...29] Photoreceptors [30] Otic hair cells [31] Neurons [32, 33, 34] You can find a variety of plasmids...have been successfully transdifferentiated into neurons and β-cells [40-42]. Reprogramming and transdifferentiation...S.C. Zhang, Directed differentiation of dopamine neurons from human pluripotent stem cells. Methods Mol ...human pluripotent stem cells to cerebral cortex neurons and neural networks. Nat Protoc, 2012. 7(10): p...pluripotent stem cells to mature functional Purkinje neurons. Sci Rep, 2015. 5: p. 9232. PubMed PMID: 25782665...Direct conversion of fibroblasts to functional neurons by defined factors. Nature, 2010. 463(7284): p....

...increased iron serum levels and early-onset cataracts, is characterized by point mutations in the Iron Response....  In as little was one minute BASU generated a strong streptavidin signal (~250-fold enrichment) when...and IREB2, which post-transcriptionally regulate iron metabolism pathway transcripts. By using RaPID, ... loss of binding correlated with increased serum iron levels seen in HHCS patients. These are just three...

...somewhat similar to an academic research environment” with a strong focus on publishing, but still made it...initial resistant populations can be eradicated up-front quickly. This approach is in contrast to the current...at cancer cell resistance to ABL1 inhibitors in chronic myeloid leukemia. Bhang says the work highlighted...2015): 440-448. Pubmed PMID: 25849130. 2. Hata, Aaron N., et al. "Tumor cells can follow distinct evolutionary...

...9514755. Richard Gronostajski: NFI-related diseases of neural development Richard Gronostajski is a professor...Paula Videira, Michael McMurray, and Richard Gronostajski who each are being awarded free plasmids form...they affect brain and muscle development. The Gronostajski lab has generated mice wherein it's possible...Campus in Aurora, Colorado, USA.     Richard Gronostajski is a professor at the University of Buffalo....

...could ever do! However, there is quite a bit of upfront research you have to do to find said robot and ...into the robotics spirit include: Start using electronic multichannel and single channel pipettes. These...help keep things more organized. Cap tubes with a micronic cap sealer. This is a simple table top device ...has a roller to go over caps and push them into micronic tubes instead of pushing them in one by one. It... It seems very simplistic and it isn’t electronic but it is one of the most helpful machines I have used...

...antibody is produced from a new set of animals), and wrong application (different experiments/conditions can...that this antibody has specific binding and has a strong signal. We call the unique pattern of the antibody...7s) then the signal is weak and vice versa for a strong signal. However, exposure time and its relationship...compare exposure times of our samples. A prominent, strong signal has a shorter exposure time than the negative... neuroanatomy without the fear of referencing a wrong section. The antibody screening and development ...

... an extensive infrastructure of molecular and electronic companion technologies that has transformed the...CRISPR/Cas9-mediated genotyping, as well as our electronic reader that provides portable quantification ...Leslie Dan Faculty of Pharmacy at the University of Toronto. His research is focused on developing cell-free...biology applications for human health and the environment.     Alexander A. Green is an Assistant professor...4. Messina, Jane P., et al. "Mapping global environmental suitability for Zika virus." Elife 5 (2016):...

...conserved in mammals, expressed in neurons, and are especially abundant in neuronal synapses. shRNA silencing ...way to guide inheritance that might be less error prone than DNA replication. He too mentioned how “surprising...regulate at the DNA level and 1 at the RNA level. Udi Qimron from Tel Aviv University then spoke about using...treatment resulting in major problems in hospitals. Dr. Qimron proposes using bacterial phage vectors with CRISPR...

...images. In the early days of structural biology, Byron Rubin, a crystallographer working with Jane Richardson...solution to this problem with the development of Byron’s Bender – a mechanical device that could be used...protein’s structure into experimentally determined electron density, have been known to say something silly... in the education community that All models are wrong….Some models are useful. While a physical model ...first Cas9 model is certainly useful, it is also wrong in at least one serious way. We represented the ...

... having already been immersed in an industry environment, have a better understanding of the fast, deadline-driven...up in an industry job, being immersed in that environment sooner rather than later can be helpful. Publishing...project might be challenging. In an academic environment, surrounded by lab members who are all working...Novartis, Biogen, Amgen, New England Biolabs, Regeneron, and Genentech, among others, and the list is ...opportunity to do some great science in a supportive environment. Both paths will give you myriad opportunities...

