... 179 (2020). https://doi.org/10.1186/s12870-020-02388-2 Resources on Addgene.org Addgene’s Modular Cloning...modular cloning? Modular cloning, or MoClo, is an organization scheme for multi-part DNA assembly. Simply put...’s because MoClo specifically refers to the organizational schema described above, and not any particular...other non-Golden Gate cloning methods. MoClo’s organization schema MoClo can be broken down into three hierarchical...many guides/parts delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled...targeting constructs and delivering them to these organisms via another optimized platform. Gene Stacking ...

...Girardia sp., Dugesia dorotocephala, Phagocata morgani, and Phagocata gracillis. There are 11 different...developmental biologists have to extract RNA from their organism of interest, use the RNA to create a complementary... in  the labeling specific cell types or entire organs in planarians. For example, the gene Piwi labels...strain that is designated as DE3. For Phagocata morgani and Phagocata gracillis, the process is a little...E. coli. Thus, to get the dsRNA into Phagocata morgani and Phagocata gracillis, one has to purify the ..., feedback etc. please write to cuttingclass@stowers.org and if you make new plasmids for investigating...Models for learning about CRISPR Resources at Addgene.org Find plasmids from the Sánchez lab Find plasmids...

...cells or tissue slices mounted to a slide, or whole organs. Because the tissue architecture is preserved, ...from the bloodstream. Then, via a blood draw or organ harvest, the labeled cells can be analyzed using...and Future Outlook. Front Immunol 8: . https://doi.org/10.3389/fimmu.2017.01589 Ayyar BV, Arora S, Ravi...The nuts and bolts. Methods 116:51–62 . https://doi.org/10.1016/j.ymeth.2017.01.009 Crivianu-Gaita V, ...Biosensors and Bioelectronics 85:32–45 . https://doi.org/10.1016/j.bios.2016.04.091 Cruse JM, Lewis RE,...Immunology Guidebook. Elsevier, pp 17–45. https://doi.org/10.1016/B978-012198382-6/50026-1 Wang R, Xiang ... Skp. Front Cell Infect Microbiol 3: . https://doi.org/10.3389/fcimb.2013.00072 Additional resources on...

...screens. Nature Protocols, 17(9), 1903–1925. https://doi.org/10.1038/s41596-022-00700-y Hanlon, K. S., Kleinstiver... breaks. Nature Communications, 10(1). https://doi.org/10.1038/s41467-019-12449-2 Pupo, A., Fernández,... Molecular Therapy, 30(12), 3515–3541. https://doi.org/10.1016/j.ymthe.2022.09.015 Ramani, B., Rose, I...bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha, A. J., Klingler...AAV-Perturb-seq. Nature, 622(7982), 367–375. https://doi.org/10.1038/s41586-023-06570-y Wang, J., Gessler, D...Transduction and Targeted Therapy, 9(1). https://doi.org/10.1038/s41392-024-01780-w Additional resources...Using the FITS Approach Additional resources at addgene.org CRISPR pooled libraries Guide to pooled libraries...

... available in your lab or organization, and approvals from the organization overseeing your work. Good...Modeling Part 2 Five Popular Model Organisms Additional resources on Addgene.org Genome Engineering Guide CRISPR...the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular choice for administering...and to avoid potential off-target pokes to nearby organs. Restraint: scruffing Location: peritoneal cavity...Biosciences and Medicines, 08(09), 131–141. https://doi.org/10.4236/jbm.2020.89011 Shimizu, S. S. (2004). Routes...

...educating the public and organizing events. I contacted local professional organizations and nonprofits. I volunteered...Central Indiana Science Outreach, or CINSO. We organize fun science events for adults and professional...communication, editing, marketing, and website organization. My short experience with my blog helped immensely...establishments such as museums, professional organizations, and other nonprofits to gain support for CINSO...read from career guidance sources such as myidp.sciencecareers.org mentioned museums. Indianapolis is home...Browse All of Our Career Posts Resources on the Addgene.org Find Plasmids for Your Research Learn About ...

...your organization Public Speaking Time management Managing a team Conflict resolution Organization Leadership... given yourself the bathroom mirror pep talk, forgotten and then retrieved your lucky socks, persevered...than you thought! When change happens at your organization, when your boss needs someone to step up, when...likely collected some great time management and organizational skills. If you are responsible for working ...students, managing inventories in the lab, or organizing a research project, one can assume that you’ve... careers Learn about mentoring Resources on Addgene.org Check out our careers page Watch our career ...

