CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
Type
Blog Post
Published
Sept. 28, 2020, noon
...CRISPR systems were first discovered in bacteria, most CRISPR-based genome engineering has taken place ...finding suggests that the system may be functional in most Enterobacteriaceae.
An update to this system, called... RNA interference does not function in bacteria, most efforts to regulate gene expression were limited...adenine base editing in Streptomyces
Some of the most industrially and clinically relevant bacterial species..., yet genome engineering remains challenging for most organisms in the genus. Work from the Sun lab demonstrates...base substitutions respectively. Substitutions seem most efficient when using a nickase version of Cas9, ...