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We narrowed to 402 results for: LARGE

Showing: 101 - 120 of 402 results

  1. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...injection site is straightforward, as it targets large muscle groups (usually the thigh), though it is ...theoretically be performed anywhere on the body, somewhere large, flat, and easily accessible (like the back) is ...

  3. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...(~150 kDa) and Y-shaped because it contains two large heavy chains and two small light chains that stick...optimally binding sybody, you want to start out with a large and diverse pool of sybodies that hypothetically...because it has the advantage of starting out with a larger initial starting library size (up to 1012 members...

  4. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...self-inactivating, which is achieved by deleting a large portion of the 3'LTR in the transfer plasmid. The...through insertional mutagenesis. These risks are largely based upon the vector system used and the transgene...

  5. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...cartridges can place the spent cartridge in the large cardboard box in the copy area. These spent cartridges...

  6. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...plasmids in the library.  Spinfection If you have a large number of cells to transduce, you may want to consider...pooled libraries can be intimidating and require a large amount of experimental work, but with this guide...protocol. After functional titer is determined, larger lentiviral preps can be used for screening-scale...

  7. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...pathways and represent the largest family of human drug targets. To enable large-scale profiling of many ...by endogenous GPCR. Example applications include large-scale pharmacological profiling or interrogation...

  8. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...lentiviral-based CRISPR libraries available at Addgene. The large packaging capacity and stable expression of lentiviral... vivo CRISPR screens. AAV vectors have played a large role in these advancements, due to their many benefits...of molecular pathways. These screens have gotten larger, easier, and more comprehensive thanks to the consistent...

  9. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...novel drugs. Commercial Affairs teams make up a large and diverse group of professionals running the marketing...challenging. For instance, the client might need to get a large amount of market research done to inform a go/no-go...

  11. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...the University of Goettingen, and several other large research institutions. The following graphic depicts...institutional and lab servers specifically designed to hold large amounts of data. Although it may be useful to selectively...

  14. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...datasets are too large to transfer to a journal repository, they are likely too large to be retrieved ...their own data, with some smaller labs feeling that larger, better resourced, groups would harvest and reanalyze...

  15. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ... chemical synthesis of these molecules. To make large amounts of pure product in an affordable way, researchers...that can be assembled into individual TUs or very large (up to 20!) multi-TU constructs (Moore et al., 2016...needed in mammalian cell types, and comes with a large library of common parts to get you started quickly...build genetic circuits and would benefit from a larger library of parts but don’t need to add tags to ...multiplexed CRISPR, and chromosomal integration of larger expression cassettes (Shaw et al., 2023). Those...

  16. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...ambitious projects that require epitope tagging of a large cohort of genes (e.g. tagging genes representing...success of the epitope tagging experiment depends largely on how efficiently these components are delivered...immense possibilities to delineate the function of large protein cohorts in diverse mammalian cell types....

  18. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ... the user in different formats. This can have a large impact on downstream results. Examples: “HTML”, ... agnostic”, “model organisms”, “small genome”, “large genome”, and “organism biased scoring”. Trends: ... CRISPR design (I’m talking screens and/or very large genomes), then it is worthwhile buying a dedicated...

  19. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...offering temperature-sensitive materials, we noticed a large percentage of these shipments arrived in India at...expertise in a specific country can quickly reduce a large number of barriers. Though we haven’t solved every...

  20. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...reconstitution via mRNA trans-splicing. By splitting a large gene into smaller fragments, delivering them by ...prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various contexts...
Showing: 101 - 120 of 402 results