...interview is another way to make the virtual environment more comfortable and help you to keep yourself...institution, you can get a better sense of the environment of the department, the lab setting, and how students... can also be nerve wracking to ask questions in front of other prospective students; an online meeting...by talking to current students about the work environment and social aspects of the department, you may...Options Thanks to the much more developed virtual environment resulting from the pandemic, students who are...

... In LSFM the laser light sheet, typically 2-6  microns, illuminates only one thin plane of the sample ...image acquisition is performed using cameras with electron multiplying couple-charged device (EMCCD) or complementary...of transgenic mice in which a subpopulation of neurons are brightly labeled with GFP (e.g. Thy1-YFP-H ...LSFM with multiple tissue clearing strategies and neuronal labeling methods will greatly aid our understanding...331–336. Pubmed. P. Keller, M.B. Ahrens.  (2015) Neuron, 85, 462-483. Pubmed. E.H.K. Stelzer. (2015). Nature...

...cloning capacity of ~8 kb, so either a single polycistronic vector or multiple single-gene vectors can be...endogenous pluripotency genes. Retroviral vectors are prone to incomplete silencing of reprogramming transgenes...differentiation potential. Finally, retroviruses are prone to insertional mutagenesis and random integration...retroviral vectors. This makes it possible to use a polycistronic cassette that expresses multiple reprogramming...factors can be delivered either as a single polycistronic transgene or with four different adenoviruses... ~14.5kb minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG...

...dimeric green fluorescent protein (pdDronpa) (Zhou et al., 2018). pdDronpa dimers dissociate in response to... to cyan light (500nm). By fusing the two pdDronpa domains to the sites flanking the DNA-binding cleft...binding in the dark. Upon light stimulation, the pdDronpa moieties move apart, allowing DNA binding and ...Cas9, dCas9 Gene editing Zhou et al., 2018   pdDronpa Cas9, dCas9 Gene editing, transcription Light-Dependent...photoactivatable transcription systems to induce neuronal differentiation. Nat Methods 14:963–966 . https...

...dependent on the photon budget and pushing the frontiers of fluorescence microscopy often requires more...easier and more functional in complex biological environments such as live cells and tissues (Figure 1). These...several others can be made fluorogenic using environmentally sensitive fluorophores. Overall, self-labeling...photon budget and allow us to push further the frontiers of biological imaging.  *Worried about your budget...Central PMCID: PMC4344395. 3. Lavis, Luke D., and Ronald T. Raines. "Bright ideas for chemical biology."...

...system that could do both, Addgene depositor Didier Trono and collaborators turned to the lentivirus HIV-1...experimental use. In 1996, Naldini et al. transduced rat neurons in vivo with beta-gal and other reporters, and ...vectors are incredibly popular in the lab - the Trono lab’s lentiviral packaging and envelope plasmids...Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, Trono D. Science. 1996 Apr 12;272(5259):263-7. PubMed ...Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L. J Virol. 1998 Nov;72(11):8463-71. ...

...myself in a technical support role only when I was confronted one day with a product issue in the lab. The ...the world easier. You should also demonstrate a strong willingness to help others with their problems,...sometimes comprised of several layers of support: A front-line team accepts any incoming requests and answers...because there are just so many things which can go wrong in a lengthy protocol. And customers may tell all...careers are open to experienced professionals with strong interest in the technical aspects of science and...

...virtue of liberating data from paper into an electronic environment and hence making it searchable and shareable...founder of Research Space. There are many types of electronic lab notebooks (ELNs), each with its own pros ... of Research Space, which provides the RSpace electronic lab notebook. Rory is based in Cambridge, Massachusetts...on the Addgene blog Find tips on choosing an electronic lab notebook Watch a livestream of the Minisymposium...

...CpG dinucleotides and CAC trinucleotides during neuronal maturation (Tillotson & Bird, 2019) and is a global...of symptoms in animal models, Rett syndrome is a strong translational candidate for gene replacement therapy...therapy. In 2014, RSRT brought together a strong collaboration between two gene therapy labs (Brian Kaspar... half of the mutant MECP2 RNA in each of three neuronal subtypes of the hippocampus. This technology has...Modifications in Human-Induced Pluripotent Stem Cells. Front Genet 10: . https://doi.org/10.3389/fgene.2019.00625...

...aspects of research at National Aeronautics and Space Agency, the Environmental Protection Agency, and the ...Additionally, interest groups are often pandered to when strong or overlooked when small, but data is not going...Science face deep cuts in Trump’s first budget Environmental Science and Technology: Crossing The Imaginary...having evidence’ on Trump’s wiretap claim The Chronicle of Higher Education: “March for Science” Organizer...