Data Sharing Resources at Addgene.org Deposit Your Plasmids with Addgene Find Molecular...

... editing tool of choice for a number of model organisms: mammalian cells, yeast, drosophila, plants, worms...obligate thermophiles without the need to grow the organisms at lower than optimal temperatures. GeoCas9’s ...Blog Posts on CRISPR Additional Resources on Addgene.org Brush Up on Your CRISPR Background with Addgene...

...Searching, Reading, & Organizing Write for the Addgene Blog Resources on Addgene.org Check out Our Revamped...straight to the point. 3. Engage the reader Scientists forget that the reader is not in their head. The scientist...

... Disease that runs on behalf of the National Organization for Rare Disorders (NORD). NORD raises money...We will be collecting donations for IFOPA, an organization dedicated to raising funds and awareness of ...Potentiall HIV Treatments with CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease...

...getting a badge illustrated with my favorite microorganism (Rhodobacter sphaeroides!) at the ASM booth....with the scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution...Network at Scientific Conferences Resources on Addgene.org Check out Our Microbiology Resource Page Deposit...

...and is relatively easy to grow, it is an ideal organism for teachers looking to expand their science curriculum...professional education associations, and local organizations such as the Center for Science and Industry ...Simplifying Plant Genetic Engineering Resources on Addgene.org Find Plant Vectors at Addgene Find Simple Protocols...

...abundant biological dark matter and so the lab organized a sampling trip to Yellowstone National Park (...then, many researchers have searched for novel organisms and enzymes from these extreme environments. Gearing...GeoCas9, another thermostable Cas9 Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

...done to and for us? BMC Biology, 15, 91. https://doi.org/10.1186/s12915-017-0433-z. PMID: 29052511. Louten...Replication. Essential Human Virology, 49. https://doi.org/10.1016/B978-0-12-800947-5.00004-1 Taylor, M. W...40). Springer International Publishing. https://doi.org/10.1007/978-3-319-07758-1_2 More resources on the...

... etc.) and are ready to use on another cell or organism. This pipeline is utilized to transduce cell lines...resources on the Addgene blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral...and Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ...

...fascinating biology is found in weird and rare organisms so I found it hard to stick to vertebrates alone...afield at first and I let them pass by. But I didn’t forget: what would it be like? Then I spotted an opening...Subscribe to Career Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...

...feature database. Powering the plasmid maps on Addgene.org with SnapGene Server is useful because it helps...is to enhance Collections so that a group or organization can annotate their sequences from a shared database...sequencing at Addgene Additional Resources on Addgene.org Example plasmid analysis powered by SnapGene...