...professional works for or with an organization to evaluate the organization’s grant readiness, identify grant...stories about a time when their passion for an organization or a program and a funding opportunity coincided...submitting it, to evaluating the capacity of an organization to receive and manage funds.  Grant professionals...targeted to the grants profession? The number of organizations, both public and private, that qualify for grants...oriented nonprofits. Like Deb, I worked for an organization in a position that required some involvement...your ideal client or job will save you and the organization(s) you serve time and resources. 3. Get a mentor...

...without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/nature24644 Glaser A, McColl B, Vadolas...Molecular Therapy - Nucleic Acids 5:e334 . https://doi.org/10.1038/mtna.2016.48 Katti A, Foronda M, Zimmerman... Nucleic Acids Research 48:2841–2852 . https://doi.org/10.1093/nar/gkaa124 Martin AS, Salamango DJ, Serebrenik...editosome complexes. Scientific Reports 9: . https://doi.org/10.1038/s41598-018-36739-9 St. Martin A, Salamango...cells. Nucleic Acids Research 46:e84–e84 . https://doi.org/10.1093/nar/gky332 Standage-Beier K, Tekel SJ, ... Nucleic Acids Research 47:e120–e120 . https://doi.org/10.1093/nar/gkz713 Additional resources on the ...reporters Browse our CRISPR blog posts Resources on Addgene.org Browse the CRISPR guide Find CRISPR plasmids...

...into the results, it’s important to note that in Morgens et al., guides were considered toxic if they were...non-specific toxicity than non-targeting guides First, Morgens et al. looked at the effect of safe-targeting guides...guides. The genome-wide CRISPR screen presented in Morgens et al. contains three types of guides: 1) targeting... reduction in on-target efficacy. Results from Morgens et al. show that genome-wide CRISPR screens are...testing thousands of guides for off-target activity, Morgens et al drew some generalizable conclusions about...Library are available from Addgene! References 1. Morgens, David W., et al. "Genome-scale measurement of ...Guide to All Things Lentivirus Resources on Addgene.org Check out this guide to pooled plasmid libraries...

...Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski,...antigen. J Gen Virol., 73 (Pt 3), 653–660. https://doi.org/10.1099/0022-1317-73-3-653. PMID 1372038. Randall.... J Gen Virol., 68 (Pt 11), 2769–2780. https://doi.org/10.1099/0022-1317-68-11-2769. PMID 2445904.   ...protein degraders. Nat Commun., 15(1), 8885. https://doi.org/10.1038/s41467-024-52871-9. PMID 39406745. Optimizing...Caenorhabditis elegans. Genetics, 228(2), iyae126. https://doi.org/10.1093/genetics/iyae126.   Gene disruption in...abscessus. J Bacteriol., 206(3), e0033523. https://doi.org/10.1128/jb.00335-23.  Bioluminescent reporting...Systems. ACS Sensors, 9(7), 3489–3495. https://doi.org/10.1021/acssensors.3c02664. ...

...Watkins, Professor at George Washington University and developer of wesharescience.org. Sharing your research...professor at George Washington University and he the developer of the WeShareScience.org platform. You...study processes for diagnosing and resolving organizational performance and often have to be able to quickly...sharing video abstracts about research: www.WeShareScience.org. WeShareScience is a free platform for ...Sharing in Research Publications Resources at Addgene.org Check out our Protocol Pages for Video Protocols...

...asks for strong organizational skills. You might think, “I’d be good at that! I organized my lab data so...important part of the application. Even within an organization, there can be variance. At Addgene, our content...explain why you are interested in working for that organization or group. This is particularly important for...want to talk about! You could describe your organizational system, your rationale for designing it the...title of the position] at [name of the company/organization]. I am a [adjective that describes you] scientist...that particular job, your commitment to the organization's mission, or your deep interest in the lab's...

...efforts and information provided by external organizations like the Human Cell Differentiation Molecules...research applications. eLife, 12, RP91645. https://doi.org/10.7554/eLife.91645 Biddle, M. S., Alende, C., ...flow cytometry. F1000Research, 13, 817. https://doi.org/10.12688/f1000research.154034.1 Laflamme, C., McKeever...disease gene C9ORF72. eLife, 8, e48363. https://doi.org/10.7554/eLife.48363 Uhlen, M., Bandrowski, A., ...antibodies. Nature Methods, 13(10), 823–827. https://doi.org/10.1038/nmeth.3995 Virk, H. S., Biddle, M. S., ...Journal of Pharmacology, 178(15), 2948–2962. https://doi.org/10.1111/bph.15467 Virk, H. S., Rekas, M. Z., Biddle...TRPA1. Scientific Reports, 9(1), 18500. https://doi.org/10.1038/s41598-019-55133-7 More resources from ...

