...affinity tags can make protein purification a cinch. Types of affinity tags, the proteins they work best attached...tag can be purified with several different resin types and is a fairly small tag that is unlikely to perturb...Affinity tags vs. other tags There are many different types of tags, including epitope tags (FLAG, Myc, etc....

...folding assays. Yeast vectors There are several types of yeast expression vectors. Consider your applications...mutated version of YGOI to test the different phenotypes of the specific mutations. For these purposes...effects.  And many more! We have a variety of other types of vectors for most applications used to study gene...

... GENIE Project New viral prep with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor Yulong Li...Robert Campbell New viral prep with multiple serotypes AAV-hSyn-Ribo-jGCaMP8s AAV1 Biosensor Marianne...eligible transfer plasmids and five different serotypes. Not sure what to request? Addgene's Data Hub ...

... study gene expression in many different tissue types! Although putting a gene of interest directly downstream...hedgehog gene can be overexpressed in several cell types by using different Gal4 drivers. It is much easier...of altering cell fates and generating dominant phenotypes., Development 118, 401–15 (1993). PubMed PMID...

...in weeks Figure 1: Sending out 80 FedEx envelopes in a single day -- all while maintaining social...but we're planning to soon expand to other cell types, including more physiologically-relevant systems...Getting our research in line, with a row of FedEx envelopes waiting to be shipped out of the Krogan lab at...

...profiles were then compared to that of reference cell types generated by the human fetal expression atlas. Computational...along with color coded nominated reference cell types.  (Adapted from Joung et al.)    Putting MORF...TFs which were predicted to generate unique cell types. Of the 24 TFs they tested, 22 expressed markers...

...of course, many companies selling antibodies, microscopes, and dissection tools, but there were also people...means of delivering genes to many different cell types with minimal immunological effects. AAVs are used...in experiments targeting genes to specific cell types in the brain, mapping neuronal networks, and selectively...

...direction (if relevant), and types. Types listed are standard GenBank feature types. Clicking on a particular...

...nonspecific binding. Flow specific controls The three types of flow cytometry controls we’ll discuss are single... later gates, are more likely to require FMOs. Isotypes Isotype controls are negative controls that allow...Controls like single color controls, FMOs, and isotypes can help you more easily gate your target population...

... liver, we recommend using one of the natural serotypes such as AAV9 or AAV8. Very recently an engineered...collaboration injected 1e+13 vg per mouse to get phenotypes (that’s almost 100ul/animal for the PHP.eBs that...transfer PHP.eB's enhanced CNS tropism to other serotypes, but reproducible success remains elusive.  There...

... Need Virus? Check out Addgene's Viral Service! Types of viral vectors Of the many viruses out there, ...tropism of AAV to target different tissue or cell types. AAV also requires some back up from adenovirus ...production. Fun fact: There are 57 human adenovirus types, but most vectors are based on adenovirus 5 (Ad5...

...and converted that signal into a readable value. Types of biosensors So now that we know what makes up ...AAVs make it possible. In AAV systems, different serotypes can be used to affect or determine viral tropism—the...your biosensor to specific tissues or cell (sub)types for expression and downstream monitoring. Nervous...

...solute channels typically expressed on other cell types can compensate for or bypass the dysfunction of ...signaling pathways and how they lead to neurological phenotypes observed.” Using genetically-encoded biosensors...neurons from patients with GNB1 mutations, the lab hopes to capture the signalosome of different receptors...

...cx43 GJB1 GJB2 Bioelectric states can alter cell types and patterning There is mounting evidence that signaling... certain patterning activities of entire tissue types or even organs. In frogs, ectopic expression of ...even lead to the formation of whole eyes from cell types that don’t typically give rise to eyes (Pai et al...

...variable gene expression that can impact observed phenotypes. Factors such as the growth medium, growth temperature...expression. Because pSC101 is not representative of all types of plasmids, the stabilized promoters were also ...how the stabilized promoters perform during these types of fluctuations. When cells are dividing rapidly...

... sequences available from Addgene. Dr. McMurray hopes that by demonstrating GdnHCl chemical rescue of ...These diseases are characterized by overgrowth phenotypes and neural developmental delay. Mutations in ...in loss of function or dominant negative NFIX phenotypes. Developing a rapid test for the functional ...

...different kinetic parameters. There are three GCaMP types based on their kinetics: GCaMPf (fast) GCaMPm (medium...-specific targeting. By employing various AAV serotypes, researchers can precisely direct the calcium ...sensor to specific tissues or even distinct cell subtypes for efficient expression and downstream monitoring...

...labor-intensive processes like PCR or by evaluating visual phenotypes. Improving C. elegans fluorescent protein tagging...prebuilt donor plasmid library containing several types of fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent...proteins (TagGFP2, TagBFP, TagRFP, and T2A-TurboGFP-PEST), and small epitope tags (HA, Myc, Strep tag II,...

...balancer chromosomes is that they produce dominant phenotypes visible on the flies. For example, second chromosome...stubble (sb). For a better reference of various fly phenotypes, check out the Classical Genetics Simulator website...whether stocks of mutant flies exist, whether any phenotypes have been observed from mutations in the gene...

... is transient expression sufficient? Which cell types will you be editing? Do you want to deliver the ...cell lines. Useful for difficult to transfect cell types and can be used in vivo. A common choice for conducting...Electroporation / Nucleofection Microinjection Cells Many types Single cells Cargo Nucleic acids or protein Nucleic...