Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 749 results for: SOM

Showing: 101 - 120 of 749 results

  1. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... next steps in the process involve repairing chromosomal DSBs. It is important to know that cells possess...subunit DNA ligase (XRCC4-ligase IV); together with some accessory factors (PAXX, XLF), this complex holds...two-thirds chance of causing a frameshift mutation. Of some importance, the deletion can be less heterogeneous...should possess indels in the majority of their chromosomes within a day. Another factor expected to impact...or more can simultaneously introduce a pair of chromosome breaks, and could result in deletion of the intervening...Stark JM (2009) Limiting the Persistence of a Chromosome Break Diminishes Its Mutagenic Potential. PLoS...EA, Giovannangeli C, Jasin M, Brunet E (2014) Chromosomal Translocations in Human Cells Are Generated by...

  2. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...also has the flexibility to modify the E. coli chromosome, plasmid DNA or BAC DNA.  To use the lambda ...linked strains contain the Lambda Red system).  In some of these strains, expression of exo, beta and gam....   This setup can be also be used to modify chromosomal genes. After the initial editing event, the defective... defective prophage can be removed from the chromosome of the host E. coli by a second lambda red recombination... system is best suited for editing bacterial chromosomal DNA because it is easy to remove the recombineering...responsible for excision of mini-λ from the host’s chromosome. After this, mini-λ can readily be purified from... being targeted for modification: the E. coli chromosome, a plasmid, or a BAC.    Pro Tip: ssDNA substrates...

  3. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    .... These are just some of the most common parametric comparison tests along with some general statistics...long-awaited data – and it looks great! There are some exciting trends in your experimental groups, but...these designations all refer to the same test. On some occasions, there is an experimental group being ...However, it will only tell you if differences exist somewhere within your data; it does not indicate which groups...Post hoc tests Knowing that significance exists somewhere amongst multiple groups is rarely enough information...every pairwise comparison within your population? Sometimes this can’t be avoided, but if it can be, you can...working with, graphing data with a histogram is sometimes sufficient to determine normality (or lack thereof...

  4. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    .... While HaloTag reacts faster for some dyes, others, especially some nonfluorescent ligands, react more... protein, a fancy new green fluorescent protein, some affinity purification tags, another version with...with that same red fluorescent protein somebody made because they forgot it was already in the freezer… ... Common self-labeling tags Let’s take a look at some popular self-labeling tools: Tetracysteine: The ... Easy CLIP-tag 182 Benzylcytosine (BC) Some Easy Ligases Small, varies Various Few...the cells make a fluorescent protein for you. And some ligands just aren’t going to work in certain applications...check that your fusion protein is functional. In some cases, a small peptide tag like tetracysteine might...

  5. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...universities' decisions to alter postdoc salaries - in some cases reversing these decisions entirely. At Future...are indeed going ahead with their planned raises, some are taking the opportunity of the lapsed mandate...being compelled at the state level, in the case of some public institutions) to cancel plans to raise salaries... in light of the injunction. In addition, PIs at some of the institutions cancelling plans are going ahead...communication of their plans (many postdocs - and in some cases, faculty - have found out about what their...they have in their salaries and benefits, and how some institutions are paying lip service to improving... not raising salaries when all others in Boston (some of them literally across the street from these institutions...

  6. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...relationship. Pro tip: Once you are connected with someone, their email address is usually visible to you ...if you don’t know clearly why you are inviting someone to connect, perhaps you should be asking yourself...discussions.  Pro tip: Instead of connecting with someone you don’t really know, follow instead of connecting...giving you a way to connect with them in the future. Some profiles have a blue Follow button. For less famous...do an easy favor for people in your network. If someone has connected with me, I consider them fair game...updating it. LinkedIn may actually replace the resume some day (I’ve already abandoned my paper resume). It...looking for a new job! I have been able to tell when some start-ups were about to be bought by seeing certain...

  7. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...overview of some of the common challenges associated with producing and using lentivirus and offer some tips ...(i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of the reaction, and the ...transfection ratios can affect transfection efficiency. For some transgenes, transfection optimization simply is ...large inserts; a study by Kumar et al. found that in some cases, titers can drop 10-fold for every 2kb increase...vessel, such as a 3 or 5-layer flask will require some initial optimization; cell number, media volume,... volumes accordingly. While you may need to make some final adjustments, this is generally a good place...

  8. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...Consider too that some secondary antibodies can cross react with other species. For example, some goat anti-mouse...affect my experiment? In this post we will review some of the main factors that affect isotype choice. ...are using poly- or monoclonal primary antibodies. Some secondary antibodies are broad and recognize multiple...within that species, like an anti-mouse IgG antibody. Some are subclass specific, like an anti-mouse IgG2a ...also be considered when choosing an antibody. In some applications probing species-on-species, i.e. using...-mouse secondary antibodies recognize some rat primary antibodies (Erickson 1993). It is always better...

  9. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...more than one protein in your sample? Or what if something weird happened during your western and your results... targets you plan to probe for. Even using PVDF, some of your target protein will be removed from the ... first to preserve your sample. Pro tip! Because some protein is lost each time you strip a blot, you ...experiment. Remember, stripping your membrane will remove some of your sample —and harsher stripping means more... need longer incubation for effective stripping. Some blots benefit from an additional 5–10 minutes of...blotting that you’re looking for a “blank” result! If some signal remains, you may want to repeat your stripping...strip and reprobe your western blot several times — some researchers report up to ten cycles! Just remember...

