We narrowed to 533 results for: erf

...have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock down RNA without altering...nuclease domains characteristic of the Cas13 superfamily. Yan et al. proceeded to study orthologs from...multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated transcriptional inhibition (...

... forces scientists to use invasive measures to perform in vivo optogenetic experiments. Intracranial injections...correlated with the model’s predictions.  Three top performers, named ChRger1, ChRger2, and ChRger3, were then...minimally-invasive optogenetics While ChRgers performed well in a tissue culture dish, that doesn’t guarantee...

...still do), so we are used to the idea of general interfaces and the freedom to test out lots of combinations...define what features would allow for the best performance in the most contexts (Figure 1). Together the...the features with optimal biological performance are described as Click Biology (Howarth, 2025). ...

...step to remove the tag in order to prevent it interfering with protein function. In contrast, the polyhistidine... it is, and the greater the risk that it will interfere with protein function.  Additional Benefits of...this one developed by Dr. James Trimmer’s lab, to perform assays that they otherwise would not be able to...

...stranded? Well, that’s just for DNA – right? RNA performs almost all of its biological functions in our ...sense dsRNA and respond accordingly. While not perfect, these sensing systems work to distinguish between...blog CRISPR 101: RNA Editing with Cas13  RNA Interference in Plant Biology  ...

... from Addgene’s repository that can be used to perform screens in human cells. General information regarding...Liu at Oxford University, we are now providing a powerful CRISPR-based screening source for the Drosophila...resources for fly labs who wish to harness this powerful genome engineering tool. Bassett, et al. J Genet...locus causes that locus to be methylated. This powerful tool has been used to direct methylation to and... that high-throughput tagging projects can be performed. Schwartz, et al. Genetics. 2016. PubMed PMID:...

...Andrews et al., 2019). Finally, scientists can perform the entire process of antibody selection and maturation...recombinant antibodies. The selection process is often performed via phage display, yeast display, ribosome display...they are using and any validation experiments performed will be useful in perpetuity.  Second, plasmids...

...cloning strategy designed by synthetic biologists to perform large scale, one-tube cloning reactions. In this...courtesy of Rob Hurt. What makes MoClo systems so powerful is their modularity — many parts can be assembled...used to assemble multigene constructs capable of performing production and subsequent necessary modification...

...specific to lasers or optogenetics so cheaper, less powerful alternatives to the Master-9 that only control...published first. Because behavioral optogenetics is performed in freely moving animals, the fiber optic patch...into some of the important considerations when performing optogenetics behavioral experiments. Thank you...

... capture of chromatin interactions The Xu lab performed a proof-of-concept experiment to show that CAPTURE...original CAPTURE method. Both CAPTURE methods outperformed other 3D chromatin methods such as ChIA-PET ...Wang, K. (2018). 3C and 3C-based techniques: the powerful tools for spatial genome organization deciphering...

... here!). The only issue with these GEVIs? They perform poorly in two-photon conditions, which are necessary...the fluor via FRET. This system is particularly powerful for in vivo imaging especially since JFs can cross...type of interest. Viral pseudotyping is also a powerful tool that swaps capsid proteins from different...

...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...arms. When we select cells later, we will first perform a positive selection for the antibiotic resistance... knockout and new CRISPR tools continue to be perfected, the production of cells and mouse knockout lines...

... have a much better chance of creating a high-performing team - which is every manager’s dream. Learn ...often associate feedback with end-of-the-year performance review, but feedback should be given throughout...the year and in a timely manner. If someone’s performance or behavior is inadequate, you need to address...

...consider Gibson cloning. In this technique, you first perform a round of PCR to generate DNA fragments with 15...function while 5’ adenosine overhangs left by Taq perfectly anneal with the topoisomerase I generated overhangs...available cloning kits that one could choose from to perform these experiments. In most cases, there will be...

...thousands of designs in parallel. To do this, we performed high-throughput ‘self-targeting’ screens with ...PRIDICT2.0 score. For the best prime editing performance, we suggest that users select some of the best...learning–based model with high generalization performance. Science Advances, 5(11), eaax9249. https://doi.org...

...monomeric FPs. EGFP and other GFP family members (superfolder, Emerald, CFP, YFP, BFP, Cerulean, Turquoise,...dimerization. Importantly, all of these assays are performed in vitro. Assays to detect FP dimerization in ... 1 and 2 depend on protein concentrations. An imperfect solution is to restrict imaging analysis to cells...

...support your professional development I had a wonderfully supportive mentor and graduate office. I cannot...ages, learned a lot about the dinosaurs, and met wonderful people. When I started volunteering, I was worried...Scientists Read Emma Markham's Post on Finding Your Perfect Job After University Browse All of Our Career Posts...

...contamination could interfere with interpreting the results of transfection assays. Go forth and perform your DNA...when the kits buffers ran out. Homemade buffers performed the same as those provided with the kit. Although...

...first note that quantitative PCR (qPCR) has been a powerful tool for quantifying AAV for some time now. When...readout of your samples.  Of course, if qPCR were perfect there would be no need for ddPCR. One issue with...Furuta-Hanawa et al., 2019). Tips and tricks for performing ddPCR Clean everything You must have a clean ...

...the genome. Let’s now examine how we can locate powerful stretches of DNA known as enhancers with the help... the techniques from Table 1, provides us with powerful GPS-like techniques to explore dark matter in ...interests span machine learning, proteomics, and user interfaces for math.     Additional Resources Beyond the...