...detection and identification using FnCas9. https://doi.org/10.1101/2020.04.07.028167 Broughton JP, Deng X,...detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Kellner MJ, Koob JG, ...nucleases. Nature Protocols 14:2986–3012 . https://doi.org/10.1038/s41596-019-0210-2 Lucia, C., Federico, ...-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch, J. N., Valois,...Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang, F., Abudayyeh,...COVID-19 using CRISPR diagnostics. https://www.broadinstitute.org/files/publications/special/COVID-19%20detection...available for COVID-19 research Resources on Addgene.org Get the basics about CRISPR in our CRISPR guide...

...samples. bioRxiv 2024.01.21.576499; doi: https://doi.org/10.1101/2024.01.21.576499.    LEA proteins protect...freezing. bioRxiv 2024.02.06.579238. doi: https://doi.org/10.1101/2024.02.06.579238.    Engineered and evolved...Biotechnology, 10.1038/s41587-023-02078-y. doi: https://doi.org/10.1038/s41587-023-02078-y.  Anzalone, A. V., Gao...Nature Biotechnology, 40(5), 731–740. doi: https://doi.org/10.1038/s41587-021-01133-w Pandey, S., Gao, X. ...Engineering, 10.1038/s41551-024-01227-1. doi:  https://doi.org/10.1038/s41551-024-01227-1   CHARM: A compact ...editor. Science, 384(6703), ado7082. doi: https://doi.org/10.1126/science.ado7082.  Nuñez, J. K., Chen, J...editing. Cell, 184(9), 2503–2519.e17. doi: https://doi.org/10.1016/j.cell.2021.03.025.    Recombinant antibodies...

...Popular Model Organisms by Alyssa Cecchetelli and Lukas Morgan There are many model organisms people are...interview including defining your research vision, organizing your vision into a chalk talk, and tips for practicing.... In this blog post, we’ve covered five model organisms: mouse, fruit fly, yeast, zebrafish, and worm.... by leaving a comment or emailing us at blog@addgene.org.  Thank you again for a fantastic year and happy...

...to me… The problem is that scientists sometimes forget how to communicate about their work with non-academic... is essential to stay in touch with the living organism I study, to be able to see it all scales. Most... a way to better understand and appreciate the organism, for both scientist and non-scientist alike. “...inner structures of delicate plants and other organisms without destroying the original, which could provide...Do you look for the beauty in the microscopic organisms on your lab bench? Let us know in the comments...Experimenting in an Open Source Lab Resources on Addgene.org Try the Plasmid Land Game ...

...analyses using ELISA. Peptides 72:4–15 . https://doi.org/10.1016/j.peptides.2015.04.012 Burnette WN (1981... Analytical Biochemistry 112:195–203 . https://doi.org/10.1016/0003-2697(81)90281-5 Pillai-Kastoori L,...for the user. J Biol Chem 295:926–939 . https://doi.org/10.1074/jbc.ra119.010472 Renart J, Reiser J, Stark...National Academy of Sciences 76:3116–3120 . https://doi.org/10.1073/pnas.76.7.3116 Towbin H, Staehelin T, Gordon...National Academy of Sciences 76:4350–4354 . https://doi.org/10.1073/pnas.76.9.4350 Yang P-C, Mahmood T (2012...trouble shooting. North Am J Med Sci 4:429 . https://doi.org/10.4103/1947-2714.100998 Additional resources on...articles related to antibodies Resources on Addgene.org Find written and video protocols Visit Addgene's...

...here yet? A quick look at your order status on addgene.org identifies the hold-up. Your Material Transfer...possible Tell them about the Golden Stopwatch! Any organization that deposits with and/or requests materials...to Addgene’s Tech Transfer Team: techtransfer@addgene.org Congratulations to the Golden Stopwatch awardees...to all of the winners so far! Don’t see your organization below? Don’t worry--we check periodically for...transfer offices. Drop us a line at techtransfer@addgene.org. Albert Einstein College of Medicine  Baylor...of commercialization Additional resources on Addgene.org Addgene Help Center: Tech Transfer Technology...