  10. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...does one accomplish all of that? What follows are some tips to help you build a great poster.  Tips for...results, conclusions, and acknowledgements sections. Some other additions you might wish to include are a ...put your poster together, be aware that there are some particular styles recognized to work well. It is...are easily distinguished. The UCLA Library offers some Powerpoint based templates with different column...hang your poster too low. If it is pinned to low, some of the poster will naturally sit outside of the ...into larger circles or ovals, that are arranged in some logical pattern across the poster. This style has...accessible. Final thoughts Though we’ve just presented some standard formatting guidelines for making a scientific...

  11. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...by offering advice on areas where you might need some help. Today in our transferable skills guide: managing...being a manager: yes, being a manager means being someone’s boss. But it does not mean shouting orders and... opportunities. I still need to check myself sometimes to make sure I am delegating well, but it does...the person in front of me saying “I’ve been doing some of X’s work for a couple of weeks now”. I had no...throughout the year and in a timely manner. If someone’s performance or behavior is inadequate, you need...equally as important so don’t forget to recognize someone’s hard work and reward a job well done. Your report...to your manager. This is when having a mentor - someone more experienced to guide you - is valuable. Protect...

  12. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...experience taught me something about myself and my future career. If it wasn’t something I liked – well, I... helped prepare me to start CINSO. Here, I share some of the tips I learned along the way and hope they... favorite. This seemed like a great way to share some of the research taking place at our university with...meet people with similar interests! Especially as someone interested in doing science outreach, not only ...through developing and leading a budding nonprofit, something I had zero experience with before founding CINSO...molecular biology.      Additional Resources I asked some colleagues to share valuable life lessons they have...

  13. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...to make something the cell doesn’t need to make. Eukaryotes and some bacteria deploy proteosomes to degrade...and its related fluorescent proteins, which are sometimes used as tags for detection; however, those are...proteins. Tags for stability and solubility What are some of the hurdles to overcome in order to overexpress...there are a number of chemical and peptide-based proteosome inhibitors, glutathione S-transferase (GST), ...assess their function. More detailed information and some protocols can be found in the references provided...

  14. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...understand why some people develop the disease while others never do, but we do know that there are some genetic...disease. However, there are some emerging treatments that have shown some positive potential for treating...the first exposure to asbestos to the time when someone starts seeing – or feeling – the first mesothelioma...years, but it usually ranges from 20-50 years and sometimes longer. There is no known safe level of exposure...destruction of cancer cells. Pembrolizumab has shown some great early success in treating mesothelioma, such...

  15. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...among organisms aren’t completely understood, but some possible reasons include: Metabolic pressures ...protein may be translated at different rates. The ribosome will move quickly along regions calling for abundant... low abundance, poorly charged tRNAs. When the ribosome stalls, this may give the swiftly translated regions...mismatch between human and E. coli codon preference. Some codons commonly used in humans are not at all common...vice versa. When translating these codons, the ribosome may therefore stall at inappropriate locations...optimized for a particular organism, this will sometimes (but not always) be noted in in the “mutation”...problems surrounding codon choice, you should have some method to make sure the proteins you produce function...

  16. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...of inappropriate statistical methods, omission of some data, etc.  The firestorm that followed implementation...who wanted more time to mine their own data, with some smaller labs feeling that larger, better resourced...of the initial contributors. This is probable to some extent - but that is the price of publication. Who...viewing their data months or years before others. In some cases, typically consortia involving genomic screens...not require interaction with the data authors. In some types of research, raw data requires huge amounts...data of any sort in a form that can be parsed by someone other than the actual author. Instead, selected...should data be archived? Simplifying data access Some of the above mentioned issues could be mitigated...

  17. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ... only improved efficiency for some edits in unpredictable ways. In some cases, however, combining an evolved...find a brief discussion of some of these strategies in our CRISPR guide. Some examples include: TwinPE...perform any base-to-base conversion in principle, but some changes require multiple steps and enzymes. Prime...your prime editing tools. Here, we’ll briefly cover some major advances in prime editing tools. Scroll down...can be used with any of the PE2-5 approaches, sometimes referred to as PE2max, PE3max, etc. PE6: Specialized.... Although the addition of the RNA pseudoknot sometimes altered epegRNA expression compared to pegRNAs...

  18. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...your polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the...Melançon III. "Detection and Quantification of Ribosome Inhibition by Aminoglycoside Antibiotics in Living...Living Bacteria Using an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1...same restriction enzymes for cloning. Furthermore, some plasmids contain peptides to facilitate protein ...eukaryotic systems and instead directly engages the ribosome for translational complex assembly. PhD student...wide variety of functions via different fusions. Some applications of PUF protein fusions reported in ...Kendall Morgan’s blog post on the PUF Assembly Kit for some insights on its potential from its creators. We ...

  19. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...exclusively on. In this blog post, I will go over some advantages, disadvantages, and examples of how scientists...in which very large fragments of the bacterial chromosome (up to 100 kb) were specifically cut out via ...contain regions of homology with the bacterial chromosome fragments. CRISPR cutting was used to circumvent... the need to PCR amplify the fragments of the chromosome, which was technically challenging. Other homology...enzymes, and no scar sequences between fragments. Some of these products include: GeneArt® Seamless Cloning...particular manufacturer of the kit you will be using. Some of these products may offer advantages that Gibson...Ebenstein Y, Zhu TF. Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning...

  20. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...going through some of these top blog posts. We’d also like to use this time to hear some feedback from...effort from the Addgenies, we’re able to feature some recently deposited plasmids every quarter. The “...clones in an experiment. Read this blog post for some positive selection (ex: antibiotic selection and... Goedhart Guest blogger Joachim Goedhart shares some strategies for generating GFP fusion proteins. From...there any topics you particularly like or maybe something you’d like to see more of? Let us know by leaving...
Showing: 101 - 120 of 749 results